scholarly journals A facile combinatorial approach to construct a ratiometric fluorescent sensor: application for the real-time sensing of cellular pH changes

2021 ◽  
Author(s):  
Eiji Nakata ◽  
Hisaaki Hirose ◽  
Khongorzul Gerelbaatar ◽  
Jan Vincent Valenzuela Arafiles ◽  
Zhengxiao Zhang ◽  
...  
Keyword(s):  
Real Time ◽  
Intracellular Ph ◽  
Living Cell ◽  
Fluorescent Sensor ◽  
Combinatorial Approach ◽  
Comprehensive Understanding ◽  
Cellular Space ◽  
Ph Changes ◽  
Cellular Environment ◽  
Dynamics Process

Realtime monitoring of the cellular environment, such as the intracellular pH, in a defined cellular space provides comprehensive understanding of the dynamics process in living cell. Considering the limitation of...

A New Fluorescent Salen-uranyl Sensor for the Sub-ppm Detection of Chemical Warfare Agents

2020 ◽  
Vol 24 (20) ◽  
pp. 2378-2382
Author(s):  
Andrea Pappalardo ◽  
Chiara M.A. Gangemi ◽  
Rosa Maria Toscano ◽  
Giuseppe Trusso Sfrazzetto
Keyword(s):  
Real Time ◽  
Fluorescent Sensor ◽  
Chemical Warfare Agents ◽  
Optical Response ◽  
Chemical Warfare ◽  
Lethal Effects ◽  
Strong Affinity ◽  
Chemical Attack ◽  
Fluorescence Measurements ◽  
Warfare Agents

Real-time sensing of Chemical Warfare Agents (CWAs) is today a crucial topic to prevent the lethal effects of a terroristic chemical attack. For this reason, the development of efficient, selective, sensitive and reversible sensoristic devices, able to detect by optical response ppm levels of these compounds, is strongly required. Here, the synthesis of a new fluorescent sensor based on a salen-uranyl scaffold, functionalized with two bodipy moieties, and its application for the detection of sub-ppm levels of CWAs is reported. Detection properties were evaluated by fluorescence measurements and selectivity tests demonstrated the strong affinity for CWAs.

Real-time tracking lysosomal pH changes under heatstroke and redox stress with a novel near-infrared emissive probe

Talanta ◽  
2021 ◽  
Vol 228 ◽  
pp. 122184
Author(s):  
Qingfeng Xia ◽  
Shumin Feng ◽  
Jiaxin Hong ◽  
Guoqiang Feng
Keyword(s):  
Real Time ◽  
Near Infrared ◽  
Redox Stress ◽  
Lysosomal Ph ◽  
Ph Changes ◽  
Real Time Tracking ◽  
Emissive Probe

A novel rhodamine-based colorimetric and fluorescent sensor for Hg2+ in water matrix and living cell

2016 ◽  
Vol 232 ◽  
pp. 28-36 ◽  
Author(s):  
Miaomiao Hong ◽  
Xiangyu Lu ◽  
Yuhua Chen ◽  
Dongmei Xu
Keyword(s):  
Living Cell ◽  
Fluorescent Sensor ◽  
Water Matrix

scholarly journals Energy Metabolism and Intracellular pH Alteration in Neural Spheroids Carrying Down syndrome

Biomedicines ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 1741
Author(s):  
Alena Kashirina ◽  
Alena Gavrina ◽  
Emil Kryukov ◽  
Vadim Elagin ◽  
Yuliya Kolesova ◽  
...  
Keyword(s):  
Down Syndrome ◽  
Fluorescence Microscopy ◽  
Minimally Invasive ◽  
Real Time ◽  
Intracellular Ph ◽  
Normal Karyotype ◽  
3D Models ◽  
Brain Diseases ◽  
Fluorescence Lifetimes ◽  
Metabolic Analysis

Brain diseases including Down syndrome (DS/TS21) are known to be characterized by changes in cellular metabolism. To adequately assess such metabolic changes during pathological processes and to test drugs, methods are needed that allow monitoring of these changes in real time with minimally invasive effects. Thus, the aim of our work was to study the metabolic status and intracellular pH of spheroids carrying DS using fluorescence microscopy and FLIM. For metabolic analysis we measured the fluorescence intensities, fluorescence lifetimes and the contributions of the free and bound forms of NAD(P)H. For intracellular pH assay we measured the fluorescence intensities of SypHer-2 and BCECF. Data were processed with SPCImage and Fiji-ImageJ. We demonstrated the predominance of glycolysis in TS21 spheroids compared with normal karyotype (NK) spheroids. Assessment of the intracellular pH indicated a more alkaline intracellular pH in the TS21 spheroids compared to NK spheroids. Using fluorescence imaging, we performed a comprehensive comparative analysis of the metabolism and intracellular pH of TS21 spheroids and showed that fluorescence microscopy and FLIM make it possible to study living cells in 3D models in real time with minimally invasive effects.

scholarly journals New near-infrared rhodamine dyes with large Stokes shifts for sensitive sensing of intracellular pH changes and fluctuations

2018 ◽  
Vol 54 (55) ◽  
pp. 7625-7628 ◽  
Author(s):  
Yibin Zhang ◽  
Shuai Xia ◽  
Mingxi Fang ◽  
Wafa Mazi ◽  
Yanbo Zeng ◽  
...  
Keyword(s):  
Intracellular Ph ◽  
Near Infrared ◽  
Sensitive Detection ◽  
Amine Group ◽  
Ph Changes ◽  
Stokes Shifts ◽  
Rhodamine Dyes

New near-infrared rhodamine dyes with large Stokes shifts were developed and applied for sensitive detection of cellular pH changes and fluctuations by incorporating an additional amine group with fused rings.

A fluorescent sensor based on aggregation-induced emission: highly sensitive detection of hydrazine and its application in living cell imaging

The Analyst ◽  
2018 ◽  
Vol 143 (18) ◽  
pp. 4298-4305 ◽  
Author(s):  
Jiabin Qiu ◽  
Yunxiang Chen ◽  
Shengjie Jiang ◽  
Hongyu Guo ◽  
Fafu Yang
Keyword(s):  
Living Cell ◽  
Cell Imaging ◽  
Fluorescent Sensor ◽  
Sensitive Detection ◽  
Aggregation Induced Emission ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
Living Cell Imaging

A fluorescent sensor based on aggregation-induced emission for amines was prepared and its highly sensitive detection of hydrazine and application in living cell imaging were investigated in detail.

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