scholarly journals Biomaterial design strategies to address obstacles in craniomaxillofacial bone repair

RSC Advances ◽  
2021 ◽  
Vol 11 (29) ◽  
pp. 17809-17827
Author(s):  
Marley J. Dewey ◽  
Brendan A. C. Harley
Keyword(s):  
Bone Repair ◽  
Bone Defects ◽  
Design Strategies

There exist many challenges in the process of regenerating craniomaxillofacial bone defects, thus biomaterials must be designed to overcome these.

Regeneration of Bone Defects Using Bioactive Glass Combined with Adipose-derived Mesenchymal Stem Cells. An experimental in vivo study

2019 ◽  
Vol 70 (6) ◽  
pp. 1983-1987
Author(s):  
Cristian Trambitas ◽  
Anca Maria Pop ◽  
Alina Dia Trambitas Miron ◽  
Dorin Constantin Dorobantu ◽  
Flaviu Tabaran ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bioactive Glass ◽  
Bone Repair ◽  
Bone Defects ◽  
Calvarial Bone ◽  
Specific Protocol ◽  
Repair Mechanisms ◽  
Male Wistar Rats

Large bone defects are a medical concern as these are often unable to heal spontaneously, based on the host bone repair mechanisms. In their treatment, bone tissue engineering techniques represent a promising approach by providing a guide for osseous regeneration. As bioactive glasses proved to have osteoconductive and osteoinductive properties, the aim of our study was to evaluate by histologic examination, the differences in the healing of critical-sized calvarial bone defects filled with bioactive glass combined with adipose-derived mesenchymal stem cells, compared to negative controls. We used 16 male Wistar rats subjected to a specific protocol based on which 2 calvarial bone defects were created in each animal, one was filled with Bon Alive S53P4 bioactive glass and adipose-derived stem cells and the other one was considered control. At intervals of one week during the following month, the animals were euthanized and the specimens from bone defects were histologically examined and compared. The results showed that this biomaterial was biocompatible and the first signs of osseous healing appeared in the third week. Bone Alive S53P4 bioactive glass could be an excellent bone substitute, reducing the need of bone grafts.

Enhancement of Bone Healing by Local Administration of Carbon Nanotubes Functionalized with Sodium Hyaluronate in Rat Tibiae

2017 ◽  
Vol 204 (3-4) ◽  
pp. 137-149 ◽  
Author(s):  
Vanessa B. Andrade ◽  
Marcos A. Sá ◽  
Renato M. Mendes ◽  
Paulo A. Martins-Júnior ◽  
Gerluza A.B. Silva ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Bone Repair ◽  
Bone Matrix ◽  
Sodium Hyaluronate ◽  
Repair Process ◽  
Bone Defects ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein ◽  
Bone Trabeculae ◽  
Endothelial Growth Factor

It has been reported that carbon nanotubes (CNTs) serve as nucleation sites for the deposition of bone matrix and cell proliferation. Here, we evaluated the effects of multi-walled CNTs (MWCNTs) on bone repair of rat tibiae. Furthermore, because sodium hyaluronate (HY) accelerates bone restoration, we associated CNTs with HY (HY-MWCNTs) in an attempt to boost bone repair. The bone defect was created by a 1.6-mm-diameter drill. After 7 and 14 days, tibiae were processed for histological and morphometric analyses. Immunohistochemistry was used to evaluate the expression of vascular endothelial growth factor (VEGF) in bone defects. Expression of osteocalcin (OCN), bone morphogenetic protein-2 (BMP-2), and collagen I (Col I) was assessed by real-time PCR. Histomorphometric analysis showed a similar increase in the percentage of bone trabeculae in tibia bone defects treated with HY and HY-MWCNTs, and both groups presented more organized and thicker bone trabeculae than nontreated defects. Tibiae treated with MWCNTs or HY- MWCNTs showed a higher expression of VEGF. Treatment with MWCNTs or HY-MWCNTs increased the expression of molecules involved in the bone repair process, such as OCN and BMP-2. Also, HY- and MWCNT-treated tibiae had an increased expression of Col I. Thus, it is tempting to conclude that CNTs associated or not with other materials such as HY emerged as a promising biomaterial for bone tissue engineering.

scholarly journals Preliminary in Vivo Evaluation of Bone Healing in Critical Size Bone Defects Implanted with an Injectable Calcium Phosphate Bone Cement (Osteopaste)

2017 ◽  
Vol 16 (1) ◽  
Author(s):  
Che Nor Zarida Che Seman ◽  
Zamzuri Zakaria ◽  
Zunariah Buyong ◽  
Mohd Shukrimi Awang ◽  
Ahmad Razali Md Ralib @ Md Raghib
Keyword(s):  
Calcium Phosphate ◽  
Bone Cement ◽  
Bone Tissue ◽  
Bone Repair ◽  
Critical Size ◽  
Healing Process ◽  
Bone Defects ◽  
Calcium Phosphate Bone Cement ◽  
Rabbit Tibia

Introduction: A novel injectable calcium phosphate bone cement (osteopaste) has been developed. Its potential application in orthopaedics as a filler of bone defects has been studied. The biomaterial was composed of tetra-calcium phosphate (TTCP) and tricalcium phosphate (TCP) powder. The aim of the present study was to evaluate the healing process of osteopaste in rabbit tibia. Materials and method: The implantation procedure was carried out on thirty-nine of New Zealand white rabbits. The in vivo bone formation was investigated by either implanting the Osteopaste, Jectos or MIIG – X3 into a critical size defect (CSD) model in the proximal tibial metaphysis. CSD without treatment served as negative control. After 1 day, 6 and 12 weeks, the rabbits were euthanized, the bone were harvested and subjected for analysis. Results: Radiological images and histological sections revealed integration of implants with bone tissue with no signs of graft rejection. There was direct contact between osteopaste material and host bone. The new bone was seen bridging the defect. Conclusion: The result showed that Osteopaste could be a new promising biomaterial for bone repair and has a potential in bone tissue engineering.

scholarly journals Genetically Engineered-MSC Therapies for Non-unions, Delayed Unions and Critical-size Bone Defects

2019 ◽  
Vol 20 (14) ◽  
pp. 3430 ◽  
Author(s):  
Jaime Freitas ◽  
Susana Gomes Santos ◽  
Raquel Madeira Gonçalves ◽  
José Henrique Teixeira ◽  
Mário Adolfo Barbosa ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Animal Studies ◽  
Bone Repair ◽  
Critical Size ◽  
Clinical Problem ◽  
Cell Types ◽  
Bone Defects ◽  
Genetically Engineered ◽  
Beneficial Effects

The normal bone regeneration process is a complex and coordinated series of events involving different cell types and molecules. However, this process is impaired in critical-size/large bone defects, with non-unions or delayed unions remaining a major clinical problem. Novel strategies are needed to aid the current therapeutic approaches. Mesenchymal stem/stromal cells (MSCs) are able to promote bone regeneration. Their beneficial effects can be improved by modulating the expression levels of specific genes with the purpose of stimulating MSC proliferation, osteogenic differentiation or their immunomodulatory capacity. In this context, the genetic engineering of MSCs is expected to further enhance their pro-regenerative properties and accelerate bone healing. Herein, we review the most promising molecular candidates (protein-coding and non-coding transcripts) and discuss the different methodologies to engineer and deliver MSCs, mainly focusing on in vivo animal studies. Considering the potential of the MSC secretome for bone repair, this topic has also been addressed. Furthermore, the promising results of clinical studies using MSC for bone regeneration are discussed. Finally, we debate the advantages and limitations of using MSCs, or genetically-engineered MSCs, and their potential as promoters of bone fracture regeneration/repair.

scholarly journals Osseous Healing in Surgically Prepared Bone Defects Using Different Grafting Materials: An Experimental Study in Pigs

2020 ◽  
Vol 8 (1) ◽  
pp. 7 ◽  
Author(s):  
Savvas Titsinides ◽  
Theodore Karatzas ◽  
Despoina Perrea ◽  
Efstathios Eleftheriadis ◽  
Leonidas Podaropoulos ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Repair ◽  
Healing Process ◽  
Bone Substitutes ◽  
Bone Defects ◽  
New Bone Formation ◽  
Post Surgery ◽  
Beta Tricalcium Phosphate ◽  
Close Proximity ◽  
Tissue Specimens

Regeneration of large jaw bone defects still remains a clinical challenge. To avoid incomplete bone repair, bone grafts have been advocated to support the healing process. This study comparatively evaluated new bone formation among a synthetic graft substitute, a human bone derivative, and a bovine xenograft. Materials were placed in 3 out of the 4 bone cavities, while 1 deficit was left empty, serving as a control, in mono-cortical defects, surgically prepared in the porcine calvaria bone. Animals were randomized in 2 groups and euthanized at 8 and 12 weeks. Harvested tissue specimens were qualitatively evaluated by histology. New bone formation was quantitatively measured by histomorphometry. Maximum new bone formation was noticed in defects grafted with beta-tricalcium phosphate b-TCP compared to the other bone substitutes, at 8 and 12 weeks post-surgery. Bovine and human allograft induced less new bone formation compared to empty bone cavity. Histologic analysis revealed that b-TCP was absorbed and substituted significantly, while bovine and human allograft was maintained almost intact in close proximity with new bone. Based on our findings, higher new bone formation was detected in defects filled with b-TCP when compared to bovine and human graft substitutes.

Bone repair using mineral trioxide aggregate combined to a material carrier, associated or not with calcium hydroxide in bone defects

Micron ◽  
2008 ◽  
Vol 39 (7) ◽  
pp. 868-874 ◽  
Author(s):  
Cássio do Nascimento ◽  
João Paulo Mardegan Issa ◽  
Mamie Mizusaki Iyomasa ◽  
Simone Cecílio Hallak Regalo ◽  
Selma Siéssere ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Bone Repair ◽  
Mineral Trioxide Aggregate ◽  
Bone Defects

Repairing of Osteochondral Defects in Joint Using Beta-TCP/ Carboxymethyl Chitin Composite

2005 ◽  
Vol 284-286 ◽  
pp. 791-794 ◽  
Author(s):  
Shingo Masuda ◽  
Yusuke Yoshihara ◽  
Kazuaki Muramatsu ◽  
Izumi Wakebe
Keyword(s):  
Mechanical Properties ◽  
Articular Cartilage ◽  
Tricalcium Phosphate ◽  
Bone Repair ◽  
Bone Defects ◽  
Promising Material ◽  
Osteochondral Defects ◽  
Repair Rate ◽  
Beta Tricalcium Phosphate

Beta-tricalcium phosphate/carboxymethyl chitin composites [TCP/CMCh] of various ratios of TCP granules and CMCh were made and their mechanical properties, handling properties and repair performance for bone defects and for osteochondral defects were investigated. Water pooling ratio of CMCh was approximately 40 times the weight itself. TCP/CMCh of a higher TCP ratio had higher stress at 50%-strain. The stress at 50%-strain of TCP/CMCh with 0, 2.5, 5.0, 7.5, 10 TCP ratios was 0.12, 0.51, 1.08, 1.46, 1.67 (MPa, n=5), respectively. The TCP/CMCh with 5.0 TCP ratios had the best total scores in handling tests. The bone repair rate of TCP/CMCh was TCP ratio 2.5< Blank= TCP ratio 7.5< TCP ratio 5.0. In the implantation test for osteochondral defects, TCP/CMCh was completely absorbed at four weeks after surgery. Regeneration of the articular cartilage was seen with TCP/CMCh and HA/CMCh but not with TCP granules, which remained eight weeks after implantation. The regenerated articular cartilage had remained 32 weeks after implantation. In conclusion, it was demonstrated that this TCP/CMCh composite was a promising material for repairing osteochondral defects.

scholarly journals Morphology of autogenous bone graft and castor oil polyurethane in the infraorbital rim of rabbits: a comparative study

2006 ◽  
Vol 21 (5) ◽  
pp. 341-347 ◽  
Author(s):  
José Carlos Garcia de Mendonça ◽  
Rafael De Rossi ◽  
Celso Massaschi Inouye ◽  
Diego Rodrigo Paulillo Bazan ◽  
João Carlos Castro Monteiro ◽  
...  
Keyword(s):  
Bone Graft ◽  
Castor Oil ◽  
Bone Repair ◽  
Bone Defects ◽  
Autogenous Bone Graft ◽  
Autogenous Bone ◽  
Control Group ◽  
Cell Nuclei ◽  
Zygomatic Bone ◽  
Chi Squared

PURPOSE: Morphological study comparing castor oil polyurethane and autogenous bone graft to repair bone defect in zygomatic bone of rabbits. METHODS: Twenty-four adult, male New Zealand rabbits were randomly distributed between two groups of twelve. Bone defects of 5mm in diameter were cut through the zygomatic bone and filled with polyurethane discs in the experimental group or autogenous bone harvested from the tibia in the control group. Animals were sacrificed after 30, 60 or 90 days, and the zygomatic bones were macro- and microscopically analyzed. Student's, Fisher's, chi-squared and McNemar's tests were used for statistical analysis. RESULTS: Both the castor oil polyurethane and the autograft adapted well to the defect, with no need for fixation. Fibrous connective tissue encapsulated the polyurethane, but no inflammation or giant cell reaction was observed. Acidophilic and basophilic areas were observed inside the micropores of the polyurethane, suggesting cell nuclei. After 90 days, bone repair with a lamellar pattern of organization was observed in the control group. CONCLUSION: The castor oil polyurethane was biocompatible and did not cause inflammation. It may be considered an alternative to fill bone defects.

scholarly journals Cellularizing hydrogel-based scaffolds to repair bone tissue: How to create a physiologically relevant micro-environment?

2017 ◽  
Vol 8 ◽  
pp. 204173141771207 ◽  
Author(s):  
Mathieu Maisani ◽  
Daniele Pezzoli ◽  
Olivier Chassande ◽  
Diego Mantovani
Keyword(s):  
Bone Regeneration ◽  
Bone Tissue ◽  
Bone Repair ◽  
Critical Issue ◽  
Bone Defects ◽  
Bone Tissue Regeneration ◽  
Differentiation Potential ◽  
Promising Alternative

Tissue engineering is a promising alternative to autografts or allografts for the regeneration of large bone defects. Cell-free biomaterials with different degrees of sophistication can be used for several therapeutic indications, to stimulate bone repair by the host tissue. However, when osteoprogenitors are not available in the damaged tissue, exogenous cells with an osteoblast differentiation potential must be provided. These cells should have the capacity to colonize the defect and to participate in the building of new bone tissue. To achieve this goal, cells must survive, remain in the defect site, eventually proliferate, and differentiate into mature osteoblasts. A critical issue for these engrafted cells is to be fed by oxygen and nutrients: the transient absence of a vascular network upon implantation is a major challenge for cells to survive in the site of implantation, and different strategies can be followed to promote cell survival under poor oxygen and nutrient supply and to promote rapid vascularization of the defect area. These strategies involve the use of scaffolds designed to create the appropriate micro-environment for cells to survive, proliferate, and differentiate in vitro and in vivo. Hydrogels are an eclectic class of materials that can be easily cellularized and provide effective, minimally invasive approaches to fill bone defects and favor bone tissue regeneration. Furthermore, by playing on their composition and processing, it is possible to obtain biocompatible systems with adequate chemical, biological, and mechanical properties. However, only a good combination of scaffold and cells, possibly with the aid of incorporated growth factors, can lead to successful results in bone regeneration. This review presents the strategies used to design cellularized hydrogel-based systems for bone regeneration, identifying the key parameters of the many different micro-environments created within hydrogels.

Evaluation of the Long-Term Results of Rat Cranial Bone Repair Using a Particular Xenograft

2010 ◽  
Vol 36 (3) ◽  
pp. 167-173 ◽  
Author(s):  
Hakan Develioglu ◽  
SerpilÜnver Saraydın ◽  
Ünal Kartal ◽  
Levent Taner
Keyword(s):  
Bone Formation ◽  
Bone Repair ◽  
Fibrous Tissue ◽  
Bone Defects ◽  
Parietal Bone ◽  
Control Area ◽  
Cranial Bone ◽  
Long Term Results ◽  
Tissue Samples ◽  
Defect Sites

Abstract Bone defects that cannot be healed completely are termed critical-sized defects and can be used to test bone grafts for medicine, dentistry, and periodontology. The aim of the present study was to detect the effects of a xenograft (Unilab Surgibone) on bone building in experimentally created parietal bone defects in rats. Standardized parietal bone defects were created in 16 rats, and each defect had a circular morphology 6 mm in diameter. The right defect sites were filled with porous particle material, and the left site was used as control. After the 3rd, 6th, and 12th months, rats were killed and tissue samples obtained from the related site of the cranium. Subsequently, histological sections were taken and stained with different stains for evaluation under light microscope. The rate of bone formation was assessed using a semiquantitative method. These results showed that dense collagenous tissue was observed in the control area during the third month, whereas xenograft particles were surrounded by a fibrous tissue layer at the implantation site. Osteoclast-like cells were also observed. There was also no significant bone repair at other observation periods. It can be concluded that the material used had no evidence of resorption and does not enhance bone formation. However, it seems biocompatible, osteoconductive, and could be used in a limited manner as a material for filling osseous defects in clinical practice.

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