scholarly journals Improved liquid–liquid extraction by modified magnetic nanoparticles for the detection of eight drugs in human blood by HPLC-MS

RSC Advances ◽  
2021 ◽  
Vol 11 (32) ◽  
pp. 19874-19884
Author(s):  
Feiyu Yang ◽  
Ke Ma ◽  
Yu Cao ◽  
Chunfang Ni
Keyword(s):  
Fatty Acids ◽  
Magnetic Nanoparticles ◽  
Phosphatidic Acid ◽  
Blood Sample ◽  
Human Blood ◽  
High Performance ◽  
Liquid Extraction ◽  
Liquid Liquid Extraction ◽  
Modified Magnetic Nanoparticles ◽  
Mesoporous Structures

High-performance magnetic Fe3O4@TiO2, nanospheres with mesoporous structures was synthesized for cleanup phosphatidic acid and fatty acids in blood sample for 8 drugs test with HPLC-MS.

scholarly journals Intravascular Residence Time Determination for the Cyanide Antidote Dimethyl Trisulfide in Rat by Using Liquid-Liquid Extraction Coupled with High Performance Liquid Chromatography

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Deepthika De Silva ◽  
Steven Lee ◽  
Anna Duke ◽  
Siva Angalakurthi ◽  
Ching-En Chou ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Residence Time ◽  
Rat Model ◽  
High Performance ◽  
Liquid Extraction ◽  
Dimethyl Trisulfide ◽  
Liquid Liquid Extraction ◽  
Cyanide Antidote ◽  
Limit Of Quantitation

These studies represent the first report on the intravascular residence time determinations for the cyanide antidote dimethyl trisulfide (DMTS) in a rat model by using high performance liquid chromatography coupled with ultraviolet absorption spectroscopy (HPLC-UV). The newly developed sample preparation included liquid-liquid extraction by cyclohexanone. The calibration curves showed a linear response for DMTS concentrations between 0.010 and 0.30 mg/mL withR2= 0.9994. The limit of detection for DMTS via this extraction method was 0.010 mg/mL, and the limit of quantitation was 0.034 mg/mL. Thus this calibration curve provided a tool for determining DMTS in the range between 0.04 and 0.30 mg/mL. Rats were given 20 mg/kg DMTS dose (in 15% Polysorbate 80) intravenously, and blood samples were taken 15, 60, 90, 120, and 240 min after DMTS injections. The data points were plotted as DMTS concentration in RBCs versus time, and the intravascular residence time was determined graphically. The results indicated a half-life of 36 min in a rat model, suggesting that the circulation time is long enough to provide a reasonable time interval for cyanide antagonism.

scholarly journals COMPARISON OF THREE EXTRACTION METHODS OF ALLOPURINOL IN URIC ACID HERBAL MEDICINE WITH HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY QUANTIFICATION

2021 ◽  
pp. 141-147
Author(s):  
RIMADANI PRATIWI ◽  
RASPATI D. MULYANINGSIH ◽  
NYI M. SAPTARINI
Keyword(s):  
Herbal Medicine ◽  
High Performance ◽  
Solid Phase ◽  
Extraction Methods ◽  
Liquid Extraction ◽  
Filtering Method ◽  
Liquid Liquid Extraction ◽  
Accuracy And Precision ◽  
Relative Standard ◽  
Parameters Of Accuracy

Objective: This study was aimed to understand and determine the effectiveness of allopurinol extraction in herbal medicine from three extraction methods based on parameters of accuracy and precision. Methods: The study consisted of three methods including dissolving and filtering, liquid-liquid extraction, and solid-phase extraction with mixed-mode cation exchanger (SPE-MCX). The procedures were carried out using NaOH and HCl in dissolving and filtering method; methanol, HCl, and ethyl acetate in liquid-liquid extraction; and NH4OH elution solvent in SPE-MCX. Results: The results showed that extraction effectiveness based on accuracy level was the dissolving and filtering method>SPE-MCX>liquid-liquid extraction with % recovery+SD of 91.314+2.903%, 87.533+4.950%, and 54.549+3.517%, respectively. The precision level was the dissolution and filtering method>SPE-MCX>liquid-liquid extraction based on % relative standard deviations (RSD) of 3.18%, 5.226%, and 6.446%, respectively. Conclusion: It can be concluded that the allopurinol extraction method with the highest effectiveness based on accuracy and precision parameters in herbal medicine is the dissolving and filtering method.

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