Cell-internalizing nucleic acid aptamers: challenges and cellular uptake mechanisms for applications as pharmacological agents

2021 ◽  
Author(s):  
Samira Husen Alamudi ◽  
Michiko Kimoto ◽  
Ichiro Hirao
Keyword(s):  
Nucleic Acid ◽  
Cellular Uptake ◽  
In Vitro Selection ◽  
Pharmacological Agents ◽  
Nucleic Acid Aptamers ◽  
Uptake Mechanisms ◽  
Targeted Therapeutic

Nucleic acid aptamers, also regarded as chemical antibodies, manifest potentials for targeted therapeutic and delivery agents since they possess unique advantages over antibodies. Generated by an iterative in vitro selection...

[20] In vitro selection of nucleic acid aptamers that bind proteins

1996 ◽  
pp. 336-367 ◽  
Author(s):  
Richard C. Conrad ◽  
Lori Giver ◽  
Yu Tian ◽  
Andrew D. Ellington
Keyword(s):  
Nucleic Acid ◽  
In Vitro Selection ◽  
Nucleic Acid Aptamers ◽  
Selection Of

scholarly journals Hybrid-Type SELEX for the Selection of Artificial Nucleic Acid Aptamers Exhibiting Cell Internalization Activity

Pharmaceutics ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 888
Author(s):  
Hiro Uemachi ◽  
Yuuya Kasahara ◽  
Keisuke Tanaka ◽  
Takumi Okuda ◽  
Yoshihiro Yoneda ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Surface Protein ◽  
Functional Screening ◽  
Nucleic Acid Aptamers ◽  
Hybrid Type ◽  
Cell Internalization ◽  
Promising Target ◽  
A Cell ◽  
Exponential Enrichment

Nucleic acid aptamers have attracted considerable attention as next-generation pharmaceutical agents and delivery vehicles for small molecule drugs and therapeutic oligonucleotides. Chemical modification is an effective approach for improving the functionality of aptamers. However, the process of selecting appropriately modified aptamers is laborious because of many possible modification patterns. Here, we describe a hybrid-type systematic evolution of ligands by exponential enrichment (SELEX) approach for the generation of the artificial nucleic acid aptamers effective against human TROP2, a cell surface protein identified by drug discovery as a promising target for cancer therapy. Capillary electrophoresis SELEX was used for the pre-screening of multiple modified nucleic acid libraries and enrichment of TROP2 binding aptamers in the first step, followed by functional screening using cell-SELEX in the second step for the generation of cell-internalizing aptamers. One representative aptamer, Tac-B1, had a nanomolar-level affinity to human TROP2 and exhibited elevated capacity for internalization by cells. Because of the growing interest in the application of aptamers for drug delivery, our hybrid selection approach has great potential for the generation of functional artificial nucleic acid aptamers with ideal modification patterns in vitro.

scholarly journals In vitro dosimetry of agglomerates

Nanoscale ◽  
2014 ◽  
Vol 6 (13) ◽  
pp. 7325-7331 ◽  
Author(s):  
V. Hirsch ◽  
C. Kinnear ◽  
L. Rodriguez-Lorenzo ◽  
C. A. Monnier ◽  
B. Rothen-Rutishauser ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Diffusion Coefficients ◽  
Uptake Kinetics ◽  
Small Particles ◽  
Au Nps ◽  
Kinetics Of ◽  
Lower Diffusion ◽  
Uptake Mechanisms ◽  
In Vitro Dosimetry

A well-controlled route towards biocompatible agglomerated Au-NPs is reported, which span the range from small particles with high diffusion coefficients to larger particles with lower diffusion coefficients. Difference in uptake kinetics of single NPs and agglomerates can be explained by particokinetics, without the need to consider size-mediated cellular uptake mechanisms.

In vitro selection of high-affinity nucleic acid ligands to parasite target molecules

2003 ◽  
Vol 33 (12) ◽  
pp. 1309-1317 ◽  
Author(s):  
H.Ulrich Göringer ◽  
Matthias Homann ◽  
Mihaela Lorger
Keyword(s):  
Nucleic Acid ◽  
In Vitro Selection ◽  
High Affinity ◽  
Target Molecules ◽  
Selection Of
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