scholarly journals Naphthalimide-based peptide conjugate for concurrent imaging and apoptosis induction in cancer cells by utilizing endogenous hydrogen sulfide

2021 ◽  
Author(s):  
Khashti B Joshi ◽  
Sandeep Verma ◽  
Deepshikha Tewari ◽  
Naibedya Chattopadhyay ◽  
Narendra Singh ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Tumor Growth ◽  
Cancer Cells ◽  
The Other ◽  
Apoptosis Induction ◽  
Other Hand ◽  
Tumor Growth And Metastasis ◽  
Concurrent Imaging

The excessive production of endogenous hydrogen sulfide (H2S) in cancer cells leads to enhanced tumor growth and metastasis. On the other hand, decreased endogenous H2S suppresses tumor growth. The reported...

The cholesterol metabolite 27-hydroxycholesterol increases the secretion of extracellular vesicles which promote breast cancer progression

Endocrinology ◽  
2021 ◽  
Author(s):  
Amy E Baek ◽  
Natalia Krawczynska ◽  
Anasuya Das Gupta ◽  
Svyatoslav Victorovich Dvoretskiy ◽  
Sixian You ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Tumor Growth ◽  
Cancer Cells ◽  
Extracellular Vesicles ◽  
Cancer Progression ◽  
Myeloid Cells ◽  
Breast Cancer Progression ◽  
Label Free ◽  
Tumor Growth And Metastasis ◽  
Promote Breast Cancer

Abstract Cholesterol has been implicated in the clinical progression of breast cancer, a disease that continues to be the most commonly diagnosed cancer in women. Previous work has identified the cholesterol metabolite, 27-hydroxycholesterol (27HC), as a major mediator of the effects of cholesterol on breast tumor growth and progression. 27HC can act as an estrogen receptor (ER) modulator to promote the growth of ERα+ tumors, and a liver x receptor (LXR) ligand in myeloid immune cells to establish an immune-suppressive program. In fact, the metastatic properties of 27HC require the presence of myeloid cells, with neutrophils (PMNs) being essential for the increase in lung metastasis in murine models. In an effort to further elucidate the mechanisms by which 27HC alters breast cancer progression, we made the striking finding that 27HC promoted the secretion of extracellular vesicles (EVs), a diverse assortment of membrane bound particles that include exosomes. The resulting EVs had a size distribution that was skewed slightly larger, compared to EVs generated by treating cells with vehicle. The increase in EV secretion and size was consistent across three different subtypes: primary murine PMNs, RAW264.7 monocytic cells and 4T1 murine mammary cancer cells. Label-free analysis of 27HC-EVs indicated that they had a different metabolite composition to those from vehicle-treated cells. Importantly, 27HC-EVs from primary PMNs promoted tumor growth and metastasis in two different syngeneic models, demonstrating the potential role of 27HC induced EVs in the progression of breast cancer. EVs from PMNs were taken up by cancer cells, macrophages and PMNs, but not T cells. Since EVs did not alter proliferation of cancer cells, it is likely that their pro-tumor effects are mediated through interactions with myeloid cells. Interestingly, RNA-seq analysis of tumors from 27HC-EV treated mice do not display significantly altered transcriptomes, suggesting that the effects of 27HC-EVs occur early on in tumor establishment and growth. Future work will be required to elucidate the mechanisms by which 27HC increases EV secretion, and how these EVs promote breast cancer progression. Collectively however, our data indicate that EV secretion and content can be regulated by a cholesterol metabolite, which may have detrimental effects in terms of disease progression, important findings given the prevalence of both breast cancer and hypercholesterolemia.

Cancer cells uptake porphyrinsviaheme carrier protein 1

2013 ◽  
Vol 17 (01n02) ◽  
pp. 36-43 ◽  
Author(s):  
Kazuhiro Hiyama ◽  
Hirofumi Matsui ◽  
Masato Tamura ◽  
Osamu Shimokawa ◽  
Mariko Hiyama ◽  
...  
Keyword(s):  
Laser Beam ◽  
Cancer Cells ◽  
The Other ◽  
Carrier Protein ◽  
Hematoporphyrin Derivative ◽  
Lower Intensity ◽  
First Report ◽  
Over Expression ◽  
Other Hand ◽  
Eximer Laser

Although exogenous porphyrin accumulation in cancer cells is important for the success of photodynamic therapies, the mechanism is not clear. We hypothesized that a newly reported transporter, heme carrier protein 1 (HCP1), is highly expressed in cancer cells, and transports porphyrins into the cells. We investigated the following three unknowns: whether cancer cells take up hematoporphyrin derivative via HCP1, whether HCP1 is involved in photodynamic therapies, and whether cancer cells highly express HCP1. First, when HCP1-overexpressed cells were treated with hematoporphyrin derivative and then exposed to an eximer laser beam, they emitted a significantly higher intensity of hematoporphyrin derivative fluorescence and became more susceptible to the laser beam than control. Second, when three other types of cancer cells with silenced HCP1 were treated with hematoporphyrin derivative and then exposed to the laser beam, they emitted a significantly lower intensity of hematoporphyrin derivative fluorescence. Third, non-cancer cells slightly expressed HCP1; on the other hand, the three other types of cancer cells clearly expressed HCP1. These results indicated that cancer cells uptake hematoporphyrin derivative via HCP1 and over-expression of HCP1 increases the efficacy of photodynamic therapies by increasing porphyrin accumulation in the cells. This is the first report about a transporter of porphyrin in cancer cells.

scholarly journals Expression of core 3 synthase in human pancreatic cancer cells suppresses tumor growth and metastasis

2013 ◽  
pp. n/a-n/a ◽  
Author(s):  
Prakash Radhakrishnan ◽  
Paul M. Grandgenett ◽  
Ashley M. Mohr ◽  
Stephanie K. Bunt ◽  
Fang Yu ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Tumor Growth ◽  
Cancer Cells ◽  
Human Pancreatic Cancer ◽  
Pancreatic Cancer Cells ◽  
Tumor Growth And Metastasis

Tumor growth and metastasis can be inhibited by maintaining genomic stability in cancer cells

2015 ◽  
Vol 9 (1) ◽  
pp. 57-62 ◽  
Author(s):  
Yi Liang ◽  
Qisheng Feng ◽  
Jian Hong ◽  
Futuo Feng ◽  
Yi Sang ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Cancer Cells ◽  
Genomic Stability ◽  
Tumor Growth And Metastasis
Sign in / Sign up

Export Citation Format

Share Document