scholarly journals Diverse Protein Manipulations with Genetically Encoded Glutamic Acid Benzyl Ester

2021 ◽  
Author(s):  
Xiaochen Yang ◽  
Hui Miao ◽  
Ruotong Xiao ◽  
Luyao Wang ◽  
Yan Zhao ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Genetic Code ◽  
Benzyl Ester ◽  
Biological Research ◽  
Site Specific ◽  
Specific Modification ◽  
Genetic Code Expansion ◽  
Therapeutics Development

Site-specific modification of proteins has significantly advanced the use of protein in biological research and therapeutics development. Among various strategies aimed at this end, genetic code expansion (GCE) allows structurally...

scholarly journals Cover Picture: Debugging Eukaryotic Genetic Code Expansion for Site-Specific Click-PAINT Super-Resolution Microscopy (Angew. Chem. Int. Ed. 52/2016)

2016 ◽  
Vol 55 (52) ◽  
pp. 15931-15931
Author(s):  
Ivana Nikić ◽  
Gemma Estrada Girona ◽  
Jun Hee Kang ◽  
Giulia Paci ◽  
Sofya Mikhaleva ◽  
...  
Keyword(s):  
Genetic Code ◽  
Super Resolution ◽  
Site Specific ◽  
Genetic Code Expansion ◽  
Super Resolution Microscopy

scholarly journals Debugging Eukaryotic Genetic Code Expansion for Site‐Specific Click‐PAINT Super‐Resolution Microscopy

2016 ◽  
Vol 55 (52) ◽  
pp. 16172-16176 ◽  
Author(s):  
Ivana Nikić ◽  
Gemma Estrada Girona ◽  
Jun Hee Kang ◽  
Giulia Paci ◽  
Sofya Mikhaleva ◽  
...  
Keyword(s):  
Genetic Code ◽  
Super Resolution ◽  
Site Specific ◽  
Genetic Code Expansion ◽  
Super Resolution Microscopy

Expanding the genetic code for site-specific labelling of tobacco mosaic virus coat protein and building biotin-functionalized virus-like particles

2014 ◽  
Vol 50 (30) ◽  
pp. 4007-4009 ◽  
Author(s):  
F. C. Wu ◽  
H. Zhang ◽  
Q. Zhou ◽  
M. Wu ◽  
Z. Ballard ◽  
...  
Keyword(s):  
Coat Protein ◽  
Tobacco Mosaic Virus ◽  
Genetic Code ◽  
Mosaic Virus ◽  
Site Specific ◽  
Virus Like Particles ◽  
Specific Modification ◽  
Virus Coat Protein ◽  
Virus Coat ◽  
Specific Labelling

Building biotin-functionalized virus-like particles by combining a genetic code expanding technology and site specific modification of tobacco mosaic virus coat protein.

scholarly journals Bifunctional Non-Canonical Amino Acids: Combining Photo-Crosslinking with Click Chemistry

Biomolecules ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 578 ◽  
Author(s):  
Jan-Erik Hoffmann
Keyword(s):  
Amino Acids ◽  
Genetic Code ◽  
Click Chemistry ◽  
Protein Interactions ◽  
Site Specific ◽  
Genetic Code Expansion

Genetic code expansion is a powerful tool for the study of protein interactions, as it allows for the site-specific incorporation of a photoreactive group via non-canonical amino acids. Recently, several groups have published bifunctional amino acids that carry a handle for click chemistry in addition to the photo-crosslinker. This allows for the specific labeling of crosslinked proteins and therefore the pulldown of peptides for further analysis. This review describes the properties and advantages of different bifunctional amino acids, and gives an overview about current and future applications.

Site-specific ubiquitylation and SUMOylation using genetic-code expansion and sortase

2019 ◽  
Vol 15 (3) ◽  
pp. 276-284 ◽  
Author(s):  
Maximilian Fottner ◽  
Andreas-David Brunner ◽  
Verena Bittl ◽  
Daniel Horn-Ghetko ◽  
Alexander Jussupow ◽  
...  
Keyword(s):  
Genetic Code ◽  
Site Specific ◽  
Genetic Code Expansion

scholarly journals Pyrrolysyl-tRNA-Synthetase: Methanogenese und Gencode-Erweiterung

BIOspektrum ◽  
2021 ◽  
Vol 27 (6) ◽  
pp. 616-619
Author(s):  
Nikolaj Georg Koch ◽  
Nediljko Budisa
Keyword(s):  
Amino Acids ◽  
Genetic Code ◽  
Methanogenic Archaea ◽  
Trna Synthetase ◽  
Side Chains ◽  
Site Specific ◽  
Genetic Code Expansion ◽  
The Way

AbstractPyrrolysyl-tRNA synthetase (PylRS) is an enzyme of some methanogenic Archaea for the natural incorporation of pyrrolysine into proteins. The discovery of PylRS as a natural tool for genetic code expansion paved the way for site-specific incorporation of non-canonical amino acids (ncAAs) into proteins, with versatile side chains useful in biotechnology. Almost 20 years after the discovery, we describe the journey which led to three distinct classes of PylRSs with unique ncAA recognitions.

Synthesis of Site-Specific Radiolabeled Antibodies for Radioimmunotherapy via Genetic Code Expansion

2016 ◽  
Vol 27 (10) ◽  
pp. 2460-2468 ◽  
Author(s):  
Yiming Wu ◽  
Hua Zhu ◽  
Bo Zhang ◽  
Fei Liu ◽  
Jingxian Chen ◽  
...  
Keyword(s):  
Genetic Code ◽  
Site Specific ◽  
Genetic Code Expansion ◽  
Radiolabeled Antibodies
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