scholarly journals Imaging of nanoparticle uptake and kinetics of intracellular trafficking in individual cells

Nanoscale ◽  
2021 ◽  
Author(s):  
Natalia Vtyurina ◽  
Christoffer Åberg ◽  
Anna Salvati
Keyword(s):  
Intracellular Trafficking ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Drug Carriers ◽  
Live Cell ◽  
Nanoparticle Uptake ◽  
Kinetics Of

Live cell imaging is a powerful tool to understand how nano-sized objects, such as drug carriers used for nanomedicine applications, are taken up and trafficked by cells. Here we visualized...

scholarly journals Live Cell Imaging Of The Kinetics Of Ligand Binding At The Human Adenosine A3 Receptor

2009 ◽  
Vol 96 (3) ◽  
pp. 677a
Author(s):  
Lauren T. May ◽  
Stephen J. Briddon ◽  
Stephen J. Hill
Keyword(s):  
Ligand Binding ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Adenosine A3 Receptor ◽  
Kinetics Of

Live cell imaging of the endocytosis and the intracellular trafficking of multifunctional lipid nanoparticles

2006 ◽  
Author(s):  
Tieqiao Zhang ◽  
S. Narasimhan Danthi ◽  
Jianwu Xie ◽  
Dehong Hu ◽  
Peter Lu ◽  
...  
Keyword(s):  
Intracellular Trafficking ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Lipid Nanoparticles ◽  
Live Cell

scholarly journals Live cell imaging of duplex siRNA intracellular trafficking

2015 ◽  
Vol 43 (9) ◽  
pp. 4650-4660 ◽  
Author(s):  
Markus Hirsch ◽  
Mark Helm
Keyword(s):  
Intracellular Trafficking ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell

scholarly journals Torsin ATPases are required to complete nuclear pore complex biogenesis in interphase

2019 ◽  
Author(s):  
Anthony J. Rampello ◽  
Ethan Laudermilch ◽  
Nidhi Vishnoi ◽  
Sarah M. Prohet ◽  
Lin Shao ◽  
...  
Keyword(s):  
Nuclear Envelope ◽  
Nuclear Pore Complex ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Nuclear Pore ◽  
Ubiquitin Conjugation ◽  
Bleb Formation ◽  
Kinetics Of

AbstractNuclear envelope herniations (blebs) containing FG-nucleoporins and ubiquitin are the phenotypic hallmark of Torsin ATPase manipulation. Both the dynamics of blebbing and the connection to nuclear pore biogenesis remain poorly understood. We employ a proteomics-based approach to identify MLF2 as a luminal component of the bleb. Using an MLF2-based live cell imaging platform, we demonstrate that NE blebbing occurs rapidly and synchronously immediately after nuclear envelope reformation during mitosis. Bleb formation is independent of ubiquitin conjugation within the bleb, but strictly dependent on POM121, a transmembrane nucleoporin essential for interphase nuclear pore biogenesis. Nup358, a late marker for interphase nuclear pore complex (NPC) biogenesis, is underrepresented relative to FG nucleoporins in nuclear envelopes of Torsin-deficient cells. The kinetics of bleb formation, its dependence on POM121, and a reduction of mature NPCs in Torsin deficient cells lead us to conclude that the hallmark phenotype of Torsin manipulation represents the accumulation of stalled NPC assembly intermediates.

scholarly journals Torsin ATPase deficiency leads to defects in nuclear pore biogenesis and sequestration of MLF2

2020 ◽  
Vol 219 (6) ◽  
Author(s):  
Anthony J. Rampello ◽  
Ethan Laudermilch ◽  
Nidhi Vishnoi ◽  
Sarah M. Prophet ◽  
Lin Shao ◽  
...  
Keyword(s):  
Nuclear Envelope ◽  
Nuclear Pore Complex ◽  
Myeloid Leukemia ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Nuclear Pore ◽  
Ubiquitin Conjugation ◽  
Bleb Formation ◽  
Kinetics Of

Nuclear envelope herniations (blebs) containing FG-nucleoporins and ubiquitin are the phenotypic hallmark of Torsin ATPase manipulation. Both the dynamics of blebbing and the connection to nuclear pore biogenesis remain poorly understood. We employ a proteomics-based approach to identify myeloid leukemia factor 2 (MLF2) as a luminal component of the bleb. Using an MLF2-based live-cell imaging platform, we demonstrate that nuclear envelope blebbing occurs rapidly and synchronously immediately after nuclear envelope reformation during mitosis. Bleb formation is independent of ubiquitin conjugation within the bleb, but strictly dependent on POM121, a transmembrane nucleoporin essential for interphase nuclear pore biogenesis. Nup358, a late marker for interphase nuclear pore complex (NPC) biogenesis, is underrepresented relative to FG-nucleoporins in nuclear envelopes of Torsin-deficient cells. The kinetics of bleb formation, its dependence on POM121, and a reduction of mature NPCs in Torsin-deficient cells lead us to conclude that the hallmark phenotype of Torsin manipulation represents aberrant NPC intermediates.

Tuning Nanoparticle Uptake: Live-Cell Imaging Reveals Two Distinct Endocytosis Mechanisms Mediated by Natural and Artificial EGFR Targeting Ligand

Nano Letters ◽  
2012 ◽  
Vol 12 (7) ◽  
pp. 3417-3423 ◽  
Author(s):  
Frauke M. Mickler ◽  
Leonhard Möckl ◽  
Nadia Ruthardt ◽  
Manfred Ogris ◽  
Ernst Wagner ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Nanoparticle Uptake ◽  
Endocytosis Mechanisms
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