In vivo Dual Fluorescence Imaging of Mucin 1 and Its Glycoform in Tumor Cells

Nanoscale ◽  
2021 ◽  
Author(s):  
xiaoli wang ◽  
Yufei Ye ◽  
Zhaohui Huang ◽  
Peter Seeberger ◽  
Jing Hu ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Fluorescence Imaging ◽  
Accurate Diagnosis ◽  
Biological Information ◽  
Dual Fluorescence ◽  
Protein Biomarkers ◽  
Mucin 1 ◽  
Efficient Approach

The most efficient approach for cancer identification and monitoring is the detection of cancer-associated protein biomarkers but an accurate diagnosis requires multiple analyses. Glycosylation profiling can provide important biological information...

scholarly journals In Vivo Dual Fluorescence Imaging to Detect Joint Destruction

2016 ◽  
Vol 40 (10) ◽  
pp. 1009-1013 ◽  
Author(s):  
Hongsik Cho ◽  
Fazal-Ur-Rehman Bhatti ◽  
Sangmin Lee ◽  
David D. Brand ◽  
Ae-Kyung Yi ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Joint Destruction ◽  
Dual Fluorescence

scholarly journals Fluorescence-guided stereotactic biopsy: a proof-of-concept study

2020 ◽  
Vol 132 (2) ◽  
pp. 530-536
Author(s):  
Robert Lynagh ◽  
Mark Ishak ◽  
Joseph Georges ◽  
Danielle Lopez ◽  
Hany Osman ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Fluorescence Imaging ◽  
Fluorescence Intensity ◽  
Stereotactic Biopsy ◽  
Fiber Optic ◽  
Normal Brain ◽  
Blue Fluorescence ◽  
Tumor Implantation

OBJECTIVEAccurate histopathological diagnoses are often necessary for treating neuro-oncology patients. However, stereotactic biopsy (STB), a common method for obtaining suspicious tissue from deep or eloquent brain regions, fails to yield diagnostic tissue in some cases. Failure to obtain diagnostic tissue can delay initiation of treatment and may result in further invasive procedures for patients. In this study, the authors sought to determine if the coupling of in vivo optical imaging with an STB system is an effective method for identification of diagnostic tissue at the time of biopsy.METHODSA minimally invasive fiber optic imaging system was developed by coupling a 0.65-mm-diameter coherent fiber optic fluorescence microendoscope to an STB system. Human U251 glioma cells were transduced for stable expression of blue fluorescent protein (BFP) to produce U251-BFP cells that were utilized for in vitro and in vivo experiments. In vitro, blue fluorescence was confirmed, and tumor cell delineation by fluorescein sodium (FNa) was quantified with fluorescence microscopy. In vivo, transgenic athymic rats implanted with U251-BFP cells (n = 4) were utilized for experiments. Five weeks postimplantation, the rats received 5–10 mg/kg intravenous FNa and underwent craniotomies overlying the tumor implantation site and contralateral normal brain. A clinical STB needle containing our 0.65-mm imaging fiber was passed through each craniotomy and images were collected. Fluorescence images from regions of interest ipsilateral and contralateral to tumor implantation were obtained and quantified.RESULTSLive-cell fluorescence imaging confirmed blue fluorescence from transduced tumor cells and revealed a strong correlation between tumor cells quantified by blue fluorescence and FNa contrast (R2 = 0.91, p < 0.001). Normalized to background, in vivo FNa-mediated fluorescence intensity was significantly greater from tumor regions, verified by blue fluorescence, compared to contralateral brain in all animals (301.7 ± 34.18 relative fluorescence units, p < 0.001). Fluorescence intensity measured from the tumor margin was not significantly greater than that from normal brain (p = 0.89). Biopsies obtained from regions of strong fluorescein contrast were histologically consistent with tumor.CONCLUSIONSThe authors found that in vivo fluorescence imaging with an STB needle containing a submillimeter-diameter fiber optic fluorescence microendoscope provided direct visualization of neoplastic tissue in an animal brain tumor model prior to biopsy. These results were confirmed in vivo with positive control cells and by post hoc histological assessment. In vivo fluorescence guidance may improve the diagnostic yield of stereotactic biopsies.

Activatable Near-Infrared Probe for Fluorescence Imaging of γ-Glutamyl Transpeptidase in Tumor Cells and In Vivo

2017 ◽  
Vol 23 (59) ◽  
pp. 14778-14785 ◽  
Author(s):  
Zhiliang Luo ◽  
Liandong Feng ◽  
Ruibing An ◽  
Guanfu Duan ◽  
Runqi Yan ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Glutamyl Transpeptidase

In Vivo Near-infrared Fluorescence Imaging of Carcinoembryonic Antigen–expressing Tumor Cells in Mice

Radiology ◽  
2008 ◽  
Vol 247 (3) ◽  
pp. 779-787 ◽  
Author(s):  
Marcus-René Lisy ◽  
Annika Goermar ◽  
Claudia Thomas ◽  
Jutta Pauli ◽  
Ute Resch-Genger ◽  
...  
Keyword(s):  
Carcinoembryonic Antigen ◽  
Tumor Cells ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Near Infrared Fluorescence ◽  
Near Infrared Fluorescence Imaging

A ZIF-90 nanoplatform loaded with an enzyme-responsive organic small-molecule probe for imaging the hypoxia status of tumor cells

Nanoscale ◽  
2020 ◽  
Vol 12 (27) ◽  
pp. 14870-14881
Author(s):  
Ruifen Zou ◽  
Qiuyu Gong ◽  
Zhenzhi Shi ◽  
Jianping Zheng ◽  
Jie Xing ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Fluorescence Imaging ◽  
Small Molecule ◽  
Imaging System ◽  
Small Molecule Probe

A new organic probe and a novel imaging system developed through loading the organic probe into ZIF-90@PEG were successfully synthesized for off–on fluorescence imaging via enzyme response to nitroreductase in vitro and in vivo.

scholarly journals Cover Feature: Activatable Near-Infrared Probe for Fluorescence Imaging of γ-Glutamyl Transpeptidase in Tumor Cells and In Vivo (Chem. Eur. J. 59/2017)

2017 ◽  
Vol 23 (59) ◽  
pp. 14667-14667 ◽  
Author(s):  
Zhiliang Luo ◽  
Liandong Feng ◽  
Ruibing An ◽  
Guanfu Duan ◽  
Runqi Yan ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Glutamyl Transpeptidase
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