Enzymatic synthesis of novel fructosylated compounds by Ffase from Schwanniomyces occidentalis in green solvents

Understanding the miscibility behavior of ionic liquid (IL) / monomer, IL / polymer and IL / nanoparticle mixtures is critical for the use of ILs as green solvents in polymerization processes, and to rationalize recent observations concerning the superior solubility of some proteins in ILs when compared to standard solvents. In this work, the most relevant results obtained in terms of a three-component Flory-Huggins theory concerning the “Extra Solvent Power, ESP” of ILs when compared to traditional non-ionic solvents for monomeric solutes (case I), linear polymers (case II) and globular nanoparticles (case III) are presented. Moreover, useful ESP maps are drawn for the first time for IL mixtures corresponding to case I, II and III. Finally, a potential pathway to improve the miscibility of non-ionic polymers in ILs is also proposed.

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A common precursor to both prebiotic and biological pathways to RNA nucleotides

10.26434/chemrxiv.5519041.v3 ◽

2018 ◽

Author(s):

Andrea Pérez-Villa ◽

Thomas Georgelin ◽

Jean-François Lambert ◽

Marie-Christine Maurel ◽

François Guyot ◽

...

Keyword(s):

Enzymatic Synthesis ◽

De Novo ◽

Life Forms ◽

Hydrothermal Conditions ◽

Modern Life ◽

Ab Initio Simulations ◽

Spontaneous Formation ◽

Common Precursor ◽

Open Issue ◽

Anomeric Carbon

Understanding the mechanism of spontaneous formation of ribonucleotides under realistic prebiotic conditions is a key open issue of origins-of-life research. In cells, <i>de novo</i> and salvage nucleotide enzymatic synthesis combines 5-phospho-α -D-ribose-1-diphosphate ( α-PRPP) and nucleobases. Interestingly, these reactants are also known as prebiotically plausible compounds. Combining ab initio simulations with mass spectrometry experiments, we compellingly demonstrate that nucleobases and α -PRPP spontaneously combine, through the same facile mechanism, forming both purine and pyrimidine ribonucleotides, under mild hydrothermal conditions. Surprisingly, this mechanism is very similar to the biological one, and yields ribonucleotides with the same anomeric carbon chirality as in biological systems. These results suggest that natural selection might have optimized – through enzymes – a pre-existing ribonucleotide formation mechanism, carrying it forward to modern life forms.

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A common precursor to both prebiotic and biological pathways to RNA nucleotides

10.26434/chemrxiv.5519041.v2 ◽

2017 ◽

Author(s):

Andrea Pérez-Villa ◽

Thomas Georgelin ◽

Jean-François Lambert ◽

Marie-Christine Maurel ◽

François Guyot ◽

...

Keyword(s):

Enzymatic Synthesis ◽

De Novo ◽

Life Forms ◽

Hydrothermal Conditions ◽

Modern Life ◽

Ab Initio Simulations ◽

Spontaneous Formation ◽

Common Precursor ◽

Open Issue ◽

Anomeric Carbon

Understanding the mechanism of spontaneous formation of ribonucleotides under realistic prebiotic conditions is a key open issue of origins-of-life research. In cells, <i>de novo</i> and salvage nucleotide enzymatic synthesis combines 5-phospho-α -D-ribose-1-diphosphate ( α-PRPP) and nucleobases. Interestingly, these reactants are also known as prebiotically plausible compounds. Combining ab initio simulations with mass spectrometry experiments, we compellingly demonstrate that nucleobases and α -PRPP spontaneously combine, through the same facile mechanism, forming both purine and pyrimidine ribonucleotides, under mild hydrothermal conditions. Surprisingly, this mechanism is very similar to the biological one, and yields ribonucleotides with the same anomeric carbon chirality as in biological systems. These results suggest that natural selection might have optimized – through enzymes – a pre-existing ribonucleotide formation mechanism, carrying it forward to modern life forms.

Download Full-text