Discovery of 3-chlorobenzyl linked 1,9-diazaspiro[5.5]undecane derivatives, a lead for Dengue virus type 2 infection

2021 ◽  
Author(s):  
Madhusudhana Reddy Gangireddy ◽  
Vishnu Nayak Badavath ◽  
Caroline Velez ◽  
Naphat Loeanurit ◽  
Abhishek Thakur ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Health Threat ◽  
Dengue Virus Type 2

Dengue virus poses a serious worldwide health threat with up to 400 million infections occurring annually in over 100 countries. Currently, there are no specific therapeutics available, and the only...

scholarly journals Reduced sphingosine kinase 1 activity in dengue virus type-2 infected cells can be mediated by the 3′ untranslated region of dengue virus type-2 RNA

2013 ◽  
Vol 94 (11) ◽  
pp. 2437-2448 ◽  
Author(s):  
J. M. Carr ◽  
T. Kua ◽  
J. N. Clarke ◽  
J. K Calvert ◽  
J. R. Zebol ◽  
...  
Keyword(s):  
Cell Death ◽  
Dengue Virus ◽  
Virus Type ◽  
Elongation Factor ◽  
Sphingosine Kinase ◽  
Sphingosine Kinase 1 ◽  
Diarrhea Virus ◽  
Infected Cells ◽  
Dengue Virus Type 2

Sphingosine kinase 1 (SphK1) is a lipid kinase with important roles including regulation of cell survival. We have previously shown reduced SphK1 activity in cells with an established dengue virus type-2 (DENV-2) infection. In this study, we examined the effect of alterations in SphK1 activity on DENV-2 replication and cell death and determined the mechanisms of the reduction in SphK1 activity. Chemical inhibition or overexpression of SphK1 after established DENV-2 infection had no effect on infectious DENV-2 production, although inhibition of SphK1 resulted in enhanced DENV-2-induced cell death. Reduced SphK1 activity was observed in multiple cell types, regardless of the ability of DENV-2 infection to be cytopathic, and was mediated by a post-translational mechanism. Unlike bovine viral diarrhea virus, where SphK1 activity is decreased by the NS3 protein, SphK1 activity was not affected by DENV-2 NS3 but, instead, was reduced by expression of the terminal 396 bases of the 3′ UTR of DENV-2 RNA. We have previously shown that eukaryotic elongation factor 1A (eEF1A) is a direct activator of SphK1 and here DENV-2 RNA co-localized and co-precipitated with eEF1A from infected cells. We propose that the reduction in SphK1 activity late in DENV-2-infected cells is a consequence of DENV-2 out-competing SphK1 for eEF1A binding and hijacking cellular eEF1A for its own replication strategy, rather than a specific host or virus-induced change in SphK1 to modulate viral replication. Nonetheless, reduced SphK1 activity may have important consequences for survival or death of the infected cell.

scholarly journals In VitroEvaluation of Antiprotozoal and Antiviral Activities of Extracts from ArgentineanMikaniaSpecies

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Laura C. Laurella ◽  
Fernanda M. Frank ◽  
Andrea Sarquiz ◽  
María R. Alonso ◽  
Gustavo Giberti ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Sesquiterpene Lactones ◽  
Infrared Analysis ◽  
Leishmania Braziliensis ◽  
Organic Extract ◽  
Chromatographic Profile ◽  
Antiviral Activities ◽  
Dengue Virus Type 2

The aim of this study was to investigate the antiprotozoal and antiviral activities of four ArgentineanMikaniaspecies. The organic and aqueous extracts ofMikania micrantha, M. parodii, M. periplocifolia,andM. cordifoliawere tested onTrypanosoma cruziepimastigotes,Leishmania braziliensispromastigotes, and dengue virus type 2. The organic extract ofM. micranthawas the most active againstT. cruziandL. braziliensisexhibiting a growth inhibition of77.6±4.5% and84.9±6.1%, respectively, at a concentration of 10 μg/ml. The bioguided fractionation ofM. micranthaorganic extract led to the identification of two active fractions. The chromatographic profile and infrared analysis of these fractions revealed the presence of sesquiterpene lactones. None of the tested extracts were active against dengue virus type 2.

scholarly journals Association between NS3 and NS5 Proteins of Dengue Virus Type 2 in the Putative RNA Replicase Is Linked to Differential Phosphorylation of NS5

1995 ◽  
Vol 270 (32) ◽  
pp. 19100-19106 ◽  
Author(s):  
Mini Kapoor ◽  
Luwen Zhang ◽  
Muralidhara Ramachandra ◽  
Jingo Kusukawa ◽  
Kurt E. Ebner ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Differential Phosphorylation ◽  
Dengue Virus Type 2

In Vitro Antiviral Activity of Pinus merkusii (Pinaceae) Stem Bark and Cone against Dengue Virus Type-2 (DENV-2)

2021 ◽  
pp. 3705-3708
Author(s):  
Arif Nur Muhammad Ansori ◽  
Amaq Fadholly ◽  
Annise Proboningrat ◽  
Suhailah Hayaza ◽  
Raden Joko Kuncoroningrat Susilo ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Dengue Virus ◽  
Virus Type ◽  
Stem Bark ◽  
Endemic Region ◽  
In Vivo Evaluation ◽  
African Green Monkey Kidney ◽  
Antiviral Compound ◽  
Dengue Virus Type 2

Dengue is a major mosquito-borne disease that currently has no effective antiviral or vaccine available. Recently, Indonesia is one of the largest countries in the dengue-endemic region, with a total population of more than 250 million. In the present study, the antiviral activity of P. merkusii stem bark and cone were evaluated against dengue virus type-2 (DENV-2; NCBI accession number: KT012509) isolated from Surabaya, Indonesia. We revealed that P. merkusii stem bark and cone inhibited DENV-2 in Vero cells (originally from African green monkey kidney) with IC50= 140.63 μg/mL and 73.78 μg/mL, CC50= 89.65 μg/mL and 249.5 μg/mL, SI= 0.64 and 3.38, respectively. The findings presented here suggest that P. merkusii stem bark and cone exerts potent antiviral activity against DENV-2. Hence, P. merkusii stem bark and cone are potent to inhibit DENV-2 and should be considered for in vivo evaluation in the development of an effective antiviral compound against DENV-2.

scholarly journals Antiviral actions of flavanoid-derived compounds on dengue virus type-2

2010 ◽  
pp. 294-302 ◽  
Author(s):  
Mudiana Muhamad ◽  
Lee Yean Kee ◽  
Noorsaadah Abd. Rahman ◽  
Rohana Yusof
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Dengue Virus Type 2

In-vitro effect of human cathelicidin antimicrobial peptide LL-37 on dengue virus type 2

Peptides ◽  
2017 ◽  
Vol 92 ◽  
pp. 23-30 ◽  
Author(s):  
K. Alagarasu ◽  
P.S. Patil ◽  
P. Shil ◽  
M. Seervi ◽  
M.B. Kakade ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Antimicrobial Peptide ◽  
Virus Type ◽  
Vitro Effect ◽  
Dengue Virus Type 2
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