A tetrahedral DNA nanorobot with conformational change in response to molecular trigger

Nanoscale ◽  
2021 ◽  
Author(s):  
Fengyu Liu ◽  
Xiaoming Liu ◽  
Qing Shi ◽  
Christopher Maffeo ◽  
Masaru Kojima ◽  
...  
Keyword(s):  
Conformational Change ◽  
Dna Origami ◽  
Physiological Conditions ◽  
Cargo Delivery ◽  
Nanoscale Sensing ◽  
External Stimuli

Dynamic DNA origami nanostructures that respond to external stimuli are promising platforms for cargo delivery and nanoscale sensing. However, the low stability of such nanostructures under physiological conditions presents a...

scholarly journals Dynamic DNA Origami Devices: from Strand-Displacement Reactions to External-Stimuli Responsive Systems

2018 ◽  
Vol 19 (7) ◽  
pp. 2114 ◽  
Author(s):  
Heini Ijäs ◽  
Sami Nummelin ◽  
Boxuan Shen ◽  
Mauri Kostiainen ◽  
Veikko Linko
Keyword(s):  
Dna Sequences ◽  
Rapid Evolution ◽  
Dna Nanotechnology ◽  
Dna Origami ◽  
Dna Assembly ◽  
Stimuli Responsive ◽  
Nanoscale Structures ◽  
Displacement Reactions ◽  
Materials Research ◽  
External Stimuli

DNA nanotechnology provides an excellent foundation for diverse nanoscale structures that can be used in various bioapplications and materials research. Among all existing DNA assembly techniques, DNA origami proves to be the most robust one for creating custom nanoshapes. Since its invention in 2006, building from the bottom up using DNA advanced drastically, and therefore, more and more complex DNA-based systems became accessible. So far, the vast majority of the demonstrated DNA origami frameworks are static by nature; however, there also exist dynamic DNA origami devices that are increasingly coming into view. In this review, we discuss DNA origami nanostructures that exhibit controlled translational or rotational movement when triggered by predefined DNA sequences, various molecular interactions, and/or external stimuli such as light, pH, temperature, and electromagnetic fields. The rapid evolution of such dynamic DNA origami tools will undoubtedly have a significant impact on molecular-scale precision measurements, targeted drug delivery and diagnostics; however, they can also play a role in the development of optical/plasmonic sensors, nanophotonic devices, and nanorobotics for numerous different tasks.

scholarly journals DNA Origami Nanomachines

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1766 ◽  
Author(s):  
Masayuki Endo ◽  
Hiroshi Sugiyama
Keyword(s):  
Dna Sequences ◽  
Molecular Motors ◽  
Molecular Machines ◽  
Dna Origami ◽  
Dna Nanostructures ◽  
Molecular Systems ◽  
Nanoscale Structures ◽  
Research Fields ◽  
Dna Strands ◽  
External Stimuli

DNA can assemble various molecules and nanomaterials in a programmed fashion and is a powerful tool in the nanotechnology and biology research fields. DNA also allows the construction of desired nanoscale structures via the design of DNA sequences. Structural nanotechnology, especially DNA origami, is widely used to design and create functionalized nanostructures and devices. In addition, DNA molecular machines have been created and are operated by specific DNA strands and external stimuli to perform linear, rotational, and reciprocating movements. Furthermore, complicated molecular systems have been created on DNA nanostructures by arranging multiple molecules and molecular machines precisely to mimic biological systems. Currently, DNA nanomachines, such as molecular motors, are operated on DNA nanostructures. Dynamic DNA nanostructures that have a mechanically controllable system have also been developed. In this review, we describe recent research on new DNA nanomachines and nanosystems that were built on designed DNA nanostructures.

Hydrogels as potential drug-delivery systems: network design and applications

2021 ◽  
Author(s):  
Hina Shoukat ◽  
Khuda Buksh ◽  
Sobia Noreen ◽  
Fahad Pervaiz ◽  
Irsah Maqbool
Keyword(s):  
Drug Delivery ◽  
Aqueous Solutions ◽  
Biomedical Applications ◽  
Drug Delivery Systems ◽  
Specific Drug ◽  
Potential Drug ◽  
Physiological Conditions ◽  
Crosslinked Polymer ◽  
External Stimuli ◽  
Hydrophilic Network

Hydrogels are 3D crosslinked polymer matrices having a colossal tendency to imbibe water and exhibit swelling under physiological conditions without deformation in their hydrophilic network. Hydrogels being biodegradable and biocompatible, gained consideration due to some unique characteristics: responsiveness to external stimuli (pH, temperature) and swelling in aqueous solutions. Hydrogels offer a promising option for various pharmaceutical and biomedical applications, including tissue-specific drug delivery at a predetermined, controlled rate. This article presents a brief review of the recent and fundamental advances to design hydrogels, the swelling and deswelling mechanism, various crosslinking methods and their use as an intelligent carrier in the pharmaceutical field. Recent applications of hydrogels are also briefly discussed and exemplified.

scholarly journals Non-enzymic activation of the covalent binding reaction of the complement protein C3

1983 ◽  
Vol 211 (2) ◽  
pp. 381-389 ◽  
Author(s):  
S K A Law
Keyword(s):  
Conformational Change ◽  
Hydroxy Group ◽  
Covalent Binding ◽  
Activation Mechanism ◽  
Complement Protein ◽  
Guanidinium Chloride ◽  
Cleavage Reaction ◽  
Binding Reaction ◽  
Physiological Conditions ◽  
Similar Dependence

The covalent binding of [3H]glycerol to C3 by the transfer of the acyl group of the internal thioester of C3 to the hydroxy group of glycerol can be activated either proteolytically by trypsin or by various chaotropes and denaturants. The activation of binding by trypsin or KBr showed similar dependence on the concentration of glycerol, indicating a similar activation mechanism. It is therefore concluded that the conformational change of the protein is the critical step in the binding reaction, and that the conversion of C3 into C3b under physiological conditions is only a means to induce the conformational change. Guanidinium chloride induces the binding of glycerol to C3 at concentrations of about 1 M. On increasing the concentration of guanidinium chloride the extent of binding declines and is accompanied by an increase in the autolytic cleavage reaction [Sim & Sim (1981) Biochem. J. 193, 129-141]. The autolytic cleavage reaction is therefore not independently activated with respect to the binding reaction. Its occurrence, however, is structurally restricted under physiological or limited denaturing conditions and is permissible only when C3 is brought to a higher denaturation state.

scholarly journals Cationic Albumin Encapsulated DNA Origami for Enhanced Cellular Transfection and Stability

Materials ◽  
2019 ◽  
Vol 12 (6) ◽  
pp. 949 ◽  
Author(s):  
Xuemei Xu ◽  
Shiqi Fang ◽  
Yuan Zhuang ◽  
Shanshan Wu ◽  
Qingling Pan ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Biomedical Applications ◽  
Transfection Efficiency ◽  
Dna Origami ◽  
Biological Applications ◽  
Dna Nanostructures ◽  
Cationic Protein ◽  
Physiological Conditions ◽  
Cellular Transfection ◽  
Biological Environment

DNA nanostructures, owing to their controllable and adaptable nature, have been considered as highly attractive nanoplatforms for biomedical applications in recent years. However, their use in the biological environment has been restricted by low cellular transfection efficiency in mammalian cells, weak stability under physiological conditions, and endonuclease degradation. Herein, we demonstrate an effective approach to facilitate fast transfection of DNA nanostructures and enhance their stability by encapsulating DNA origami with a biocompatible cationic protein (cHSA) via electrostatic interaction. The coated DNA origami is found to be stable under physiological conditions. Moreover, the cHSA coating could significantly improve the cellular transfection efficiency of DNA origami, which is essential for biological applications.

scholarly journals The Macrophage Iron Signature in Health and Disease

2021 ◽  
Vol 22 (16) ◽  
pp. 8457
Author(s):  
Christina Mertens ◽  
Oriana Marques ◽  
Natalie K. Horvat ◽  
Manuela Simonetti ◽  
Martina U. Muckenthaler ◽  
...  
Keyword(s):  
Broad Spectrum ◽  
Blood Cells ◽  
The Body ◽  
Tissue Homeostasis ◽  
Gene Profile ◽  
Physiological Conditions ◽  
Cellular Debris ◽  
Health And Disease ◽  
Fast Adaptation ◽  
External Stimuli

Throughout life, macrophages are located in every tissue of the body, where their main roles are to phagocytose cellular debris and recycle aging red blood cells. In the tissue niche, they promote homeostasis through trophic, regulatory, and repair functions by responding to internal and external stimuli. This in turn polarizes macrophages into a broad spectrum of functional activation states, also reflected in their iron-regulated gene profile. The fast adaptation to the environment in which they are located helps to maintain tissue homeostasis under physiological conditions.

A Study of the Mechanism of Binding between Neratinib and MAD2L1 Based on Molecular Simulation and Multi-spectroscopy Methods

2020 ◽  
Vol 25 (40) ◽  
pp. 4287-4295 ◽  
Author(s):  
Guangya Zhou ◽  
Manman Zhao ◽  
Ruirui Liang ◽  
Jiayang Xie ◽  
Xinyi Chen ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Conformational Change ◽  
Binding Sites ◽  
Biological Function ◽  
Kinase Inhibitor ◽  
Three Dimensional ◽  
Binding Mode ◽  
Physiological Conditions ◽  
Medicinal Value ◽  
Potential Binding

Background: Nilatinib is an irreversible tyrosine kinase inhibitor, which is used in the treatment of some kinds of cancer. To study the interaction between Neratinib and MAD2L1, a potential tumor target, is of guiding significance for enriching the medicinal value of Neratinib. Method: The binding mechanism between Mitotic arrest deficient 2-like protein 1 (MAD2L1) and Neratinib under simulative physiological conditions was investigated by molecule simulation and multi-spectroscopy approaches. Results: Molecular docking showed the most possible binding mode of Neratinib-MAD2L1 and the potential binding sites and interaction forces of the interaction between MAD2L1 and Neratinib. Fluorescence spectroscopy experiments manifested that Neratinib could interact with MAD2L1 and form a complex by hydrogen bond and van der Waals interaction. These results were consistent with the conclusions obtained from molecular docking. In addition, according to Synchronous fluorescence and three-dimensional fluorescence results, Neratinib might lead to the conformational change of MAD2L1, which may affect the biological functions of MAD2L1. Conclusion: This study indicated that Neratinib could interact with MAD2L1 and lead to the conformational change of MAD2L1. These works provide helpful insights for the further study of biological function of MAD2L1 and novel pharmacological utility of Neratinib.

scholarly journals Dynamic DNA Origami Devices

2018 ◽  
Author(s):  
Heini Ijäs ◽  
Sami Nummelin ◽  
Boxuan Shen ◽  
Mauri A. Kostiainen ◽  
Veikko Linko
Keyword(s):  
Rapid Evolution ◽  
Dna Nanotechnology ◽  
Dna Origami ◽  
Dna Assembly ◽  
Plasmonic Sensors ◽  
Nanophotonic Devices ◽  
Dna Strands ◽  
Materials Research ◽  
External Stimuli ◽  
Structural Dna Nanotechnology

Structural DNA nanotechnology provides an excellent foundation for diverse nanoscale shapes that can be used in various bioapplications and materials research. From all existing DNA assembly techniques, DNA origami has proven to be the most robust one for creating custom nanoshapes. Since its invention in 2006, building from the bottom up using DNA has drastically advanced, and therefore, more and more complex DNA-based systems have become accessible. So far, vast majority of the demonstrated DNA origami frameworks are static by nature, but interestingly, there also exist dynamic DNA origami devices that are increasingly coming into view. In this review, we discuss DNA origami nanostructures that perform controlled translational or rotational movement triggered by predefined DNA strands, various molecular interactions and/or other external stimuli such as light, pH, temperature and electromagnetic fields. The rapid evolution of such dynamic DNA origami tools will undoubtedly have a significant impact on molecular scale precision measurements, targeted drug delivery and diagnostics, but they can also play a role in development of optical/plasmonic sensors, nanophotonic devices and nanorobotics for numerous different tasks.

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