scholarly journals Preparative separation of gallic acid from Fallopia aubertii using middle-pressure chromatogram isolated gel coupled with reversed-phase chromatography with hydrophilic groups

RSC Advances ◽  
2021 ◽  
Vol 11 (44) ◽  
pp. 27276-27282
Author(s):  
Dijun Ji ◽  
Qi Wang ◽  
Huan Wang ◽  
Qian Ma ◽  
Min Wang ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Gallic Acid ◽  
Reversed Phase ◽  
Tibetan Medicine ◽  
Purification Process ◽  
Preparative Separation ◽  
Reversed Phase Chromatography ◽  
Traditional Tibetan Medicine

Fallopia aubertii (L. Henry) Holub is a traditional Tibetan medicine to treat gout, but the research on its chemical composition is limited, probably due to the complex purification process.

Comparative study of methods for the isolation and purification of bovine κ-casein and its hydrolysis by chymosin

1996 ◽  
Vol 63 (1) ◽  
pp. 61-71 ◽  
Author(s):  
Kate P. Coolbear ◽  
David F. Elgar ◽  
Tim Coolbear ◽  
John S. Ayers
Keyword(s):  
Chemical Composition ◽  
Gel Electrophoresis ◽  
Reversed Phase ◽  
Reversed Phase Chromatography ◽  
Isolation And Purification ◽  
N Terminus ◽  
Hydrolysis Of ◽  
Single Batch ◽  
Made In ◽  
Final Purification

Summaryκ-Casein was purified from a single batch of whole acid casein (κ-A variant) using different methods in order to compare their merits in producing a purified material with a carbohydrate and phosphate heterogeneity representative of the whole κ-casein complement in milk. Ion-exchange methods of purification gave products of higher purity than precipitation techniques involving final purification by ethanol fractionation, but all methods resulted in κ-caseins of apparently similar heterogeneity and chemical composition. The purified κ-caseins were hydrolysed with chymosin and the derived macropeptides isolated. These were all virtually identical as determined by reversed-phase chromatography and gel electrophoresis. Some observations on chymosin hydrolysis of κ-casein were made. In addition to formation of the major para-κ-casein (Glu1–Phe105) and macropeptide (Met106–Val169), chymosin hydrolysis at pH 6·6 also resulted in two minor para-κ-caseins with N-termini corresponding to Phe18 and Ser33 of κ-casein. At pH 5·5 and 4·5 para-κ-casein was rapidly hydrolysed into at least six fragments, one of which had an N-terminus corresponding to Trp76 of κ-casein. At pH 6·6, 5·5 and 4·5 the κ-casein macropeptide was stable to chymosin, but at pH 2·3 it was hydrolysed by chymosin into fragments with N-termini corresponding to Met106, He125, Ala138, Val139, Thr145 and Glu147 of κ-casein.

Polyvinyl Alcohol/Chitosan Composite Hydrogels with Sustained Release of Traditional Tibetan Medicine for Promoting Chronic Diabetic Wound Healing

2021 ◽  
Author(s):  
Xingyu Chen ◽  
Zuxin Wang ◽  
Shan Gao ◽  
Wanlin Zhang ◽  
Hanwen Gong ◽  
...  
Keyword(s):  
Wound Healing ◽  
Polyvinyl Alcohol ◽  
Sustained Release ◽  
Skin Diseases ◽  
Tibetan Medicine ◽  
Diabetic Wound ◽  
Composite Hydrogels ◽  
Diabetic Wound Healing ◽  
Traditional Tibetan Medicine ◽  
Chitosan Composite

The Tibetan eighteen flavor dangshen pills (TEP) are composed of 18 traditional Tibetan medicines, which are commonly used in the treatment of skin diseases in the Tibetan medicine system. They...

Authenticating and distinguishing the eight species of traditional Tibetan medicine “Meiduoluomi” by microscopic technique

2009 ◽  
Vol 72 (10) ◽  
pp. 727-736 ◽  
Author(s):  
Jing An ◽  
Jie Li ◽  
Jiang-Gang Wang ◽  
Zhi-Feng Zhang ◽  
Chu Chen ◽  
...  
Keyword(s):  
Tibetan Medicine ◽  
Microscopic Technique ◽  
Traditional Tibetan Medicine

Mobile phase effects in reversed-phase chromatography

1979 ◽  
Vol 185 ◽  
pp. 129-152 ◽  
Author(s):  
Wayne R. Melander ◽  
Avi Nahum ◽  
Csaba Horváth
Keyword(s):  
Mobile Phase ◽  
Reversed Phase ◽  
Reversed Phase Chromatography ◽  
Mobile Phase Effects
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