scholarly journals Long term storage of miRNA at room and elevated temperatures in a silica sol–gel matrix

RSC Advances ◽  
2021 ◽  
Vol 11 (50) ◽  
pp. 31505-31510
Author(s):  
Rajat Chauhan ◽  
Theodore S. Kalbfleisch ◽  
Chinmay S. Potnis ◽  
Meenakshi Bansal ◽  
Mark W. Linder ◽  
...  
Keyword(s):  
Room Temperature ◽  
Elevated Temperatures ◽  
Sol Gel ◽  
Silica Sol ◽  
Rapid Degradation ◽  
Global Impact ◽  
Long Term Storage ◽  
Term Storage ◽  
Over Time

Storage of biospecimens in their near native environment at room temperature can have a transformative global impact, however, this remains an arduous challenge to date due to the rapid degradation of biospecimens over time.

NANOEMULSIONS BASED ON LIPOIC ACID DERIVATIVES WITH VARIOUS ALCOHOLS: PRODUCTION AND INFLUENCE ON FUNCTIONAL ACTIVITY OF PLATELETS

2020 ◽  
pp. 461-464
Author(s):  
A. Sinebryukhova ◽  
A. Shipelova ◽  
E. Darnotuk ◽  
A. Chekanov ◽  
O. Baranova ◽  
...  
Keyword(s):  
Particle Size ◽  
Arachidonic Acid ◽  
Lipoic Acid ◽  
Encapsulation Efficiency ◽  
Room Temperature ◽  
Optimal Conditions ◽  
Pluronic F68 ◽  
Long Term Storage ◽  
Term Storage

The optimal conditions were selected for obtaining homogeneous nanoemulsions (NE) of lipoic acid conjugates (LA-conjugates) based on Pluronic F68 (1,8%) with a particle size not exceeding 400 nm, characterized by 97±2% encapsulation efficiency of substances in nanoparticles (NP). A heterogeneous NE (polydispersity index, PDI>0,3) with the derivative of LA and myo-inositol based on phosphatidylcholine (PC, C = 3 mg/ml) was also obtained consisting of 2 particle fractions: 20–70 nm (27%) and 122–212 nm (73%). The obtained NEs with LA-conjugates based on Pluronic F68 and PC were stable during long-term storage (more than 12 months) at room temperature. The effect of the obtained NEs of LA-conjugates on platelet aggregation (Pt) caused by arachidonic acid (AA) was determined, and a mechanism of their action was proposed.

scholarly journals Intraindividual variability over time in plasma biomarkers of inflammation and effects of long-term storage

2014 ◽  
Vol 25 (8) ◽  
pp. 969-976 ◽  
Author(s):  
Sheetal Hardikar ◽  
Xiaoling Song ◽  
Mario Kratz ◽  
Garnet L. Anderson ◽  
Patricia L. Blount ◽  
...  
Keyword(s):  
Intraindividual Variability ◽  
Plasma Biomarkers ◽  
Long Term Storage ◽  
Term Storage ◽  
Over Time

scholarly journals HMGB1 concentration measurements in trauma patients: assessment of pre-analytical conditions and sample material

2019 ◽  
Vol 26 (1) ◽  
Author(s):  
William Ottestad ◽  
Ingrid N. Rognes ◽  
Erlend Skaga ◽  
Cassandra Frisvoll ◽  
Guttorm Haraldsen ◽  
...  
Keyword(s):  
Western Blot ◽  
Room Temperature ◽  
Trauma Patients ◽  
Plasma Samples ◽  
Sample Material ◽  
Limits Of Agreement ◽  
Freeze Thaw ◽  
Long Term Storage ◽  
Term Storage

Abstract Background HMGB1 is a mediator of systemic inflammation in sepsis and trauma, and a promising biomarker in many diseases. There is currently no standard operating procedure for pre-analytical handling of HMGB1 samples, despite that pre-analytical conditions account for a substantial part of the overall error rate in laboratory testing. We hypothesized that the considerable variations in reported HMGB1 concentrations and kinetics in trauma patients could be partly explained by differences in pre-analytical conditions and choice of sample material. Methods Trauma patients (n = 21) admitted to a Norwegian Level I trauma center were prospectively included. Blood was drawn in K2EDTA coated tubes and serum tubes. The effects of delayed centrifugation were evaluated in samples stored at room temperature for 15 min, 3, 6, 12, and 24 h respectively. Plasma samples subjected to long-term storage in − 80 °C and to repeated freeze/thaw cycles were compared with previously analyzed samples. HMGB1 concentrations in simultaneously acquired arterial and venous samples were also compared. HMGB1 was assessed by standard ELISA technique, additionally we investigated the suitability of western blot in both serum and plasma samples. Results Arterial HMGB1 concentrations were consistently lower than venous concentrations in simultaneously obtained samples (arterial = 0.60 x venous; 95% CI 0.30–0.90). Concentrations in plasma and serum showed a strong linear correlation, however wide limits of agreement. Storage of blood samples at room temperature prior to centrifugation resulted in an exponential increase in plasma concentrations after ≈6 h. HMGB1 concentrations were fairly stable in centrifuged plasma samples subjected to long-term storage and freeze/thaw cycles. We were not able to detect HMGB1 in either serum or plasma from our trauma patients using western blotting. Conclusions Arterial and venous HMGB1 concentrations cannot be directly compared, and concentration values in plasma and serum must be compared with caution due to wide limits of agreement. Although HMGB1 levels in clinical samples from trauma patients are fairly stable, strict adherence to a pre-analytical protocol is advisable in order to protect sample integrity. Surprisingly, we were unable to detect HMGB1 utilizing standard western blot analysis.

Influence of Air Abrasion and Long-term Storage on the Bond Strength of Self-etching Adhesives to Dentin

10.2341/06-61 ◽  
2007 ◽  
Vol 32 (3) ◽  
pp. 217-224 ◽  
Author(s):  
F. M. G. França ◽  
A. J. S. Santos ◽  
J. R. Lovadino
Keyword(s):  
Bond Strength ◽  
Aluminum Oxide ◽  
Clinical Relevance ◽  
Air Abrasion ◽  
Long Term Storage ◽  
Term Storage ◽  
Over Time

Clinical Relevance The adhesiveness of self-etching systems, used with aluminum oxide air abrasion to dentin, decreases over time.

scholarly journals Properties of Fiberboard Overpack Material in the 9975 Shipping Package Following Thermal Aging

2007 ◽  
Author(s):  
W. L. Daugherty
Keyword(s):  
Service Life ◽  
Physical Properties ◽  
Thermal Aging ◽  
Impact Resistance ◽  
Storage Conditions ◽  
Radioactive Material ◽  
Long Term Storage ◽  
Term Storage ◽  
Over Time

Many radioactive material shipping packages incorporate cane fiberboard overpacks for thermal insulation and impact resistance. Mechanical, thermal and physical properties have been measured on cane fiberboard following thermal aging in several temperature/humidity environments. Several of the measured properties change significantly over time in the more severe environments, while other properties are relatively constant. These properties continue to be tracked, with the goal of developing a model for predicting a service life under long-term storage conditions.

Long-Term Microhardness Evolution in Ti-Ni Shape Memory Alloys Processed by Severe Cold Rolling

2008 ◽  
Vol 584-586 ◽  
pp. 1039-1044
Author(s):  
Andrey Korotitskiy ◽  
K.E. Inaekyan ◽  
Vladimir Brailovski ◽  
Sergey Prokoshkin
Keyword(s):  
Cold Rolling ◽  
Shape Memory ◽  
Room Temperature ◽  
Structural Changes ◽  
Vickers Microhardness ◽  
Time Dependent ◽  
Logarithmic Strain ◽  
Long Term Storage ◽  
Term Storage

Ti-50.26at.%Ni shape memory alloy samples were subjected to cold rolling (CR) with true strains encompassing from moderate (logarithmic strain e=0.25) to severe (e=2.1) deformation. СR with e = 0.5 and more initiated a partial austenite amorphization. The evaluation of structural changes in the material during its long-term storage was performed using Vickers microhardness (HV) technique. It was shown that during storage at room temperature up to 9 months, microhardness varied following a dome-shaped trend, thus reflecting commonly encountered interaction between two concurrent time-dependent phenomena, the first responsible for the material hardening, and the second, for the material softening. To represent such phenomena, a simple mathematical model was proposed and experimentally validated.

scholarly journals Development of cryopreservation methods of seed of Nepeta cataria

2021 ◽  
Vol 102 (2) ◽  
pp. 37-42
Author(s):  
Margarita Ishmuratova ◽  
◽  
Damirzhan Baigarayev ◽  
Saltanat Tleukenova ◽  
Elena Gavrilkova ◽  
...  
Keyword(s):  
Liquid Nitrogen ◽  
Room Temperature ◽  
Seed Viability ◽  
Positive Temperature ◽  
Seed Rate ◽  
Nepeta Cataria ◽  
Long Term Storage ◽  
Medical Plant ◽  
Term Storage

This article presents the summarized data on cryopreservation of seeds of the medical plant Nepeta cataria. Cryopreservation is a highly promising method for saving of seed materials, allowing to organize long-term storage without viability loss. The purpose of present work is to optimize conditions of cryopreservation of seed materials of Nepeta cataria. Assessment of seed survival rate in the storage showed a linear decrease in seed viability and energy of germination. After 30 months of storage at the low positive temperature (+5 ºC) in paper pack seed rate decreased to 12.0 % and energy of germination to 11.2 %; after 4 years of storage seeds lost viability. During conduction of research the type of container, condition of thawing, optimal moisture of seeds and cryoprotectants are optimized. The optimal container for cryopreservation in liquid nitrogen was plastic cryo tubes; defrosting at room temperature. The best seed rate is found at moisture 3 %; the best cryoprotectant was glucose, the optimal concentration was 15 %. The result of the research is used for creation of the long-term storage medicinal cultures’ seed bank in the liquid nitrogen.

scholarly journals Impaired performance of SARS-CoV-2 antigen-detecting rapid tests at elevated temperatures

2021 ◽  
Author(s):  
Verena Haage ◽  
Edmilson Ferreira de Oliveira-Filho ◽  
Andres Moreira-Soto ◽  
Arne Kühne ◽  
Carlo Fischer ◽  
...  
Keyword(s):  
Cell Culture ◽  
Elevated Temperatures ◽  
False Negative ◽  
Storage Conditions ◽  
World Health ◽  
Cell Culture Supernatant ◽  
Analytical Sensitivity ◽  
Long Term Storage ◽  
Term Storage

AbstractRapid antigen-detecting tests (Ag-RDTs) can complement molecular diagnostics for COVID-19. The recommended temperature for storage of SARS-CoV-2 Ag-RDTs ranges between 5-30°C. In many countries that would benefit from SARS-CoV-2 Ag-RDTs, mean temperatures exceed 30°C. We assessed analytical sensitivity and specificity of eleven commercially available SARS-CoV-2 Ag-RDTs using different storage and operational temperatures, including (i) long-term storage and testing at recommended conditions, (ii) recommended storage conditions followed by 10 minutes exposure to 37°C and testing at 37°C and (iii) 3 weeks storage followed by testing at 37°C. The limits of detection of SARS-CoV-2 Ag-RDTs under recommended conditions ranged from 8.2×105-7.9×107 genome copies/ml of infectious SARS-CoV-2 cell culture supernatant. Despite long-term storage at recommended conditions, 10 minutes pre-incubation of Ag-RDTs and testing at 37°C resulted in about ten-fold reduced sensitivity for 46% of SARS-CoV-2 Ag-RDTs, including both Ag-RDTs currently listed for emergency use by the World Health Organization. After 3 weeks of storage at 37°C, 73% of SARS-CoV-2 Ag-RDTs exhibited about ten-fold reduced sensitivity. Specificity of SARS-CoV-2 Ag-RDTs using cell culture-derived human coronaviruses HCoV-229E and HCoV-OC43 was not affected by storage and testing at 37°C. In summary, short- and long-term exposure to elevated temperatures likely impairs sensitivity of several SARS-CoV-2 Ag-RDTs that may translate to false-negative test results at clinically relevant virus concentrations compatible with inter-individual transmission. Ensuring appropriate transport and storage conditions, and development of tests that are more robust across temperature fluctuations will be important for accurate use of SARS-CoV-2 Ag-RDTs in tropical settings.

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