scholarly journals Molecular dynamics study of the internalization of cell-penetrating peptides containing unnatural amino acids across membranes

2021 ◽  
Author(s):  
Joan Gimenez-Dejoz ◽  
Keiji Numata
Keyword(s):  
Molecular Dynamics ◽  
Amino Acids ◽  
Unnatural Amino Acids ◽  
Biological Membranes ◽  
Cell Penetrating Peptides ◽  
Delivery Systems ◽  
Cell Penetrating ◽  
Target Molecules

Peptide-based delivery systems that deliver target molecules into cells have been gaining traction. These systems need cell-penetrating peptides (CPPs), which have the remarkable ability to penetrate into biological membranes and...

Plasmid DNA delivery by arginine-rich cell-penetrating peptides containing unnatural amino acids

2016 ◽  
Vol 24 (12) ◽  
pp. 2681-2687 ◽  
Author(s):  
Takuma Kato ◽  
Hiroko Yamashita ◽  
Takashi Misawa ◽  
Koyo Nishida ◽  
Masaaki Kurihara ◽  
...  
Keyword(s):  
Amino Acids ◽  
Plasmid Dna ◽  
Unnatural Amino Acids ◽  
Dna Delivery ◽  
Cell Penetrating Peptides ◽  
Cell Penetrating

scholarly journals Cell-Penetrating Peptides: Emerging Tools for mRNA Delivery

Pharmaceutics ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 78
Author(s):  
Hidetomo Yokoo ◽  
Makoto Oba ◽  
Satoshi Uchida
Keyword(s):  
Amino Acids ◽  
Genetic Diseases ◽  
Cell Penetrating Peptides ◽  
Delivery Systems ◽  
Endosomal Escape ◽  
Messenger Rnas ◽  
Distinctive Features ◽  
Cell Penetrating ◽  
Preventive Vaccination ◽  
Natural Amino Acids

Messenger RNAs (mRNAs) were previously shown to have great potential for preventive vaccination against infectious diseases and therapeutic applications in the treatment of cancers and genetic diseases. Delivery systems for mRNAs, including lipid- and polymer-based carriers, are being developed for improving mRNA bioavailability. Among these systems, cell-penetrating peptides (CPPs) of 4–40 amino acids have emerged as powerful tools for mRNA delivery, which were originally developed to deliver membrane-impermeable drugs, peptides, proteins, and nucleic acids to cells and tissues. Various functionalities can be integrated into CPPs by tuning the composition and sequence of natural and non-natural amino acids for mRNA delivery. With the employment of CPPs, improved endosomal escape efficiencies, selective targeting of dendritic cells (DCs), modulation of endosomal pathways for efficient antigen presentation by DCs, and effective mRNA delivery to the lungs by dry powder inhalation have been reported; additionally, they have been found to prolong protein expression by intracellular stabilization of mRNA. This review highlights the distinctive features of CPP-based mRNA delivery systems.

scholarly journals Tumour lineage-homing cell-penetrating peptides as anticancer molecular delivery systems

2012 ◽  
Vol 3 (1) ◽  
Author(s):  
Eisaku Kondo ◽  
Ken Saito ◽  
Yuichi Tashiro ◽  
Kaeko Kamide ◽  
Shusei Uno ◽  
...  
Keyword(s):  
Cell Penetrating Peptides ◽  
Delivery Systems ◽  
Molecular Delivery ◽  
Cell Penetrating

Development of helix-stabilized cell-penetrating peptides containing cationic α,α-disubstituted amino acids as helical promoters

2017 ◽  
Vol 25 (6) ◽  
pp. 1846-1851 ◽  
Author(s):  
Hiroko Yamashita ◽  
Takashi Misawa ◽  
Makoto Oba ◽  
Masakazu Tanaka ◽  
Mikihiko Naito ◽  
...  
Keyword(s):  
Amino Acids ◽  
Cell Penetrating Peptides ◽  
Cell Penetrating ◽  
Disubstituted Amino Acids

Stereochemistry as a determining factor for the effect of a cell-penetrating peptide on cellular viability and epithelial integrity

2018 ◽  
Vol 475 (10) ◽  
pp. 1773-1788 ◽  
Author(s):  
Ditlev Birch ◽  
Malene V. Christensen ◽  
Dan Staerk ◽  
Henrik Franzyk ◽  
Hanne Mørck Nielsen
Keyword(s):  
Amino Acids ◽  
Cell Viability ◽  
Mammalian Cells ◽  
Membrane Integrity ◽  
Cell Penetrating Peptides ◽  
Proliferating Cells ◽  
Epithelial Integrity ◽  
Cell Penetrating ◽  
Culture Models ◽  
Well Differentiated

Cell-penetrating peptides (CPPs) comprise efficient peptide-based delivery vectors. Owing to the inherent poor enzymatic stability of peptides, CPPs displaying partial or full replacement of l-amino acids with the corresponding d-amino acids might possess advantages as delivery vectors. Thus, the present study aims to elucidate the membrane- and metabolism-associated effects of l-Penetratin (l-PEN) and its corresponding all-d analog (d-PEN). These effects were investigated when exerted on hepatocellular (HepG2) or intestinal (Caco-2 and IEC-6) cell culture models. The head-to-head comparison of these enantiomeric CPPs included evaluation of their effects on cell viability and morphology, epithelial membrane integrity, and cellular ultrastructure. In all investigated cell models, a rapid decrease in cell viability, pronounced membrane perturbation and an altered ultrastructure were detected upon exposure to d-PEN. At equimolar concentrations, these observations were less pronounced or even absent for cells exposed to l-PEN. Both CPPs remained stable for at least 2 h during exposure to proliferating cells (cultured for 24 h), although d-PEN exhibited a longer half-life when compared with that of l-PEN when exposed to well-differentiated cell monolayers (cultured for 18–20 days). Thus, the stereochemistry of the CPP penetratin significantly influences its effects on cell viability and epithelial integrity when profiled against a panel of mammalian cells.

Membrane potential drives direct translocation of cell-penetrating peptides

Nanoscale ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 1949-1958 ◽  
Author(s):  
Xinli Gao ◽  
Song Hong ◽  
Zhiping Liu ◽  
Tongtao Yue ◽  
Jure Dobnikar ◽  
...  
Keyword(s):  
Molecular Dynamics ◽  
Membrane Potential ◽  
Electrostatic Potential ◽  
Cell Penetrating Peptides ◽  
Cell Penetrating

We report the molecular dynamics evidence for the direct translocation of CPPs across the membrane driven by the membrane electrostatic potential.

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