scholarly journals Liposome leakage and increased cellular permeability induced by guanidine-based oligomers: effects of liposome composition on liposome leakage and human lung epithelial barrier permeability

RSC Advances ◽  
2021 ◽  
Vol 11 (51) ◽  
pp. 32000-32011
Author(s):  
Yeonjeong Ha ◽  
Yerim Koo ◽  
Seon-Kyung Park ◽  
Ga-Eun Kim ◽  
Han Bin Oh ◽  
...  
Keyword(s):  
Human Lung ◽  
Epithelial Barrier ◽  
Barrier Permeability ◽  
Lung Epithelial ◽  
Cellular Permeability

In this study, liposome leakage using different liposome compositions and increased cellular permeability of human lung monolayer models induced by PHMG and PHMB were investigated.

scholarly journals Effect of sub-chronic exposure to cigarette smoke, electronic cigarette and waterpipe on human lung epithelial barrier function

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Baishakhi Ghosh ◽  
Hermes Reyes-Caballero ◽  
Sevcan Gül Akgün-Ölmez ◽  
Kristine Nishida ◽  
Lakshmana Chandrala ◽  
...  
Keyword(s):  
Cigarette Smoke ◽  
Barrier Function ◽  
Chronic Exposure ◽  
Human Lung ◽  
Electronic Cigarette ◽  
Epithelial Barrier ◽  
Epithelial Barrier Function ◽  
Lung Epithelial

Legionella pneumophila induced microRNA expression in human lung epithelial cells

Pneumologie ◽  
2010 ◽  
Vol 64 (S 03) ◽  
Author(s):  
B Schmeck ◽  
B Dolniak ◽  
I Pollock ◽  
C Schulz ◽  
W Bertrams ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Legionella Pneumophila ◽  
Human Lung ◽  
Microrna Expression ◽  
Lung Epithelial Cells ◽  
Lung Epithelial ◽  
Human Lung Epithelial Cells

scholarly journals Effect of Alpha-1 Antitrypsin on CFTR Levels in Primary Human Airway Epithelial Cells Grown at the Air-Liquid-Interface

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2639
Author(s):  
Frauke Stanke ◽  
Sabina Janciauskiene ◽  
Stephanie Tamm ◽  
Sabine Wrenger ◽  
Ellen Luise Raddatz ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Human Lung ◽  
Liquid Interface ◽  
Lung Epithelial Cells ◽  
Western Blots ◽  
Mesenchymal Transition ◽  
Protein Levels ◽  
Lung Epithelial ◽  
Cftr Protein ◽  
Air Liquid Interface

The cystic fibrosis transmembrane conductance regulator (CFTR) gene is influenced by the fundamental cellular processes like epithelial differentiation/polarization, regeneration and epithelial–mesenchymal transition. Defects in CFTR protein levels and/or function lead to decreased airway surface liquid layer facilitating microbial colonization and inflammation. The SERPINA1 gene, encoding alpha1-antitrypsin (AAT) protein, is one of the genes implicated in CF, however it remains unknown whether AAT has any influence on CFTR levels. In this study we assessed CFTR protein levels in primary human lung epithelial cells grown at the air-liquid-interface (ALI) alone or pre-incubated with AAT by Western blots and immunohistochemistry. Histological analysis of ALI inserts revealed CFTR- and AAT-positive cells but no AAT-CFTR co-localization. When 0.5 mg/mL of AAT was added to apical or basolateral compartments of pro-inflammatory activated ALI cultures, CFTR levels increased relative to activated ALIs. This finding suggests that AAT is CFTR-modulating protein, albeit its effects may depend on the concentration and the route of administration. Human lung epithelial ALI cultures provide a useful tool for studies in detail how AAT or other pharmaceuticals affect the levels and activity of CFTR.

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