Label-free electrochemical aptasensor based on reduced graphene oxide–hemin–chitosan nanocomposite for the determination of glypican-3

2021 ◽  
Author(s):  
Min Chen ◽  
Haimei Li ◽  
Xueming Su ◽  
Runqiang Wu ◽  
Huafu Feng ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Label Free ◽  
Electrochemical Aptasensor ◽  
Recognition Element ◽  
Reduced Graphene

A GPC3 electrochemical aptasensor was constructed using an RGO–H–CS-modified SPE as a biosensing platform and GPC3 aptamer as the recognition element.

scholarly journals A label-free biosensor based on graphene and reduced graphene oxide dual-layer for electrochemical determination of beta-amyloid biomarkers

2020 ◽  
Vol 187 (5) ◽  
Author(s):  
Jagriti Sethi ◽  
Michiel Van Bulck ◽  
Ahmed Suhail ◽  
Mina Safarzadeh ◽  
Ana Perez-Castillo ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Limit Of Detection ◽  
Electrochemical Techniques ◽  
Differential Pulse ◽  
Electrochemical Determination ◽  
Label Free ◽  
Reduced Graphene ◽  
Electrochemically Reduced Graphene Oxide

AbstractA label-free biosensor is developed for the determination of plasma-based Aβ1–42 biomarker in Alzheimer’s disease (AD). The platform is based on highly conductive dual-layer of graphene and electrochemically reduced graphene oxide (rGO). The modification of dual-layer with 1-pyrenebutyric acid N-hydroxysuccinimide ester (Pyr-NHS) is achieved to facilitate immobilization of H31L21 antibody. The effect of these modifications were studied with morphological, spectral and electrochemical techniques. The response of the biosensor was evaluated using differential pulse voltammetry (DPV). The data was acquired at a working potential of ~ 180 mV and a scan rate of 50 mV s−1. A low limit of detection (LOD) of 2.398 pM is achieved over a wide linear range from 11 pM to 55 nM. The biosensor exhibits excellent specificity over Aβ1–40 and ApoE ε4 interfering species. Thus, it provides a viable tool for electrochemical determination of Aβ1–42. Spiked human and mice plasmas were used for the successful validation of the sensing platform in bio-fluidic samples. The results obtained from mice plasma analysis concurred with the immunohistochemistry (IHC) and magnetic resonance imaging (MRI) data obtained from brain analysis.

scholarly journals Correction to: A label-free biosensor based on graphene and reduced graphene oxide dual-layer for electrochemical determination of beta-amyloid biomarkers

2020 ◽  
Vol 187 (6) ◽  
Author(s):  
Jagriti Sethi ◽  
Michiel Van Bulck ◽  
Ahmed Suhail ◽  
Mina Safarzadeh ◽  
Ana Perez-Castillo ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Beta Amyloid ◽  
Electrochemical Determination ◽  
Label Free ◽  
Reduced Graphene

A novel reduced graphene oxide/molybdenum disulfide/polyaniline nanocomposite-based electrochemical aptasensor for detection of aflatoxin B1

The Analyst ◽  
2018 ◽  
Vol 143 (7) ◽  
pp. 1644-1649 ◽  
Author(s):  
Girma Selale Geleta ◽  
Zhen Zhao ◽  
Zhenxin Wang
Keyword(s):  
Graphene Oxide ◽  
Molybdenum Disulfide ◽  
Reduced Graphene Oxide ◽  
Aflatoxin B1 ◽  
Label Free ◽  
Electrochemical Aptasensor ◽  
Reduced Graphene ◽  
Graphene Nanocomposite ◽  
Aflatoxin B ◽  
Polyaniline Nanocomposite

A label-free electrochemical aptasensor based on graphene nanocomposite was successfully fabricated for Aflatoxin B1 (AFB1) detection.

Sign in / Sign up

Export Citation Format

Share Document