1, 10/1, 11 Cyclization catalyzed by diverged plant sesquiterpene synthases is dependent on a single residue

2021 ◽  
Author(s):  
Xin Fang ◽  
Jin-Quan Huang ◽  
Dong-Mei Li ◽  
Jian-Xu Li ◽  
Jia-Ling Lin ◽  
...  
Keyword(s):  
Phylogenetic Analyses ◽  
Site Directed Mutagenesis ◽  
Terpene Synthase ◽  
Directed Mutagenesis

The exquisite chemodiversity of terpenoids are the product of the large diverse terpene synthase (TPS) superfamily. Here, by using structural and phylogenetic analyses and site-directed mutagenesis, we identified a residue...

Mechanistic Investigations on Microbial Type I Terpene Synthases through Site-directed Mutagenesis

Synthesis ◽  
2021 ◽  
Author(s):  
Houchao Xu ◽  
Jeroen Dickschat
Keyword(s):  
Crystal Structures ◽  
Site Directed Mutagenesis ◽  
Terpene Synthase ◽  
Directed Mutagenesis ◽  
Type I ◽  
Terpene Synthases ◽  
Conserved Motifs ◽  
Sequence Identity ◽  
The Past ◽  
Mechanistic Investigations

During the past three decades many terpene synthases have been characterised from all kingdoms of life. The type I of these enzymes from bacteria, fungi and protists commonly exhibit several highly conserved motifs and single residues, and the available crystal structures show a shared -helical fold, while the overall sequence identity is generally low. Several enzymes have been studied by site-directed mutagenesis, giving valuable insights into terpene synthase catalysis and the intriguing mechanisms of terpene synthases. Some mutants are also preparatively useful and give higher yields than the wildtype or a different product that is otherwise difficult to access. The accumulated knowledge obtained from these studies is presented and discussed in this review.

Isoishwarane synthase from Streptomyces lincolnensis

2021 ◽  
Author(s):  
Houchao Xu ◽  
Jan Rinkel ◽  
Jeroen S. Dickschat
Keyword(s):  
Natural Product ◽  
Enzyme Mechanism ◽  
Site Directed Mutagenesis ◽  
Terpene Synthase ◽  
Directed Mutagenesis ◽  
Isotopic Labelling ◽  
Streptomyces Lincolnensis ◽  
Isotopic Labelling Experiments

The product of a terpene synthase from Streptomyces lincolnensis has been identified as the new natural product isoishwarane. The enzyme mechanism was studied by isotopic labelling experiments and site-directed mutagenesis.

Genetic probes of ribosomal RNA function

1995 ◽  
Vol 73 (11-12) ◽  
pp. 859-868 ◽  
Author(s):  
Michael O'Connor ◽  
Carleen A. Brunelli ◽  
Matthew A. Firpo ◽  
Steven T. Gregory ◽  
Kathy R. Lieberman ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Ribosomal Rna ◽  
Phylogenetic Analyses ◽  
Random Mutagenesis ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Genetic Approach ◽  
Functional Roles ◽  
Genetic Probes ◽  
4.5S Rna

We have used a genetic approach to uncover the functional roles of rRNA in protein synthesis. Mutations were constructed in a cloned rrn operon by site-directed mutagenesis or isolated by genetic selections following random mutagenesis. We have identified mutations that affect each step in the process of translation. The data are consistent with the results of biochemical and phylogenetic analyses but, in addition, have provided novel information on regions of rRNA not previously investigated.Key words: decoding, peptidyltransferase, streptomycin, paromomycin, suppression, 4.5S RNA.

The site-directed mutagenesis and prokaryotic expression of midkine gene in Cynoglossus semilaevis

2013 ◽  
Vol 37 (3) ◽  
pp. 330
Author(s):  
Yanan WANG ◽  
Xudong LIU ◽  
Linlin MU ◽  
Zhipeng LIU ◽  
Chunmei LI ◽  
...  
Keyword(s):  
Prokaryotic Expression ◽  
Cynoglossus Semilaevis ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis

Site-directed mutagenesis on alkaline phosphatasefrompseudomonas aeruginosapao1 for enhanced activity

2016 ◽  
Vol 7 (4) ◽  
Author(s):  
UMA SELVARAJ ◽  
THIRUMALAI MUTHUKUMARESAN ◽  
GAYATHRI VIJAYENDRAN ◽  
SENTHIL KUMAR DEVAN ◽  
VENU BABU P ◽  
...  
Keyword(s):  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Enhanced Activity
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