Poly(HPMA)–chlorambucil conjugate nanoparticles: facile fabrication and in vitro anti-cancer activity

2021 ◽  
Vol 45 (39) ◽  
pp. 18544-18551
Author(s):  
Guichen Li ◽  
Minzhi Zhao ◽  
Jia Zhang ◽  
Haining Li ◽  
Weibing Xu ◽  
...  
Keyword(s):  
Antitumor Effect ◽  
Anti Cancer ◽  
Facile Fabrication ◽  
Cancer Activity ◽  
Mcf 7

An acid-sensitive poly(HPMA)–Chl conjugate was developed and its antitumor effect towards HepG2 and MCF-7 cells was evaluated.

Synthetic NRG-1 functionalized DNA nanospindels towards HER2/neu targets for in vitro anti-cancer activity assessment against breast cancer MCF-7 cells

2020 ◽  
Vol 182 ◽  
pp. 113133 ◽  
Author(s):  
Mirza Muhammad Faran Ashraf Baig ◽  
Wing-Fu Lai ◽  
Reyaj Mikrani ◽  
Mehreen Jabeen ◽  
Muhammad Naveed ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Activity Assessment ◽  
Anti Cancer ◽  
Cancer Activity ◽  
Mcf 7

Preparation of wormlike polymeric nanoparticles coated with silica for delivery of methotrexate and evaluation of anticancer activity against MCF7 cells

2017 ◽  
Vol 31 (9) ◽  
pp. 1305-1316 ◽  
Author(s):  
Farhad Gharebaghi ◽  
Naser Dalali ◽  
Ebrahim Ahmadi ◽  
Hossein Danafar
Keyword(s):  
Polymeric Nanoparticles ◽  
Drug Loading ◽  
Free Drug ◽  
Spherical Nanoparticles ◽  
Mcf7 Cells ◽  
Anti Cancer ◽  
The Impact ◽  
Cancer Activity ◽  
Mcf 7

Methotrexate is one of the most effective drugs that is commonly used in the treatment of cancer. However, its application is limited due to low solubility, high toxicity and rapid metabolism. Therefore, in the present study, worm-like polymeric nanoparticles as carrier of methotrexate were prepared using biodegradable copolymers (mPEG–PCL). The impact of nanoparticles’ geometry on the loading, delivery and drug’s anti-cancer activity was investigated. The di-block copolymer mPEG–PCL was being synthesized by a ring opening polymerization of ɛ-caprolactone in the presence of mPEG as the initiator and Sn(oct)2 as the catalyst. It was used for the preparation of worm-like micelles and coated with silica, so that their structures are stable after drying. The synthesized copolymers and nanoparticles were characterized by FTIR, HNMR, GPC, XRD, TGA, DLS, and FE-SEM analyses. The efficiencies of drug loading and release of nanoparticles as in vitro, was studied by high performance liquid chromatography. The MTT method was used to estimate the toxicity on MCF-7 cell category. The obtained results showed that the nanoparticles were worm-like particles with less than 150 nm diameter and about 1 µm length. The loading and encapsulation efficiencies of drug by the worm-like nanoparticles were 3.5 ± 0.14% and 65.6 ± 0.12%, respectively, while they were obtained as 2.1 ± 0.08% and 26 ± 0.10%, respectively, for spherical nanoparticles. The methotrexate diffusional behavior of worm-like nanoparticles was compared with that of the spherical ones. On the other hand, the anti-cancer activity of MTX-loaded nanoparticles was more than the free drug. The results of the MTT assay showed strong and dose-dependent inhibition of cell (MCF-7 category) growth by the nanoparticles compared with MTX. The inhibitory concentrations (IC50 i.e. reduction viability of cell to 50%) obtained for worm-like, spherical nanoparticles and free drug (incubation times 72 h) were 8.25 ± 0.20, 9.15 ± 0.17, 12.28 ± 0.15 µg/mL, respectively. It can be concluded that application of non-spherical nanoparticles is a better and more effective strategy for controlled and slow release of methotrexate in the treatment of cancer.

scholarly journals In vitro Anti-oxidant and Anti-cancer Activity of Tetradesmus acuminatus Microalgae Extract on MCF-7 Human Breast Cancer Cell Line

2019 ◽  
Vol 16 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Mohammed Abdul Mujeeb ◽  
Ankalabasappa Vedamurthy ◽  
Arun Kashivishwanath Shettar ◽  
Sridevi Indrajeet Puranik ◽  
Shridhar Ghagane ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Cancer Cell Line ◽  
Human Breast Cancer Cell ◽  
Human Breast ◽  
Anti Cancer ◽  
Anti Oxidant ◽  
Cancer Activity ◽  
Mcf 7

scholarly journals GREEN SYNTHESIS AND CHARACTERIZATION OF ISOLATED FLAVONOID MEDIATED COPPER NANOPARTICLES BY USING THESPESIA POPULNEA LEAF EXTRACT AND ITS EVALUATION OF ANTI-OXIDANT AND ANTI-CANCER ACTIVITY

2022 ◽  
pp. 15-32
Author(s):  
MARIMUTHU GOKUL ◽  
UMARANI G. ◽  
AYYAMPERUMAL ESAKKI
Keyword(s):  
Green Synthesis ◽  
Cell Line ◽  
Reducing Power ◽  
Nano Particles ◽  
In Vivo Evaluation ◽  
Anti Cancer ◽  
Cancer Activity ◽  
Mcf 7

Objective: An Eco-friendly process of Green Synthesis of Copper Nano-particles (CuNPs) is an important aspect in the field of Nanotechnology using alternative feedstock, energy minimization, the design of less toxic and inherently safer chemicals. Methods: In this study, Copper Nano-Particles were synthesized by using isolated flavonoids to induce the reduction of Cu2+ions to CuNPs and also act as a capping and stabilizing agent. The solutions of CuSO4 and flavonoid were used as stock solutions for the preparation of CuNPs. Aqueous flavonoid(Quercetin) solution was mixed with CuSO4 solution separately. The reaction mixtures were immediately placed in a hot plate magnetic stirrer at 2000 rpm, 50-60 °C temperature and observed the change in colour of the solution from colourless to coloured solution. The synthesized CuNPs were characterized by various spectral methods for structural analysis of Nano-particles and In vitro and In vivo evaluation for anti-cancer activity. Results: The results described that the Copper Nano-particles are crystalline and amorphous with an average size of 295.4 nm and highly stable and having good hydrogen peroxide scavenging effect, reducing power and total antioxidant activity with the IC50value of 59.24μg/ml, 24.35μg/ml and16.83μg/ml respectively. The in vitro (HepG2 and MCF-7 cell lines) anti-cancer activity showing good results with IC50 values of 57.56 µg/ml for HepG2 cell line and 56.41 µg/ml for MCF-7 cell line. The in vivo anti-cancer activity also showing good results.

Design, Synthesis and In Vitro Anti-Cancer Evaluation of Novel Derivatives of 2-(2-Methyl-1,5-diaryl-1H-pyrrol-3-yl)-2-oxo-N-(pyridin-3- yl)acetamide

2020 ◽  
Vol 16 (3) ◽  
pp. 340-349
Author(s):  
Ebrahim S. Moghadam ◽  
Farhad Saravani ◽  
Ernest Hamel ◽  
Zahra Shahsavari ◽  
Mohsen Alipour ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Peripheral Neuropathy ◽  
Cell Line ◽  
Normal Cell ◽  
Caspase 3 ◽  
Annexin V ◽  
Normal Cell Line ◽  
Anti Cancer ◽  
Mcf 7

Objective: Several anti-tubulin agents were introduced for the cancer treatment so far. Despite successes in the treatment of cancer, these agents cause toxic side effects, including peripheral neuropathy. Comparing anti-tubulin agents, indibulin seemed to cause minimal peripheral neuropathy, but its poor aqueous solubility and other potential clinical problems have led to its remaining in a preclinical stage. Methods: Herein, indibulin analogues were synthesized and evaluated for their in vitro anti-cancer activity using MTT assay (on the MCF-7, T47-D, MDA-MB231 and NIH-3T3 cell lines), annexin V/PI staining assay, cell cycle analysis, anti-tubulin assay and caspase 3/7 activation assay. Results: One of the compounds, 4a, showed good anti-proliferative activity against MCF-7 cells (IC50: 7.5 μM) and low toxicity on a normal cell line (IC50 > 100 μM). All of the tested compounds showed lower cytotoxicity on normal cell line in comparison to reference compound, indibulin. In the annexin V/PI staining assay, induction of apoptosis in the MCF-7 cell line was observed. Cell cycle analysis illustrated an increasing proportion of cells in the sub-G-1 phase, consistent with an increasing proportion of apoptotic cells. No increase in G2/M cells was observed, consistent with the absence of anti-tubulin activity. A caspase 3/7 assay protocol showed that apoptosis induction by more potent compounds was due to activation of caspase 3. Conclusion: Newly synthesized compounds exerted acceptable anticancer activity and further investigation of current scaffold would be beneficial.

scholarly journals Doxorubicin loaded magnetism nanoparticles based on cyclodextrin dendritic-graphene oxide inhibited MCF-7 cell proliferation

2021 ◽  
Vol 12 (1) ◽  
pp. 8-15
Author(s):  
Ainaz Mihanfar ◽  
Niloufar Targhazeh ◽  
Shirin Sadighparvar ◽  
Saber Ghazizadeh Darband ◽  
Maryam Majidinia ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Anticancer Drug Delivery ◽  
Release Studies ◽  
Anti Cancer ◽  
Acidic Conditions ◽  
Mcf 7

Abstract Doxorubicin (DOX) is an effective chemotherapeutic agent used for the treatment of various types of cancer. However, its poor solubility, undesirable side effects, and short half-life have remained a challenge. We used a formulation based on graphene oxide as an anticancer drug delivery system for DOX in MCF-7 breast cancer cells, to address these issues. In vitro release studies confirmed that the synthesized formulation has an improved release profile in acidic conditions (similar to the tumor microenvironment). Further in vitro studies, including MTT, uptake, and apoptosis assays were performed. The toxic effects of the nanocarrier on the kidney, heart and liver of healthy rats were also evaluated. We observed that the DOX-loaded carrier improved the cytotoxic effect of DOX on the breast cell line compared to free DOX. In summary, our results introduce the DOX-loaded carrier as a potential platform for in vitro targeting of cancer cells and suggest further studies are necessary to investigate its in vivo anti-cancer potential.

scholarly journals Evaluation of the anti-cancer activity of the triterpenoidal saponin fraction isolated from the traditional Chinese medicine Conyza blinii H. Lév.

RSC Advances ◽  
2017 ◽  
Vol 7 (6) ◽  
pp. 3408-3412 ◽  
Author(s):  
Long Ma ◽  
Haiyan Liu ◽  
Lingpei Meng ◽  
Ping Qin ◽  
Botao Zhang ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Anti Cancer ◽  
Saponin Fraction ◽  
Cancer Activity

Triterpenoidal saponins fraction isolated from a traditional Chinese medicine Conyza blinii H. Lév. demonstrates anti-cancer activity both in vitro and in vivo.

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