Silk granular hydrogels self-reinforced with regenerated silk fibroin fibers

Soft Matter ◽  
2021 ◽  
Author(s):  
Céline Wyss ◽  
Peyman Karami ◽  
Adrien Demongeot ◽  
Pierre-Etienne Bourban ◽  
Dominique P. Pioletti
Keyword(s):  
Mechanical Properties ◽  
Silk Fibroin ◽  
High Stability ◽  
Regenerated Silk Fibroin ◽  
Strength And Toughness

Granular hydrogels with high stability, strength, and toughness are laborious to develop. Post-curing is often employed to bind microgels chemically and enhance mechanical properties. Here a unique strategy was investigated...

scholarly journals Fractionation of Regenerated Silk Fibroin and Characterization of the Fractions

Molecules ◽  
2021 ◽  
Vol 26 (20) ◽  
pp. 6317
Author(s):  
Masaaki Aoki ◽  
Yu Masuda ◽  
Kota Ishikawa ◽  
Yasushi Tamada
Keyword(s):  
Mechanical Properties ◽  
Secondary Structure ◽  
Silk Fibroin ◽  
Gel Permeation Chromatography ◽  
Precipitation Process ◽  
Regenerated Silk Fibroin ◽  
Sds Page ◽  
Fractionation Method ◽  
Nonwoven Mats

The molecular weight (MW) of regenerated silk fibroin (RSF) decreases during degumming and dissolving processes. Although MW and the MW distribution generally affect polymer material processability and properties, few reports have described studies examining the influences of MW and the distribution on silk fibroin (SF) material. To prepare different MW SF fractions, the appropriate conditions for fractionation of RSF by ammonium sulfate (AS) precipitation process were investigated. The MW and the distribution of each fraction were found using gel permeation chromatography (GPC) and SDS-polyacrylamide electrophoresis (SDS-PAGE). After films of the fractionated SFs formed, the secondary structure, surface properties, and cell proliferation of films were evaluated. Nanofiber nonwoven mats and 3D porous sponges were fabricated using the fractionated SF aqueous solution. Then, their structures and mechanical properties were analyzed. The results showed AS precipitation using a dialysis membrane at low temperature to be a suitable fractionation method for RSF. Moreover, MW affects the nanofiber and sponge morphology and mechanical properties, although no influence of MW was observed on the secondary structure or crystallinity of the fabricated materials.

Property Research of Silk Fibroin Nanofibers by Electrospinning Dissolved in CaCl2-Formid Acid

2015 ◽  
Vol 1120-1121 ◽  
pp. 331-336
Author(s):  
Yan Zhou ◽  
Hui Ying Wu
Keyword(s):  
Mechanical Properties ◽  
Silk Fibroin ◽  
Solvent System ◽  
Dissolution Time ◽  
Ethanol Treatment ◽  
Regenerated Silk Fibroin ◽  
Silk Fibroin Nanofibers ◽  
Different Content ◽  
Β Sheet ◽  
Paper Property

In this paper, property research of silk fibroin nanofibers by electrospinning dissolved in CaCl2-Formid Acid was discussed. The dissolve process, morphology, structure and mechanical properties of fibers were studied. It showed that more dissolution time was needed to dissolve silk with 1% CaCl2. The diameters of silk fibroin nanofibers decreased with the CaCl2 contents increased and the surface of 6%5% fibers was more uniform and smooth. After ethanol treatment, strong peak at 1628cm-1, 1517cm-1 and 1231cm-1 was corresponding to β-sheet. It was important to discover that silk fibroin was weakly destroyed in CaCl2-FA solvent system, and the regenerated silk fibroin nanofibers had better mechanical properties. The stress of 6%5% fibers was nearly 3 times to traditional way. With different content of CaCl2, the stress of 6%5% fibers was nearly 2 times to 6%1% fibers.

scholarly journals Tunable Structures and Properties of Electrospun Regenerated Silk Fibroin Mats Annealed in Water Vapor at Different Times and Temperatures

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Xiangyu Huang ◽  
Suna Fan ◽  
Alhadi Ibrahim Mohammed Altayp ◽  
Yaopeng Zhang ◽  
Huili Shao ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Water Vapor ◽  
Silk Fibroin ◽  
Conformational Transition ◽  
Breaking Strength ◽  
Electrospinning Technique ◽  
Structures And Properties ◽  
Regenerated Silk Fibroin ◽  
Vapor Annealing ◽  
Breaking Energy

Regenerated silk fibroin (SF) mats were fabricated using electrospinning technique, followed by mild water vapor annealing to effectively tune the structures and improve the mechanical properties of the mats at different annealing times and temperatures. The breaking strength and the breaking energy of the mats treated with water vapor at 65°C for 12 h reached 6.0 MPa and 171.7 J/kg, respectively. The conformational transition of the SF mats was significantly influenced by the treating temperature, while the influence of time was comparatively limited. The influence is consistent with the time-temperature equivalent principle and would be helpful for the preparation of water-vapor-annealed silk-based biomaterials for various applications.

scholarly journals Anti-Inflammatory Properties of Injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels

Biomolecules ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1456
Author(s):  
Isabel Matos Oliveira ◽  
Cristiana Gonçalves ◽  
Myeong Eun Shin ◽  
Sumi Lee ◽  
Rui Luis Reis ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Mechanical Properties ◽  
Silk Fibroin ◽  
Therapeutic Efficacy ◽  
Composite Structures ◽  
Gelation Time ◽  
Polymeric Materials ◽  
Gellan Gum ◽  
Traditional Use

Rheumatoid arthritis is a rheumatic disease for which a healing treatment does not presently exist. Silk fibroin has been extensively studied for use in drug delivery systems due to its uniqueness, versatility and strong clinical track record in medicine. However, in general, natural polymeric materials are not mechanically stable enough, and have high rates of biodegradation. Thus, synthetic materials such as gellan gum can be used to produce composite structures with biological signals to promote tissue-specific interactions while providing the desired mechanical properties. In this work, we aimed to produce hydrogels of tyramine-modified gellan gum with silk fibroin (Ty–GG/SF) via horseradish peroxidase (HRP), with encapsulated betamethasone, to improve the biocompatibility and mechanical properties, and further increase therapeutic efficacy to treat rheumatoid arthritis (RA). The Ty–GG/SF hydrogels presented a β-sheet secondary structure, with gelation time around 2–5 min, good resistance to enzymatic degradation, a suitable injectability profile, viscoelastic capacity with a significant solid component and a betamethasone-controlled release profile over time. In vitro studies showed that Ty–GG/SF hydrogels did not produce a deleterious effect on cellular metabolic activity, morphology or proliferation. Furthermore, Ty–GG/SF hydrogels with encapsulated betamethasone revealed greater therapeutic efficacy than the drug applied alone. Therefore, this strategy can provide an improvement in therapeutic efficacy when compared to the traditional use of drugs for the treatment of rheumatoid arthritis.

scholarly journals Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials?

2021 ◽  
Vol 22 (7) ◽  
pp. 3391
Author(s):  
Sylwia Grabska-Zielińska ◽  
Alina Sionkowska ◽  
Ewa Olewnik-Kruszkowska ◽  
Katarzyna Reczyńska ◽  
Elżbieta Pamuła
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Physicochemical Properties ◽  
Silk Fibroin ◽  
Three Dimensional ◽  
Microscopy Imaging ◽  
Maximum Deformation ◽  
One Step ◽  
Chitosan Blends ◽  
Fourier Transfer Infrared Spectroscopy

The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC). DAS was commercially available, while DAC was obtained by one-step synthesis. Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis. Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells. The results showed that the properties of the scaffolds based on silk fibroin, collagen and chitosan can be modified by chemical cross-linking with DAS and DAC. It was found that DAS and DAC have different influence on the properties of biopolymeric scaffolds. Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC. Additionally, scaffolds cross-linked with DAS exhibited higher biocompatibility than those cross-linked with DAC. However, the obtained results showed that both types of scaffolds can provide the support required in regenerative medicine and tissue engineering. The scaffolds presented in the present work can be potentially used in bone tissue engineering to facilitate healing of small bone defects.

scholarly journals Investigation of the biological activity, mechanical properties and wound healing application of a novel scaffold based on lignin–agarose hydrogel and silk fibroin embedded zinc chromite nanoparticles

RSC Advances ◽  
2021 ◽  
Vol 11 (29) ◽  
pp. 17914-17923
Author(s):  
Reza Eivazzadeh-Keihan ◽  
Hooman Aghamirza Moghim Aliabadi ◽  
Fateme Radinekiyan ◽  
Mohammad Sobhani ◽  
Farzane khalili ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Wound Healing ◽  
Biological Activity ◽  
Silk Fibroin ◽  
Agarose Hydrogel ◽  
Novel Scaffold ◽  
Fibroin Solution ◽  
Zinc Chromite

Given the important aspects of wound healing approaches, in this work, an innovative biocompatible nanobiocomposite scaffold was designed and prepared based on cross-linked lignin–agarose hydrogel, extracted silk fibroin solution, and zinc chromite (ZnCr2O4) nanoparticles.

Silk-based hybrid microfibrous mats as guided bone regeneration membranes

2021 ◽  
Author(s):  
Mi Wu ◽  
Zhengyi Han ◽  
Wen Liu ◽  
Jinrong Yao ◽  
Bingjiao Zhao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cell Adhesion ◽  
Bone Regeneration ◽  
Silk Fibroin ◽  
Guided Bone Regeneration ◽  
Regenerated Silk Fibroin ◽  
Marrow Mesenchymal Stem Cells ◽  
Cell Adhesion And Proliferation

LAPONITE® (LAP) nanoplatelets were incorporated within a regenerated silk fibroin (RSF) microfibrous mat via electrospinning, which exhibited better cell adhesion and proliferation of bone marrow mesenchymal stem cells (BMSCs) than the pristine RSF ones.

Graphene oxide/alginate/silk fibroin composite as a novel bionanostructure with improved blood compatibility, less toxicity and enhanced mechanical properties

2020 ◽  
Vol 248 ◽  
pp. 116802 ◽  
Author(s):  
Reza Eivazzadeh-Keihan ◽  
Fateme Radinekiyan ◽  
Hamid Madanchi ◽  
Hooman Aghamirza Moghim Aliabadi ◽  
Ali Maleki
Keyword(s):  
Mechanical Properties ◽  
Graphene Oxide ◽  
Silk Fibroin ◽  
Blood Compatibility
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