In situ monitoring of PISA morphologies

2021 ◽  
Author(s):  
Julia Y. Rho ◽  
Georg M. Scheutz ◽  
Satu Häkkinen ◽  
John B. Garrison ◽  
Qiao Song ◽  
...  

Fluorescent spectroscopy is a convenient method for monitoring the progression of polymerization-induced self-assembly (PISA).

Langmuir ◽  
2006 ◽  
Vol 22 (3) ◽  
pp. 897-900 ◽  
Author(s):  
Yaw Koon Koh ◽  
Chee Cheong Wong

2020 ◽  
Vol 7 (22) ◽  
pp. 2000848
Author(s):  
Elena Yunda ◽  
Fabienne Quilès ◽  
David Horwat ◽  
Raúl Gago ◽  
Jaafar Ghanbaja ◽  
...  

2004 ◽  
Vol 271 (1) ◽  
pp. 249-253 ◽  
Author(s):  
Zhi-mei Qi ◽  
Naoki Matsuda ◽  
Jose Santos ◽  
Takamitsu Yoshida ◽  
Akiko Takatsu ◽  
...  

2020 ◽  
Vol 834 ◽  
pp. 155139 ◽  
Author(s):  
Zhu Lu ◽  
Jun Dong ◽  
Qingyan Han ◽  
Wei Gao ◽  
Yongkai Wang ◽  
...  

2013 ◽  
Vol 214 (18) ◽  
pp. 2115-2123 ◽  
Author(s):  
Jheng-Guang Li ◽  
Wei-Cheng Chu ◽  
U.-Ser Jeng ◽  
Shiao-Wei Kuo

Author(s):  
D. Reis ◽  
B. Vian ◽  
J. C. Roland

Wall morphogenesis in higher plants is a problem still open to controversy. Until now the possibility of a transmembrane control and the involvement of microtubules were mostly envisaged. Self-assembly processes have been observed in the case of walls of Chlamydomonas and bacteria. Spontaneous gelling interactions between xanthan and galactomannan from Ceratonia have been analyzed very recently. The present work provides indications that some processes of spontaneous aggregation could occur in higher plants during the formation and expansion of cell wall.Observations were performed on hypocotyl of mung bean (Phaseolus aureus) for which growth characteristics and wall composition have been previously defined.In situ, the walls of actively growing cells (primary walls) show an ordered three-dimensional organization (fig. 1). The wall is typically polylamellate with multifibrillar layers alternately transverse and longitudinal. Between these layers intermediate strata exist in which the orientation of microfibrils progressively rotates. Thus a progressive change in the morphogenetic activity occurs.


Author(s):  
Dean A. Handley ◽  
Jack T. Alexander ◽  
Shu Chien

In situ preparation of cell cultures for ultrastructural investigations is a convenient method by which fixation, dehydration and embedment are carried out in the culture petri dish. The in situ method offers the advantage of preserving the native orientation of cell-cell interactions, junctional regions and overlapping configurations. In order to section after embedment, the petri dish is usually separated from the polymerized resin by either differential cryo-contraction or solvation in organic fluids. The remaining resin block must be re-embedded before sectioning. Although removal of the petri dish may not disrupt the native cellular geometry, it does sacrifice what is now recognized as an important characteristic of cell growth: cell-substratum molecular interactions. To preserve the topographic cell-substratum relationship, we developed a simple method of tapered rotary beveling to reduce the petri dish thickness to a dimension suitable for direct thin sectioning.


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