Assessing the single-molecule interactions between targeted peptides and the receptors on living cell membrane

Nanoscale ◽  
2021 ◽  
Author(s):  
Siying Li ◽  
Xuelei Pang ◽  
Jing Zhao ◽  
Qingrong Zhang ◽  
Yuping Shan
Keyword(s):  
Drug Delivery ◽  
Cell Membrane ◽  
Single Molecule ◽  
Living Cell ◽  
Drug Delivery Systems ◽  
Delivery Systems ◽  
Molecule Interactions

As one of potential ligands, targeted peptides have become an important part for construction intelligent drug delivery systems (DDSs). The targeting interaction of peptides with the receptors is a key...

scholarly journals Micelles Self-Assembled by 3-O-β-D-Glucopyranosyl Latycodigenin Enhance Cell Membrane Permeability, Promote Antibiotic Pulmonary Targeting and Improve Anti-infective Efficacy

2020 ◽  
Author(s):  
Man Zhang ◽  
Lili Ye ◽  
Hao Huang ◽  
Dandan Cheng ◽  
Kaixin Liu ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Cell Membrane ◽  
Membrane Permeability ◽  
Drug Delivery Systems ◽  
Delivery Systems ◽  
Pulmonary Drug Delivery ◽  
Cell Membrane Permeability ◽  
Bacterial Invasion ◽  
Lung Targeting ◽  
Self Assembled

Abstract Background: Nanoparticle-based pulmonary drug delivery systems are commonly developed and applied for drug-targeted delivery. It exhibits significant advantages compared to traditional pulmonary drug delivery systems. However, developing a formulation for each drug is a time-consuming and laborious task. Results: This study designed and constructed a universal lung-targeting nanoparticle. The self-assembled micelles were composed of a platycodon secondary saponin, 3-O-β-D-glucopyranosyl platycodigenin 682 (GP-682), via its specific amphiphilic structure. GP-682 micelles obtained a relatively stable zeta potential with a particle size between 60 to 90 nm, and the critical micelle concentration (CMC) value was approximately 42.3 μg/mL. Pre-incubation of GP-682 micelles markedly enhanced the cell membrane permeability, and improved drug uptake in vitro. The results were visualized using fluorescent dye tracing, transmission electron microscopy (TEM) observation and lactate dehydrogenase (LDH) releasing assay. The benefits enhanced the distribution of levofloxacin (Lev) in mouse lung tissue and reduced the overdosing of antibiotics. The acute lung injury mice model induced by Pseudomonas aeruginosa PA 14 strain demonstrated that pre-injection of GP-682 micelles before antibiotic administration produced a higher survival rate and anti-infective efficacy in vivo. It included a reduction in pulmonary injury, bacterial invasion and cytokines expression compared to treatment with Lev alone. Conclusions: GP-682 micelles are another nanoparticle-based pulmonary drug delivery system and provides a new option for lung-targeting therapy.

scholarly journals Micelles self-assembled by 3-O-β-D-glucopyranosyl latycodigenin enhance cell membrane permeability, promote antibiotic pulmonary targeting and improve anti-infective efficacy

2020 ◽  
Author(s):  
Man Zhang ◽  
Lili Ye ◽  
Hao Huang ◽  
Dandan Cheng ◽  
Kaixin Liu ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Cell Membrane ◽  
Membrane Permeability ◽  
Drug Delivery Systems ◽  
Delivery Systems ◽  
Pulmonary Drug Delivery ◽  
Cell Membrane Permeability ◽  
Bacterial Invasion ◽  
Lung Targeting ◽  
Self Assembled

Abstract Background: Nanoparticle-based pulmonary drug delivery systems are commonly developed and applied for drug-targeted delivery. They exhibit significant advantages compared to traditional pulmonary drug delivery systems. However, developing the formulation of each drug is a time-consuming and laborious task. Results: In this study, a universal lung-targeting nanoparticle was designed and constructed. The self-assembled micelles were composed of a platycodon secondary saponin, 3-O-β-D-glucopyranosyl platycodigenin 682 (GP-682), based on its specific amphiphilic structure. The GP-682 micelles exhibited a relatively stable zeta potential with a particle size between 60 and 90 nm, and the critical micelle concentration (CMC) value was approximately 42.3 μg/mL. Preincubation of GP-682 micelles markedly enhanced their cell membrane permeability and improved drug uptake in vitro. The results were visualized using fluorescent dye tracing, transmission electron microscopy (TEM) observations and the lactate dehydrogenase (LDH) release assay. The obtained benefits enhanced the distribution of levofloxacin (Lev) in mouse lung tissue and reduced antibiotics overdosing. The acute lung injury mouse model induced by the Pseudomonas aeruginosa PA 14 strain demonstrated that preinjection of GP-682 micelles before antibiotic administration resulted in a higher survival rate and anti-infective efficacy in vivo. It also caused reductions in pulmonary injury, bacterial invasion and cytokine expression compared with treatment with Lev alone. Conclusions: GP-682 micelles are another nanoparticle-based pulmonary drug delivery system and provide a new lung-targeting therapy option.

Nanostructured Silica Materials As Drug-Delivery Systems for Doxorubicin: Single Molecule and Cellular Studies

Nano Letters ◽  
2009 ◽  
Vol 9 (8) ◽  
pp. 2877-2883 ◽  
Author(s):  
Timo Lebold ◽  
Christophe Jung ◽  
Jens Michaelis ◽  
Christoph Bräuchle
Keyword(s):  
Drug Delivery ◽  
Single Molecule ◽  
Drug Delivery Systems ◽  
Delivery Systems ◽  
Silica Materials ◽  
Nanostructured Silica

scholarly journals Cell or Cell Membrane-Based Drug Delivery Systems

Theranostics ◽  
2015 ◽  
Vol 5 (8) ◽  
pp. 863-881 ◽  
Author(s):  
Songwei Tan ◽  
Tingting Wu ◽  
Dan Zhang ◽  
Zhiping Zhang
Keyword(s):  
Drug Delivery ◽  
Cell Membrane ◽  
Drug Delivery Systems ◽  
Delivery Systems

scholarly journals Micelles self-assembled by 3-O-β-D-glucopyranosyl latycodigenin enhances cell membrane permeability, promotes antibiotic pulmonary targeting and improves anti-infect efficacy

2020 ◽  
Author(s):  
Man Zhang ◽  
Lili Ye ◽  
Hao Huang ◽  
Dandan Cheng ◽  
Wenbo Wu ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Cell Membrane ◽  
Membrane Permeability ◽  
Drug Delivery Systems ◽  
Delivery Systems ◽  
Pulmonary Drug Delivery ◽  
Cell Membrane Permeability ◽  
Bacterial Invasion ◽  
Lung Targeting ◽  
Self Assembled

Abstract Background: Nanoparticle-based pulmonary drug delivery systems are commonly developed and applied for drug-targeted delivery. It exhibits significant advantages compared to traditional pulmonary drug delivery systems. However, developing a formulation for each drug is a time-consuming and laborious task. Results: The present study designed and constructed a universal lung-targeting nanoparticle. The self-assembled micelles were composed of a platycodon secondary saponin, 3-O-β-D-glucopyranosyl platycodigenin 682 (GP-682), via its specific amphiphilic structure. After optimization, the GP-682 micelles obtained a stable zeta potential with a particle size between 60 to 90 nm, and the CMC value was approximately 42.3 μg/mL. Pre-incubation of GP-682 micelles markedly enhanced the cell membrane permeability, and improved drug uptake in vitro . The results were visualized using fluorescent dye tracing, transmission electron microscopy observation and a lactate dehydrogenase releasing assay. The benefits enhanced the distribution of levofloxacin in mouse lung tissue and reduced the overdosing of antibiotic. Pseudomonas aeruginosa PA 14 strain-induced acute lung injury mice model demonstrated that preinjection of GP-682 micelles followed by antibiotic administration produced a higher survival rate and anti-infection efficacy in vivo , which included a reduction in pulmonary injury, bacterial invasion and the expression of cytokines compared to treatment with levofloxacin alone. Conclusions: GP-682 micelles are another nanoparticle-based pulmonary drug delivery system to increase the use of antibiotic and provide a new option for antibiotic resistance and lung-targeting therapy.

scholarly journals Micelles self-assembled by 3-O-β-d-glucopyranosyl latycodigenin enhance cell membrane permeability, promote antibiotic pulmonary targeting and improve anti-infective efficacy

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Man Zhang ◽  
Lili Ye ◽  
Hao Huang ◽  
Dandan Cheng ◽  
Kaixin Liu ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Cell Membrane ◽  
Membrane Permeability ◽  
Drug Delivery Systems ◽  
Delivery Systems ◽  
Pulmonary Drug Delivery ◽  
Cell Membrane Permeability ◽  
Bacterial Invasion ◽  
Lung Targeting ◽  
Self Assembled

Abstract Background Nanoparticle-based pulmonary drug delivery systems are commonly developed and applied for drug-targeted delivery. They exhibit significant advantages compared to traditional pulmonary drug delivery systems. However, developing the formulation of each drug is a time-consuming and laborious task. Results In this study, a universal lung-targeting nanoparticle was designed and constructed. The self-assembled micelles were composed of a platycodon secondary saponin, 3-O-β-d-glucopyranosyl platycodigenin 682 (GP-682), based on its specific amphiphilic structure. The GP-682 micelles exhibited a relatively stable zeta potential with a particle size between 60 and 90 nm, and the critical micelle concentration (CMC) value was approximately 42.3 μg/mL. Preincubation of GP-682 micelles markedly enhanced their cell membrane permeability and improved drug uptake in vitro. The results were visualized using fluorescent dye tracing, transmission electron microscopy (TEM) observations and the lactate dehydrogenase (LDH) release assay. The obtained benefits enhanced the distribution of levofloxacin (Lev) in mouse lung tissue and reduced antibiotics overdosing. The acute lung injury mouse model induced by the Pseudomonas aeruginosa PA 14 strain demonstrated that preinjection of GP-682 micelles before antibiotic administration resulted in a higher survival rate and anti-infective efficacy in vivo. It also caused reductions in pulmonary injury, bacterial invasion and cytokine expression compared with treatment with Lev alone. Conclusions GP-682 micelles are another nanoparticle-based pulmonary drug delivery system and provide a new lung-targeting therapy option.

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