scholarly journals Scattering versus Fluorescence Self-Quenching: More than a Question of Faith for the Quantification of Water Flux in Large Unilamellar Vesicles?

2021 ◽  
Author(s):  
Johann Wachlmayr ◽  
Christof Hannesschlaeger ◽  
Armin Speletz ◽  
Thomas Barta ◽  
Anna Eckerstorfer ◽  
...  
Keyword(s):  
Water Flow ◽  
Water Flux ◽  
Polymer Membrane ◽  
Membrane Channels ◽  
Unilamellar Vesicles ◽  
Water Permeation ◽  
Channel Function ◽  
Large Unilamellar Vesicles

The endeavors to understand the determinants of water permeation through membrane channels, the effect of the lipid or polymer membrane on channel function, the development of specific water flow inhibitors,...

scholarly journals Quantification of Water Flux in Large Unilamellar Vesicles

2020 ◽  
Vol 118 (3) ◽  
pp. 136a-137a
Author(s):  
Christof Hannesschläger ◽  
Anna Eckerstorfer ◽  
Armin Speletz ◽  
Thomas Barta ◽  
Johann Wachlmayr ◽  
...  
Keyword(s):  
Water Flux ◽  
Unilamellar Vesicles ◽  
Large Unilamellar Vesicles

Water permeability of apical and basolateral cell membranes of rat inner medullary collecting duct

1990 ◽  
Vol 259 (6) ◽  
pp. F986-F999 ◽  
Author(s):  
B. Flamion ◽  
K. R. Spring
Keyword(s):  
Water Flow ◽  
Water Permeability ◽  
Cell Membranes ◽  
Apical Membrane ◽  
Collecting Duct ◽  
Basolateral Membrane ◽  
Volume Regulatory Decrease ◽  
Water Permeation ◽  
Inner Medullary Collecting Duct ◽  
Medullary Collecting Duct

To quantify the pathways for water permeation through the kidney medulla, knowledge of the water permeability (Posmol) of individual cell membranes in inner medullary collecting duct (IMCD) is required. Therefore IMCD segments from the inner two thirds of inner medulla of Sprague-Dawley rats were perfused in vitro using a setup devised for rapid bath and luminal fluid exchanges (half time, t1/2, of 55 and 41 ms). Differential interference contrast microscopy, coupled to video recording, was used to measure volume and approximate surface areas of single cells. Volume and volume-to-surface area ratio of IMCD cells were strongly correlated with their position along the inner medullary axis. Transmembrane water flow (Jv) was measured in response to a variety of osmotic gradients (delta II) presented on either basolateral or luminal side of the cells. The linear relation between Jv and delta II yielded the cell membrane Posmol, which was then corrected for membrane infoldings. Basolateral membrane Posmol was 126 +/- 3 microns/s. Apical membrane Posmol rose from a basal value of 26 +/- 3 microns/s to 99 +/- 5 microns/s in presence of antidiuretic hormone (ADH). Because of amplification of basolateral membrane, the ADH-stimulated apical membrane remained rate-limiting for transcellular osmotic water flow, and the IMCD cell did not swell significantly. Calculated transcellular Posmol, expressed in terms of smooth luminal surface, was 64 microns/s without ADH and 207 microns/s with ADH. IMCD cells in anisosmotic media displayed almost complete volume regulatory decrease but only partial volume regulatory increase.

Influence of the Coagulation-Bath Temperature on Membrane Formation Mechanism and Properties of PVDF/TA-ATP Hybrid Membrane

2015 ◽  
Vol 713-715 ◽  
pp. 2723-2726
Author(s):  
Yue Rong Shi ◽  
Gui Fang Zhang ◽  
Xing Tian Liu ◽  
Xue Tao Tian ◽  
Yi Ping Zhao ◽  
...  
Keyword(s):  
Formation Mechanism ◽  
Vinylidene Fluoride ◽  
Pure Water ◽  
Water Flux ◽  
Bath Temperature ◽  
Time Domain Reflectometry ◽  
Coagulation Bath ◽  
Membrane Formation ◽  
Water Permeation ◽  
Ultrasonic Time

In this study, poly (vinylidene fluoride) (PVDF) hybrid membranes were prepared from polymeric blend of PVDF/tannic acid (TA)-Attapulgite (ATP)/PEG system via phase inversion induced by immersion precipitation in water coagulation bath. The membrane formation mechanism of PVDF/TA-ATP/PEG casting solutions in water bath thermodynamics at different temperatures and the process of membrane formation were investigated via cloud point determination and ultrasonic time-domain reflectometry (UTDR). The structures and properties of the membrane were characterized by scanning electron microscopy (SEM), and water permeation experiment, respectively. It was found that the rate of precipitation controlled by the Coagulation-Bath thermodynamics. With the coagulation temperature increasing, the gelation line was moved to non-solvent axis and the pure water flux were decreased.

scholarly journals α-Synuclein Binds Large Unilamellar Vesicles as an Extended Helix†

Biochemistry ◽  
2009 ◽  
Vol 48 (11) ◽  
pp. 2304-2306 ◽  
Author(s):  
Adam J. Trexler ◽  
Elizabeth Rhoades
Keyword(s):  
Unilamellar Vesicles ◽  
Large Unilamellar Vesicles

Evolution of the thermotropic properties of large unilamellar vesicles (LUV)

1984 ◽  
Vol 40 (7) ◽  
pp. 715-717 ◽  
Author(s):  
M. Vandenbranden ◽  
C. Stil ◽  
R. Brasseur ◽  
J. -M. Ruysschaert
Keyword(s):  
Unilamellar Vesicles ◽  
Large Unilamellar Vesicles ◽  
Thermotropic Properties

The Efficacies of Cell-Penetrating Peptides in Accumulating in Large Unilamellar Vesicles Depend on their Ability To Form Inverted Micelles

ChemBioChem ◽  
2014 ◽  
Vol 15 (6) ◽  
pp. 884-891 ◽  
Author(s):  
Jean-Marie Swiecicki ◽  
Annika Bartsch ◽  
Julien Tailhades ◽  
Margherita Di Pisa ◽  
Benjamin Heller ◽  
...  
Keyword(s):  
Cell Penetrating Peptides ◽  
Unilamellar Vesicles ◽  
Large Unilamellar Vesicles ◽  
Cell Penetrating

Influence of Hydrophilic Polymer on Pure Water Permeation, Permeability Coefficient, and Porosity of Polysulfone Blend Membranes

2014 ◽  
Vol 931-932 ◽  
pp. 168-172 ◽  
Author(s):  
Asmadi Ali ◽  
Mohamad Awang ◽  
Ramli Mat ◽  
Anwar Johari ◽  
Mohd Johari Kamaruddin ◽  
...  
Keyword(s):  
Permeability Coefficient ◽  
Pure Water ◽  
Polymer Concentration ◽  
Water Flux ◽  
Hydrophilic Polymer ◽  
Hydrophobic Property ◽  
Water Permeation ◽  
Blend Membranes ◽  
Wet Phase Inversion ◽  
Pure Water Flux

It is well known that membrane with hydrophobic property is a fouling membrane. Polysulfone (PSf) membrane has hydrophobic characteristic was blended with a hydrophilic polymer, cellulose acetate phthalate (CAP) in order to increase hydrophilicity property of pure PSf membrane. In this study, membrane casting solutions containing 17 wt% of polymer was prepared via wet phase inversion process. The pure PSf membrane was coded as PC-0. PSf/CAP blend membranes with blend composition of 95/5, 90/10, 85/15 and 80/20 wt% of total polymer concentration in the membrane casting solutions were marked as PC-5, PC-10, PC-15 and PC-20 respectively. All of the membranes were characterized in terms of pure water flux and permeability coefficient in order to study their hydrophilicity properties. The investigated results shows that increased of CAP composition in PSf blend membranes has increased pure water flux, permeability coefficient and porosity of the blend membrane which in turn formed membrane with anti-fouling property.

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