scholarly journals Rapid and multiplex preparation of engineered Mycobacterium smegmatis porin A (MspA) nanopores for single molecule sensing and sequencing

2021 ◽  
Author(s):  
Shuanghong Yan ◽  
Liying Wang ◽  
Xiaoyu Du ◽  
Shanyu Zhang ◽  
Sha Wang ◽  
...  
Keyword(s):  
Single Molecule ◽  
Mycobacterium Smegmatis ◽  
Recent Developments

Acknowledging its unique conical lumen structure, Mycobacterium smegmatis porin A (MspA) was the first type of nanopore that has successfully sequenced DNA. Recent developments of nanopore single molecule chemistry have...

Molecular Motors: Force and Movement Generated by Single Myosin II Molecules

Physiology ◽  
2002 ◽  
Vol 17 (5) ◽  
pp. 213-218 ◽  
Author(s):  
Caspar Rüegg ◽  
Claudia Veigel ◽  
Justin E. Molloy ◽  
Stephan Schmitz ◽  
John C. Sparrow ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Single Molecule ◽  
Molecular Motors ◽  
Molecular Motor ◽  
Myosin Ii ◽  
Force Production ◽  
Myosin Isoforms ◽  
Single Molecule Level ◽  
Recent Developments ◽  
Muscle Myosin

Muscle myosin II is an ATP-driven, actin-based molecular motor. Recent developments in optical tweezers technology have made it possible to study movement and force production on the single-molecule level and to find out how different myosin isoforms may have adapted to their specific physiological roles.

scholarly journals Preparation of Mycobacterium smegmatis porin A (MspA) nanopores for single molecule sensing of nucleic acids

2021 ◽  
Vol 7 (5) ◽  
pp. 355-364
Author(s):  
Wang Yuqin ◽  
◽  
Fan Pingping ◽  
Zhang Shanyu ◽  
Yan Shuanghong ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Single Molecule ◽  
Mycobacterium Smegmatis

scholarly journals Methods for protein delivery into cells: from current approaches to future perspectives

2020 ◽  
Vol 48 (2) ◽  
pp. 357-365
Author(s):  
Chalmers Chau ◽  
Paolo Actis ◽  
Eric Hewitt
Keyword(s):  
Single Molecule ◽  
Cell Biology ◽  
Protein Delivery ◽  
Mammalian Cells ◽  
Cultured Cells ◽  
Cellular Damage ◽  
Future Perspectives ◽  
Chemically Modified ◽  
Recent Developments ◽  
Direct Delivery

The manipulation of cultured mammalian cells by the delivery of exogenous macromolecules is one of the cornerstones of experimental cell biology. Although the transfection of cells with DNA expressions constructs that encode proteins is routine and simple to perform, the direct delivery of proteins into cells has many advantages. For example, proteins can be chemically modified, assembled into defined complexes and subject to biophysical analyses prior to their delivery into cells. Here, we review new approaches to the injection and electroporation of proteins into cultured cells. In particular, we focus on how recent developments in nanoscale injection probes and localized electroporation devices enable proteins to be delivered whilst minimizing cellular damage. Moreover, we discuss how nanopore sensing may ultimately enable the quantification of protein delivery at single-molecule resolution.

scholarly journals Stochastically pumped adaptation and directional motion of molecular machines

2018 ◽  
Vol 115 (38) ◽  
pp. 9405-9413 ◽  
Author(s):  
R. Dean Astumian
Keyword(s):  
Single Molecule ◽  
Molecular Motors ◽  
High Efficiency ◽  
Probability Distributions ◽  
Molecular Machines ◽  
High Energy ◽  
Stochastic Thermodynamics ◽  
Recent Developments ◽  
Directional Motion ◽  
External Fluctuations

Recent developments in synthetic molecular motors and pumps have sprung from a remarkable confluence of experiment and theory. Synthetic accomplishments have facilitated the ability to design and create molecules, many of them featuring mechanically bonded components, to carry out specific functions in their environment—walking along a polymeric track, unidirectional circling of one ring about another, synthesizing stereoisomers according to an external protocol, or pumping rings onto a long rod-like molecule to form and maintain high-energy, complex, nonequilibrium structures from simpler antecedents. Progress in the theory of nanoscale stochastic thermodynamics, specifically the generalization and extension of the principle of microscopic reversibility to the single-molecule regime, has enhanced the understanding of the design requirements for achieving strong unidirectional motion and high efficiency of these synthetic molecular machines for harnessing energy from external fluctuations to carry out mechanical and/or chemical functions in their environment. A key insight is that the interaction between the fluctuations and the transition state energies plays a central role in determining the steady-state concentrations. Kinetic asymmetry, a requirement for stochastic adaptation, occurs when there is an imbalance in the effect of the fluctuations on the forward and reverse rate constants. Because of strong viscosity, the motions of the machine can be viewed as mechanical equilibrium processes where mechanical resonances are simply impossible but where the probability distributions for the state occupancies and trajectories are very different from those that would be expected at thermodynamic equilibrium.

scholarly journals Microfluidic systems for rapid antibiotic susceptibility tests (ASTs) at the single-cell level

2020 ◽  
Vol 11 (25) ◽  
pp. 6352-6361 ◽  
Author(s):  
Kaixiang Zhang ◽  
Shangshang Qin ◽  
Sixuan Wu ◽  
Yan Liang ◽  
Jinghong Li
Keyword(s):  
Single Cell ◽  
Antibiotic Treatment ◽  
Single Molecule ◽  
Antibiotic Susceptibility ◽  
Single Cell Level ◽  
Microfluidic Systems ◽  
Cell Level ◽  
Single Molecule Level ◽  
Recent Developments ◽  
Susceptibility Tests

Recent developments of microfluidics-based antibiotic susceptibility tests (ASTs) at the single-cell or single-molecule level are summarized for guiding antibiotic treatment.

scholarly journals Recent Developments in the Interactions of Classic Intercalated Ruthenium Compounds: [Ru(bpy)2dppz]2+ and [Ru(phen)2dppz]2+ with a DNA Molecule

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 769 ◽  
Author(s):  
Fuchao Jia ◽  
Shuo Wang ◽  
Yan Man ◽  
Parveen Kumar ◽  
Bo Liu
Keyword(s):  
Single Molecule ◽  
Specific Binding ◽  
Equilibrium Constants ◽  
Binding Modes ◽  
Dna Molecule ◽  
Intercalated Compounds ◽  
Ruthenium Compounds ◽  
Recent Developments ◽  
Dynamic Assembly ◽  
Dna Helix

[Ru(bpy)2dppz]2+ and [Ru(phen)2dppz]2+ as the light switches of the deoxyribose nucleic acid (DNA) molecule have attracted much attention and have become a powerful tool for exploring the structure of the DNA helix. Their interactions have been intensively studied because of the excellent photophysical and photochemical properties of ruthenium compounds. In this perspective, this review describes the recent developments in the interactions of these two classic intercalated compounds with a DNA helix. The mechanism of the molecular light switch effect and the selectivity of these two compounds to different forms of a DNA helix has been discussed. In addition, the specific binding modes between them have been discussed in detail, for a better understanding the mechanism of the light switch and the luminescence difference. Finally, recent studies of single molecule force spectroscopy have also been included so as to precisely interpret the kinetics, equilibrium constants, and the energy landscape during the process of the dynamic assembly of ligands into a single DNA helix.

Recent Developments in Artificial Molecular-Machine–Based Active Nanomaterials and Nanosystems

MRS Bulletin ◽  
2008 ◽  
Vol 33 (3) ◽  
pp. 226-231 ◽  
Author(s):  
Tony Jun Huang
Keyword(s):  
Single Molecule ◽  
Interdisciplinary Research ◽  
Recent Progress ◽  
High Strain ◽  
Molecular Machines ◽  
High Impact ◽  
Molecular Machine ◽  
Mechanical Motion ◽  
Structures And Properties ◽  
Recent Developments

AbstractArtificial molecular machines capable of converting chemical, electrochemical, and photochemical energy into mechanical motion represent a high-impact, fast-growing field of interdisciplinary research. These molecular-scale systems utilize a “bottom-up” technology centered upon the design and manipulation of molecular assemblies and are potentially capable of delivering efficient actuation at length scales dramatically smaller than traditional microscale actuators. As actuation materials, molecular machines have many advantages, such as high strain (40%–60%), high force and energy densities, and the capability to maintain their actuation properties from the level of a single molecule to the macroscale. These advantages have inspired researchers to develop molecular-machine–based active nanomaterials and nanosystems, including electroactive and photoactive polymers. This article will discuss the structures and properties of artificial molecular machines, as well as review recent progress on efforts to move molecular machines from solution to surfaces to devices.

DETECTING AND MANIPULATING SINGLE MOLECULES WITH STM

NANO ◽  
2006 ◽  
Vol 01 (01) ◽  
pp. 15-33 ◽  
Author(s):  
J. G. HOU ◽  
AIDI ZHAO
Keyword(s):  
Single Molecule ◽  
Scanning Tunneling Microscope ◽  
Light Emission ◽  
Single Molecules ◽  
Final Part ◽  
Scanning Tunneling ◽  
Tunneling Microscope ◽  
Recent Advances ◽  
Recent Developments ◽  
Single Molecule Manipulation

Scanning tunneling microscope (STM) is a powerful and unique tool for study single molecules. We review recent advances in single-molecule characterizations including direct STM imaging and I–V spectroscopy, dI/dV spectroscopy and mapping, and d2I/dV2 spectroscopy and mapping. Some recent experiments of STM-excited single-molecule light emission are also introduced. In the final part, recent developments of single-molecule manipulation with the STM as well as the applications are discussed.

Lanthanide single molecule magnets: progress and perspective

2015 ◽  
Vol 44 (9) ◽  
pp. 3923-3929 ◽  
Author(s):  
Peng Zhang ◽  
Li Zhang ◽  
Jinkui Tang
Keyword(s):  
Single Molecule ◽  
Single Molecule Magnets ◽  
Recent Developments

The recent developments in lanthanide SMMs highlighted in this frontier article indicate the directions to which further synthetic efforts should be focused.

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