A Drug-free Strategy to Combat Bacterial Infections with Magnetic Nanoparticles Biosynthesized in the Bacterial Pathogens

Nanoscale ◽  
2022 ◽  
Author(s):  
Deepa Ghosh ◽  
Swati Kaushik ◽  
Jijo Thomas ◽  
Vineeta Panwar ◽  
Preethi Murugesan ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Bacterial Infections ◽  
Bacterial Pathogens ◽  
Multiple Drug ◽  
Drug Resistant ◽  
Multiple Drug Resistant ◽  
Drug Free

The extensive and indiscriminate use of antibiotics in the ongoing COVID-19 pandemic might significantly contribute to the growing number of multiple drug resistant (MDR) bacteria. With the dwindling pipeline of...

scholarly journals A Simple Assay to Screen Antimicrobial Compounds Potentiating the Activity of Current Antibiotics

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Junaid Iqbal ◽  
Ruqaiyyah Siddiqui ◽  
Shahana Urooj Kazmi ◽  
Naveed Ahmed Khan
Keyword(s):  
Aqueous Extract ◽  
Bacterial Infections ◽  
Juglans Regia ◽  
Tree Bark ◽  
Resistant Bacteria ◽  
Multiple Drug ◽  
Drug Resistant ◽  
Antimicrobial Compounds ◽  
Drug Resistant Bacteria ◽  
Multiple Drug Resistant

Antibiotic resistance continues to pose a significant problem in the management of bacterial infections, despite advances in antimicrobial chemotherapy and supportive care. Here, we suggest a simple, inexpensive, and easy-to-perform assay to screen antimicrobial compounds from natural products or synthetic chemical libraries for their potential to work in tandem with the available antibiotics against multiple drug-resistant bacteria. The aqueous extract ofJuglans regiatree bark was tested against representative multiple drug-resistant bacteria in the aforementioned assay to determine whether it potentiates the activity of selected antibiotics. The aqueous extract ofJ. regiabark was added to Mueller-Hinton agar, followed by a lawn of multiple drug-resistant bacteria,Salmonella typhior enteropathogenicE. coli. Next, filter paper discs impregnated with different classes of antibiotics were placed on the agar surface. Bacteria incubated with extract or antibiotics alone were used as controls. The results showed a significant increase (>30%) in the zone of inhibition around the aztreonam, cefuroxime, and ampicillin discs compared with bacteria incubated with the antibiotics/extract alone. In conclusion, our assay is able to detect either synergistic or additive action ofJ. regiaextract against multiple drug-resistant bacteria when tested with a range of antibiotics.

scholarly journals Reduced Lytic Activity of Bacteriophages in Presence of Antibiotics Targeting Bacterial Protein Synthesis

2021 ◽  
Author(s):  
Medhavi Vashisth ◽  
Shikha Yashveer ◽  
Nitin Virmani ◽  
Bidhan Chandra Bera ◽  
Rajesh Kumar Vaid ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Bacterial Infections ◽  
Antagonistic Effect ◽  
Multiple Drug ◽  
Drug Resistant ◽  
Bacterial Protein ◽  
Protein Synthesis Machinery ◽  
Multiple Drug Resistant ◽  
Bacterial Protein Synthesis ◽  
Careful Assessment

Combination therapy of bacteriophage and antibiotics offers promise to treat multiple drug resistant bacterial infections through phage antibiotic synergy. However, its usage requires careful assessment as most antibiotics with mechanisms dependent upon inhibiting cell growth through interfering bacterial protein synthesis machinery were found to have an antagonistic effect on phage activity.

scholarly journals Mechanistic Insights into the Antimicrobial Actions of Metallic Nanoparticles and Their Implications for Multidrug Resistance

2019 ◽  
Vol 20 (10) ◽  
pp. 2468 ◽  
Author(s):  
Sibhghatulla Shaikh ◽  
Nazia Nazam ◽  
Syed Mohd Danish Rizvi ◽  
Khurshid Ahmad ◽  
Mohammad Hassan Baig ◽  
...  
Keyword(s):  
Metallic Nanoparticles ◽  
Bacterial Infections ◽  
Antibacterial Agents ◽  
Health Concern ◽  
Resistant Bacteria ◽  
Multiple Drug ◽  
Drug Resistant ◽  
Drug Resistant Bacteria ◽  
Multiple Drug Resistant ◽  
Bacterial Drug Resistance

Multiple drug-resistant bacteria are a severe and growing public health concern. Because relatively few antibiotics have been approved over recent years and because of the inability of existing antibiotics to combat bacterial infections fully, demand for unconventional biocides is intense. Metallic nanoparticles (NPs) offer a novel potential means of fighting bacteria. Although metallic NPs exert their effects through membrane protein damage, superoxide radicals and the generation of ions that interfere with the cell granules leading to the formation of condensed particles, their antimicrobial potential, and mechanisms of action are still debated. This article discusses the action of metallic NPs as antibacterial agents, their mechanism of action, and their effect on bacterial drug resistance. Based on encouraging data about the antibacterial effects of NP/antibiotic combinations, we propose that this concept be thoroughly researched to identify means of combating drug-resistant bacteria.

scholarly journals Isolation and Identification of Multiple Drug Resistant Bacterial Pathogens from Well Water Samples in and Around Wolaita Sodo Town, Southern Ethiopia

2021 ◽  
Vol 11 (3) ◽  
pp. 70-78
Author(s):  
Krishna Moorthy Sivalingam ◽  
Subramaniyan Vaithilingam
Keyword(s):  
Water Samples ◽  
Bacterial Pathogens ◽  
Multiple Drug ◽  
Well Water ◽  
Drug Resistant ◽  
Salmonella Spp ◽  
Multiple Drug Resistant ◽  
Enterococcus Spp ◽  
Shigella Spp ◽  
Wolaita Sodo

Water for human consumption is required to be free from any bacteria that might pose a health risk. The worldwide prevalence of pathogen contamination is a serious concern, and enhancing the understanding of major pathogen sources and their significant impacts on water resources is crucial. Further, these organisms underwent multiple drug resistance in their systems. The present study mainly focused on Multiple Drug Resistant (MDR) bacterial pathogens in well water system in and around Wolaita Sodo Town. A total of 380 well water samples were collected and screened for SPC, enrichment process and selective media isolation. Further all the isolates were used to know the sensitivity/resistant patterns by Bauer-Kirby method and based on resistant pattern MDR isolates were assessed and recorded. Amon 380 well water samples screened for water potability by SPC method, 154 samples were positive. Among 154 SPC positive water samples yielded 106 different isolates of bacteria and it includes five genera, namely Escherichia coli, Salmonella spp., Shigella spp., Proteus spp. and Enterococcus spp. All the isolated five genus were confirmed by preliminary characters, colony morphology and biochemical tests. Among 106 isolates, 13 isolates of E. coli, 3 isolates of Salmonella spp., 21 isolates of Shigella spp., 9 isolates of Proteus spp., and 4 isolates of Enterococcus spp. were documented as MDR bacterial pathogens. The high prevalence rate of multiple drug resistant bacterial pathogens in the well water samples could potentially pose a threat to people consuming this water. Therefore, the present study suggesting to monitor the quality of water and strict quality control measures should be put in place to ensure the effective treatment of drinking water. Keywords: Water samples, Standard Plate Count, Antibacterial Susceptibility Test and MDR

scholarly journals Isoalation of Multidrug Resistant Bacterial Pathogens from Human Hair Obtained from Barbing Salons Located within Benin City, Nigeria

2019 ◽  
pp. 1-7
Author(s):  
Lucky Evbuomwan ◽  
Patrick Emeka Chukwuka ◽  
Fortune Itojie Ebiala
Keyword(s):  
Escherichia Coli ◽  
Human Hair ◽  
Bacterial Pathogens ◽  
Multiple Drug ◽  
Microbiological Analysis ◽  
Drug Resistant ◽  
Proteus Vulgaris ◽  
Benin City ◽  
Multiple Drug Resistant ◽  
Corynebacterium Sp

This study was carried out to investigate the antibiotic susceptibility pattern of bacterial pathogens isolated from human hair in barbing salon. Hair samples were collected from ten different barbing saloons in Benin City and immediately transported to the laboratory for microbiological analysis using pour plate isolation method. Isolated bacteria were identified based on their cultural, morphological and biochemical characteristic. Antibiotics sensitivity was carried out using commercially available antibiotic disks. Total bacteria counts ranged from 2.80x104±0.8cfu/g to 6.13x104±0.21 cfu/g. Bacterial isolated included Escherichia coli, Proteus vulgaris, Streptococcus viridians and Corynebacterium sp. The least occurring bacteria were Escherichia coli and Proteus vulgaris with percentage distribution of 40% each while the most widely distributed was Corynebacterium sp. (80%). All the bacterial isolates were observed to be multiple drug resistant. The most effective drugs were sparfloxacin, perfloxacin, gentamicin, erythromycin and ciprofloxacin. This study has shown that high densities of multiple drug resistant pathogenic bacteria are usually associated with human hair.

Sign in / Sign up

Export Citation Format

Share Document