scholarly journals Folding and duplex formation in mixed sequence recognition-encoded m-phenylene ethynylene polymers

2021 ◽  
Author(s):  
Giulia Iadevaia ◽  
Jonathan A. Swain ◽  
Diego Núñez-Villanueva ◽  
Andrew D. Bond ◽  
Christopher A. Hunter
Keyword(s):  
Phenylene Ethynylene ◽  
One Pot ◽  
Sequence Recognition ◽  
Mixed Sequence ◽  
Duplex Formation

One pot oligomerisation reactions give access to families of oligomers that allow facile analysis of folding propensity and assessment of suitability for sequence-selective duplex formation.

scholarly journals Mixed-Sequence Recognition of Double-Stranded DNA Using Enzymatically Stable Phosphorothioate Invader Probes

Molecules ◽  
2015 ◽  
Vol 20 (8) ◽  
pp. 13780-13793 ◽  
Author(s):  
Brooke Anderson ◽  
Saswata Karmakar ◽  
Patrick Hrdlicka
Keyword(s):  
Sequence Recognition ◽  
Double Stranded Dna ◽  
Mixed Sequence

Scalable One-Pot - Liquid-Phase Oligonucleotide Synthesis for Model Network Hydrogels

2020 ◽  
Author(s):  
Guido Creusen ◽  
Cecilia Oluwadunsin Akintayo ◽  
Katja Schumann ◽  
Andreas Walther
Keyword(s):  
Liquid Phase ◽  
Solid Phase ◽  
Adenine Nucleotides ◽  
Building Blocks ◽  
Oligonucleotide Synthesis ◽  
One Pot ◽  
Model Network ◽  
Dna And Rna ◽  
Sequence Recognition ◽  
Sequential Coupling

Solid-phase oligonucleotide synthesis (SPOS) based on phosphoramidite chemistry is currently the most widespread technique for DNA and RNA synthesis, but suffers from scalability limitations and high reagent consumption. Liquid-phase oligonucleotide synthesis (LPOS) uses soluble polymer supports and has the potential of being scalable. However, at present, LPOS requires 3 separate reaction steps and 4-5 precipitation steps per nucleotide addition. Moreover, long acid exposure times during the deprotection step degrade sequences with high A-content (adenine) due to depurination and chain cleavage. In this work, we present the first one-pot liquid-phase DNA synthesis technique, which allows the addition of one nucleotide in a one-pot reaction of sequential coupling, oxidation and deprotection, followed by a single precipitation step. Furthermore, we demonstrate how to suppress depurination during the addition of adenine nucleotides. We showcase the potential of this technique to prepare high-purity 4-arm PEG‑T<sub>20</sub> (T = thymine) and 4-arm PEG-A<sub>20</sub>building blocks in multi-gram scale. Such complementary 4-arm PEG-DNA building blocks reversibly self-assemble into supramolecular model network hydrogels, and facilitate the elucidation of bond lifetimes. These model network hydrogels exhibit new levels of mechanical properties, high stability at room temperature (melting at 44 ‎°C), and thus open up pathways to next-generation, scalable DNA-materials programmable through sequence recognition and available for macroscale applications<i>.</i>

scholarly journals Identification and Characterization of Second-Generation Invader Locked Nucleic Acids (LNAs) for Mixed-Sequence Recognition of Double-Stranded DNA

2013 ◽  
Vol 78 (19) ◽  
pp. 9560-9570 ◽  
Author(s):  
Sujay P. Sau ◽  
Andreas S. Madsen ◽  
Peter Podbevsek ◽  
Nicolai K. Andersen ◽  
T. Santhosh Kumar ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Second Generation ◽  
Sequence Recognition ◽  
Double Stranded Dna ◽  
Mixed Sequence ◽  
Locked Nucleic Acids ◽  
Identification And Characterization

Double elimination protocol for the synthesis of arylene ethynylenes containing heteroaromatic rings

2005 ◽  
Vol 83 (6-7) ◽  
pp. 716-727 ◽  
Author(s):  
Akihiro Orita ◽  
Fangguo Ye ◽  
Govindarajulu Babu ◽  
Tomohiro Ikemoto ◽  
Junzo Otera
Keyword(s):  
Aldol Reaction ◽  
Phenylene Ethynylene ◽  
Elimination Reaction ◽  
One Pot

The double elimination reaction of β-substituted sulfones offers a versatile strategy for synthesis of arylene ethynylene kits containing heteroaromatic rings. A sequence of aldol reaction between α-sulfonyl carbanion and aldehyde, trapping the resulting aldolate to give β-substituted sulfone, and double elimination of this intermediate can be integrated in one pot. This protocol allows thiophene, pyridine, and ferrocene units to be accommodated in phenylene ethynylene arrays.Key words: arylene–ethynylenes, heteroaromatic rings, ferrocene, double elimination, sulfones.

One pot synthesis of fluorescent π-conjugated materials: immobilization of phenylene–ethynylene polyelectrolytes in silica confined ionogels

2011 ◽  
Vol 21 (35) ◽  
pp. 13588 ◽  
Author(s):  
Nathalie Cheminet ◽  
Thibaut Jarrosson ◽  
Jean-Pierre Lère-Porte ◽  
Françoise Serein-Spirau ◽  
Luiz Cury ◽  
...  
Keyword(s):  
Phenylene Ethynylene ◽  
Conjugated Materials ◽  
One Pot ◽  
One Pot Synthesis
Sign in / Sign up

Export Citation Format

Share Document