Constructing Firefly Luciferin Bioluminescence Probes for in Vivo Imaging

2022 ◽  
Author(s):  
Xingye Yang ◽  
Xiaojun Qin ◽  
Huimin Ji ◽  
Lupei Du ◽  
Minyong Li
Keyword(s):  
Real Time ◽  
In Vivo Imaging ◽  
Bioluminescence Imaging ◽  
Biological Systems ◽  
Firefly Luciferin ◽  
Visual Approach

Bioluminescence imaging (BLI) is a widely applied visual approach for real-time detecting many physiological and pathological processes in a variety of biological systems. Based on the caged strategy, lots of...

scholarly journals How to Select Firefly Luciferin Analogues for In Vivo Imaging

2021 ◽  
Vol 22 (4) ◽  
pp. 1848
Author(s):  
Ryohei Saito-Moriya ◽  
Jun Nakayama ◽  
Genta Kamiya ◽  
Nobuo Kitada ◽  
Rika Obata ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Life Science ◽  
Bioluminescence Imaging ◽  
High Sensitivity ◽  
The Novel ◽  
Current Review ◽  
Firefly Luciferin ◽  
In Vivo Bioluminescence Imaging ◽  
Luciferase Enzyme

Bioluminescence reactions are widely applied in optical in vivo imaging in the life science and medical fields. Such reactions produce light upon the oxidation of a luciferin (substrate) catalyzed by a luciferase (enzyme), and this bioluminescence enables the quantification of tumor cells and gene expression in animal models. Many researchers have developed single-color or multicolor bioluminescence systems based on artificial luciferin analogues and/or luciferase mutants, for application in vivo bioluminescence imaging (BLI). In the current review, we focus on the characteristics of firefly BLI technology and discuss the development of luciferin analogues for high-resolution in vivo BLI. In addition, we discuss the novel luciferin analogues TokeOni and seMpai, which show potential as high-sensitivity in vivo BLI reagents.

scholarly journals Multi-Modal Multi-Spectral Intravital Microscopic Imaging of Signaling Dynamics in Real-Time during Tumor–Immune Interactions

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 499
Author(s):  
Tracy W. Liu ◽  
Seth T. Gammon ◽  
David Piwnica-Worms
Keyword(s):  
Molecular Imaging ◽  
Single Cell ◽  
Real Time ◽  
Bioluminescence Imaging ◽  
Emission Spectra ◽  
Ccd Camera ◽  
Microscopic Imaging ◽  
Signaling Dynamics ◽  
Window Chamber

Intravital microscopic imaging (IVM) allows for the study of interactions between immune cells and tumor cells in a dynamic, physiologically relevant system in vivo. Current IVM strategies primarily use fluorescence imaging; however, with the advances in bioluminescence imaging and the development of new bioluminescent reporters with expanded emission spectra, the applications for bioluminescence are extending to single cell imaging. Herein, we describe a molecular imaging window chamber platform that uniquely combines both bioluminescent and fluorescent genetically encoded reporters, as well as exogenous reporters, providing a powerful multi-plex strategy to study molecular and cellular processes in real-time in intact living systems at single cell resolution all in one system. We demonstrate that our molecular imaging window chamber platform is capable of imaging signaling dynamics in real-time at cellular resolution during tumor progression. Importantly, we expand the utility of IVM by modifying an off-the-shelf commercial system with the addition of bioluminescence imaging achieved by the addition of a CCD camera and demonstrate high quality imaging within the reaches of any biology laboratory.

In vivo imaging of early stage apoptosis by measuring real-time caspase-3/7 activation

APOPTOSIS ◽  
2010 ◽  
Vol 16 (2) ◽  
pp. 198-207 ◽  
Author(s):  
Matteo Scabini ◽  
Fabio Stellari ◽  
Paolo Cappella ◽  
Sara Rizzitano ◽  
Gemma Texido ◽  
...  
Keyword(s):  
Real Time ◽  
In Vivo Imaging ◽  
Caspase 3 ◽  
Early Stage

2021 Frank Stinchfield Award: A novel cemented hip hemiarthroplasty infection model with real-time in vivo imaging in rats

2021 ◽  
Vol 103-B (7 Supple B) ◽  
pp. 9-16
Author(s):  
William J. Hadden ◽  
Mazen Ibrahim ◽  
Mariam Taha ◽  
Kerstin Ure ◽  
Yun Liu ◽  
...  
Keyword(s):  
Real Time ◽  
In Vivo Imaging ◽  
Biofilm Formation ◽  
Joint Infection ◽  
Volumetric Analysis ◽  
In Vivo Model ◽  
Infection Model ◽  
Sprague Dawley ◽  
Hip Hemiarthroplasty

Aims The aims of this study were to develop an in vivo model of periprosthetic joint infection (PJI) in cemented hip hemiarthroplasty, and to monitor infection and biofilm formation in real-time. Methods Sprague-Dawley rats underwent cemented hip hemiarthroplasty via the posterior approach with pre- and postoperative gait assessments. Infection with Staphylococcus aureus Xen36 was monitored with in vivo photoluminescent imaging in real-time. Pre- and postoperative gait analyses were performed and compared. Postmortem micro (m) CT was used to assess implant integration; field emission scanning electron microscopy (FE-SEM) was used to assess biofilm formation on prosthetic surfaces. Results All animals tolerated surgery well, with preservation of gait mechanics and weightbearing in control individuals. Postoperative in vivo imaging demonstrated predictable evolution of infection with logarithmic signal decay coinciding with abscess formation. Postmortem mCT qualitative volumetric analysis showed high contact area and both cement-bone and cement-implant interdigitation. FE-SEM revealed biofilm formation on the prosthetic head. Conclusion This study demonstrates the utility of a new, high-fidelity model of in vivo PJI using cemented hip hemiarthroplasty in rats. Inoculation with bioluminescent bacteria allows for non-invasive, real-time monitoring of infection. Cite this article: Bone Joint J 2021;103-B(7 Supple B):9–16.

Real-time in vivo bioluminescence imaging of drug-induced oxidative stress

2016 ◽  
Vol 258 ◽  
pp. S234
Author(s):  
S. Seyed Forootan ◽  
F. Mutter ◽  
J. Clarke ◽  
A. Kipar ◽  
K. Park ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Real Time ◽  
Bioluminescence Imaging ◽  
Drug Induced ◽  
In Vivo Bioluminescence Imaging

Real-time in vivo imaging of transgenic bioluminescent blood stages of rodent malaria parasites in mice

2006 ◽  
Vol 1 (1) ◽  
pp. 476-485 ◽  
Author(s):  
Blandine Franke-Fayard ◽  
Andrew P Waters ◽  
Chris J Janse
Keyword(s):  
Real Time ◽  
In Vivo Imaging ◽  
Malaria Parasites ◽  
Rodent Malaria

scholarly journals Real-time in vivo imaging of regional lung function in a mouse model of cystic fibrosis on a laboratory X-ray source

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Rhiannon P. Murrie ◽  
Freda Werdiger ◽  
Martin Donnelley ◽  
Yu-wei Lin ◽  
Richard P. Carnibella ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Lung Function ◽  
Mouse Model ◽  
Real Time ◽  
In Vivo Imaging ◽  
X Ray ◽  
Regional Lung Function ◽  
Regional Lung

Real-time in vivo imaging by high-speed spectral optical coherence tomography

2003 ◽  
Vol 28 (19) ◽  
pp. 1745 ◽  
Author(s):  
Maciej Wojtkowski ◽  
Tomasz Bajraszewski ◽  
Piotr Targowski ◽  
Andrzej Kowalczyk
Keyword(s):  
Optical Coherence Tomography ◽  
Real Time ◽  
In Vivo Imaging ◽  
High Speed ◽  
Optical Coherence ◽  
Spectral Optical Coherence Tomography

scholarly journals Real-Time In Vivo Imaging Reveals the Ability of Monocytes to Clear Vascular Amyloid Beta

Cell Reports ◽  
2013 ◽  
Vol 5 (3) ◽  
pp. 646-653 ◽  
Author(s):  
Jean-Philippe Michaud ◽  
Marc-André Bellavance ◽  
Paul Préfontaine ◽  
Serge Rivest
Keyword(s):  
Real Time ◽  
Amyloid Beta ◽  
In Vivo Imaging
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