scholarly journals Discrimination between sialic acid linkage modes using sialyllactose-imprinted polymers

RSC Advances ◽  
2021 ◽  
Vol 11 (36) ◽  
pp. 22409-22418
Author(s):  
Liliia Mavliutova ◽  
Bruna Munoz Aldeguer ◽  
Jesper Wiklander ◽  
Celina Wierzbicka ◽  
Chau Minh Huynh ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Imprinted Polymers ◽  
Sialic Acid Linkage

2,6′- and 2,3′-sialyllactose imprinted polymers (MIPs) capable of discriminating between two modes of sialic acid linkages in glycans are reported.

Systematic Optimisation of Microtiter Plate Lectin Assay to Improve Sialic Acid Linkage Detection

2021 ◽  
Vol 24 ◽  
Author(s):  
Nur Hanina Izzati Khairol Mokhtar ◽  
Ainulkhir Hussin ◽  
Aidil Abdul Hamid ◽  
Shahrul Hisham Zainal Ariffin ◽  
Muhammad Ashraf Shahidan
Keyword(s):  
Sialic Acid ◽  
Specific Binding ◽  
Specific Antigen ◽  
Blocking Agent ◽  
Microtiter Plate ◽  
Tween 20 ◽  
Nonspecific Binding ◽  
Binding Assays ◽  
High Background ◽  
Sialic Acid Linkage

Aims: We aimed to develop a high-throughput lectin assay with minimized background signals to investigate the interactions of lectins and sialic acid glycans, focusing on prostate-specific antigen (PSA). Background: High background signals resulting from nonspecific binding are a significant concern for microtiter plate-based enzyme-linked lectin sorbent assays (ELLSAs), as they can mask specific binding signals and cause false-positive results. Methods: In this study, we constructed an ELLSA based on different washing step parameters, including the number of washing cycles, NaCl and Tween-20 concentrations, and the type of blocking agent and evaluated the effects on both specific and nonspecific binding signals. Furthermore, we performed a PSA binding assay using the optimized ELLSA. Results: The optimal washing parameters based on the highest specific binding signal proposed four cycles of washing steps using a washing buffer containing a high salt concentration (0.5 M NaCl) and mild detergent (0.05% Tween-20). The utilization of the optimized washing parameters in this assay was shown to be sufficient to obtain the optimal binding signals without the use of any blocking agent. Binding assays performed using the optimized ELLSA revealed that the glycan of the PSA sample used in this study mainly consists of terminal α2,6-linked sialic acid, as strongly recognized by Sambucus nigra agglutinin (SNA) with a KD value of 12.38 nM. Conclusion: The ELLSA reported in this study provides a simple yet sensitive assay for sialic acid linkage recognition.

scholarly journals Evaluation of the Impact of Imprinted Polymer Particles on Morphology and Motility of Breast Cancer Cells by Using Digital Holographic Cytometry

2020 ◽  
Vol 10 (3) ◽  
pp. 750 ◽  
Author(s):  
Megha Patel ◽  
Marek Feith ◽  
Birgit Janicke ◽  
Kersti Alm ◽  
Zahra El-Schich
Keyword(s):  
Breast Cancer ◽  
Sialic Acid ◽  
Cancer Cells ◽  
Cell Lines ◽  
Cell Morphology ◽  
Molecularly Imprinted Polymers ◽  
Breast Cancer Cells ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Mcf 7

Breast cancer is the second most common cancer type worldwide and breast cancer metastasis accounts for the majority of breast cancer-related deaths. Tumour cells produce increased levels of sialic acid (SA) that terminates the monosaccharide on glycan chains of the glycosylated proteins. SA can contribute to cellular recognition, cancer invasiveness and increase the metastatic potential of cancer cells. SA-templated molecularly imprinted polymers (MIPs) have been proposed as promising reporters for specific targeting of cancer cells when deployed in nanoparticle format. The sialic acid-molecularly imprinted polymers (SA-MIPs), which use SA for the generation of binding sites through which the nanoparticles can target and stain breast cancer cells, opens new strategies for efficient diagnostic tools. This study aims at monitoring the effects of SA-MIPs on morphology and motility of the epithelial type MCF-7 and the highly metastatic MDAMB231 breast cancer cell lines, using digital holographic cytometry (DHC). DHC is a label-free technique that is used in cell morphology studies of e.g., cell volume, area and thickness as well as in motility studies. Here, we show that MCF-7 cells move slower than MDAMB231 cells. We also show that SA-MIPs have an effect on cell morphology, motility and viability of both cell lines. In conclusion, by using DH microscopy, we could detect SA-MIPs impact on different breast cancer cells regarding morphology and motility.

Optimization of O-GIG for O-Glycopeptide Characterization with Sialic Acid Linkage Determination

2020 ◽  
Vol 92 (16) ◽  
pp. 10946-10951
Author(s):  
Shuang Yang ◽  
Wells W. Wu ◽  
Rongfong Shen ◽  
Jonathan Sjogren ◽  
Lisa Parsons ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Sialic Acid Linkage

Potentiometric analysis of sialic acid with a flexible carbon cloth based on boronate affinity and molecularly imprinted polymers

The Analyst ◽  
2019 ◽  
Vol 144 (21) ◽  
pp. 6432-6437 ◽  
Author(s):  
Yanli Zhou ◽  
Huijie Huangfu ◽  
Jie Yang ◽  
Hui Dong ◽  
Lantao liu ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Molecularly Imprinted Polymers ◽  
Boronic Acid ◽  
Potentiometric Sensor ◽  
Carbon Cloth ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Potentiometric Analysis

A potentiometric sensor for sialic acid detection was designed based on a boronic acid-containing MIP modified carbon cloth electrode.

scholarly journals Different expression levels of glycans on leukemic cells—a novel screening method with molecularly imprinted polymers (MIP) targeting sialic acid

Tumor Biology ◽  
2016 ◽  
Vol 37 (10) ◽  
pp. 13763-13768 ◽  
Author(s):  
Zahra El-Schich ◽  
Mohammad Abdullah ◽  
Sudhirkumar Shinde ◽  
Nishtman Dizeyi ◽  
Anders Rosén ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Molecularly Imprinted Polymers ◽  
Screening Method ◽  
Leukemic Cells ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Expression Levels

scholarly journals Correction to Optimization of O-GIG for O-Glycopeptide Characterization with Sialic Acid Linkage Determination

2020 ◽  
Vol 92 (19) ◽  
pp. 13611-13611
Author(s):  
Shuang Yang ◽  
Wells W. Wu ◽  
Rongfong Shen ◽  
Lisa Parsons ◽  
John F. Cipollo
Keyword(s):  
Sialic Acid ◽  
Sialic Acid Linkage

scholarly journals Determination of sialic acid in serum samples by dispersive solid-phase extraction based on boronate-affinity magnetic hollow molecularly imprinted polymer sorbent

RSC Advances ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 5394-5401 ◽  
Author(s):  
Wei Huang ◽  
Xingyu Hou ◽  
Yukui Tong ◽  
Miaomiao Tian
Keyword(s):  
Sialic Acid ◽  
Molecularly Imprinted Polymers ◽  
Solid Phase ◽  
Phase Extraction ◽  
Dispersive Solid Phase Extraction ◽  
Imprinted Polymer ◽  
Serum Samples ◽  
Molecularly Imprinted ◽  
Imprinted Polymers

Boronate-affinity magnetic hollow molecularly imprinted polymers were prepared with sialic acid (SA) as a template to selectively extract SA from serum samples coupled with HPLC-UV.

Regioselective Ester Hydrolysis of Sialic Acid Derivatives Catalyzed by Molecularly Imprinted Polymers

Heterocycles ◽  
2009 ◽  
Vol 78 (12) ◽  
pp. 2943
Author(s):  
Kiyoshi Ikeda ◽  
Takashi Katsumata ◽  
Yuji Sugiyama ◽  
Keisuke Miyamoto ◽  
Kiyoshi Tanaka ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Molecularly Imprinted Polymers ◽  
Ester Hydrolysis ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Hydrolysis Of
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