Characterization of interconnectivity of gelatin methacrylate hydrogels using photoacoustic imaging

Lab on a Chip ◽  
2022 ◽  
Author(s):  
Wenxiu Zhao ◽  
Haibo Yu ◽  
Zhixing Ge ◽  
Xiaoduo Wang ◽  
Yuzhao Zhang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Extracellular Matrix ◽  
Photoacoustic Imaging ◽  
Three Dimensional

Hydrogels can provide a three-dimensional microenvironment for cells and thus serve as an extracellular matrix in a biofabrication process. The properties of hydrogels, such as their porosity and mechanical properties,...

A bioartificial rat heart tissue: Perfusion decellularization and characterization

2019 ◽  
Vol 42 (12) ◽  
pp. 757-764 ◽  
Author(s):  
Busra Ozlu ◽  
Mert Ergin ◽  
Sevcan Budak ◽  
Selcuk Tunali ◽  
Nuh Yildirim ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Three Dimensional ◽  
Tissue Perfusion ◽  
Heart Tissue ◽  
Cell Nuclei ◽  
Tissue Sections ◽  
The Past ◽  
Significant Difference ◽  
Structural Preservation

Despite remarkable advancement in the past decades, heart-related defects are still prone to progress irreversibly and can eventually lead to heart failure. A personalized extracellular matrix–based bioartificial heart created by allografts/xenografts emerges as an alternative as it can retain the original three-dimensional architecture combined with a preserved natural heart extracellular matrix. This study aimed at developing a procedure for decellularizing heart tissue harvested from rats and evaluating decellularization efficiency in terms of residual nuclear content and structural properties. Tissue sections showed no or little visible cell nuclei in decellularized heart, whereas the native heart showed dense cellularity. In addition, there was no significant variation in the alignment of muscle fibers upon decellularization. Furthermore, no significant difference was detected between native and decellularized hearts in terms of fiber diameter. Our findings demonstrate that fiber alignment and diameter can serve as additional parameters in the characterization of biological heart scaffolds as these provide valuable input for evaluating structural preservation of decellularized heart. The bioartificial scaffold formed here can be functionalized with patient’s own material and utilized in regenerative engineering.

Mechanical Characterization of the Decellularized Porcine Small Intestinal Submucosa Extracellular Matrix

2013 ◽  
Author(s):  
Shijia Zhao ◽  
Linxia Gu ◽  
James M. Hammel ◽  
Haili Lang
Keyword(s):  
Mechanical Properties ◽  
Extracellular Matrix ◽  
Mechanical Response ◽  
Mechanical Characterization ◽  
Small Intestinal Submucosa ◽  
Trade Name ◽  
Porcine Small Intestinal Submucosa ◽  
Small Intestinal ◽  
Intestinal Submucosa

In this work, the decellularized porcine small intestinal submucosa extracellular matrix (SIS-ECM), obtained from the commercial product under the trade name of CorMatrix, were tested in uniaxial tension. Preconditioning under cyclic loading of 2 N was conducted to stabilize the mechanical response of the tissue. The influence of rehydration time on the mechanical properties of the tissue was evaluated. Results suggested that the stiffness of SIS-ECM decreased with longer rehydration time. Considering the application of CorMatrix in pericardial closure, the native pericardium samples were also tested. The comparison indicated that the native pericardium is softer than rehydrated CorMatrix. This work can facilitate the surgeons to better choose the appropriate rehydration time when conducting the extracardiac implantations, such as pericardial reconstruction, pericardial closure, etc.

Characterization of the Naked Mole Rat Incisors: Chemical Composition, Microstructure and Mechanical Properties

2021 ◽  
pp. 1-10
Author(s):  
Hongyan Qi ◽  
Guixiong Gao ◽  
Huixin Wang ◽  
Yunhai Ma ◽  
Hubiao Wang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Chemical Composition ◽  
Lamellar Structure ◽  
Three Dimensional ◽  
Microstructure And Mechanical Properties ◽  
Inorganic Matter ◽  
Mole Rat ◽  
Naked Mole Rat ◽  
Reticular Structure

The naked mole rat incisors (NMRI) exhibit excellent mechanical properties, which makes it a good prototype for design and fabrication of bionic mechanical systems and materials. In this work, we characterized the chemical composition, microstructure and mechanical properties of NMRI, and further compared these properties with the laboratory rat incisors (LRI). We found that (1) Enamel and dentin are composed of organic matter, inorganic matter and water. The ratio of Ca/P in NMRI enamel is higher than that of LRI enamel. (2) The dentin has a porous structure. The enamel has a three-dimensional reticular structure, which is more complex, regular and denser than the lamellar structure of LRI enamel. (3) Enamel has anisotropy. Its longitudinal nano-hardness is greater than that of transverse nano-hardness, and both of them are higher than that of LRI enamel. Their nano-hardness and elastic modulus increase with the increment of distance from the enamel-dentin boundary. The nano-hardness of dentin is smaller than that of enamel. The chemical composition and microstructure are considered to be the reasons for the excellent properties of NMRI. The chemical composition and unique microstructure can provide inspiration and guidelines for the design of bionic machinery and materials.

Fabrication and Characterization of Porous TiB2 Ceramic with Three-Dimensional Interconnected Skeleton

2007 ◽  
Vol 336-338 ◽  
pp. 1076-1079
Author(s):  
Chang Qing Hong ◽  
Jie Cai Han ◽  
Xing Hong Zhang ◽  
He Xin Zhang
Keyword(s):  
Mechanical Properties ◽  
High Temperature ◽  
Bending Strength ◽  
Three Dimensional ◽  
Pressureless Sintering ◽  
Neck Growth ◽  
Selective Heating ◽  
Fabrication And Characterization ◽  
Tib2 Particles

Porous TiB2 ceramics with a three-dimensional interconnected skeleton were fabricated by high temperature pressureless sintering from fine TiB2 powders. The microstructure of the porous TiB2 ceramic was characterized by the enhanced neck growth between the initially touching particles. This neck growth was ascribed to the selective heating of TiB2 particles with different dimension. The porous structure prepared by the high-temperature sintering exhibited higher bending strength and fracture toughness in the present experiment. The improved mechanical properties of the sintered composites were attributable to the enhanced neck growth by surface diffusion.

Fabrication and Characterization of P34HB Scaffolds with Oriented Microtubules

2014 ◽  
Vol 898 ◽  
pp. 318-321 ◽  
Author(s):  
Xin Hui Wang ◽  
Lin Sang ◽  
Zhi Yong Wei ◽  
Li Jie Zhai ◽  
Min Qi
Keyword(s):  
Mechanical Property ◽  
Extracellular Matrix ◽  
Pore Size ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Three Dimensional Structure ◽  
Significant Potential ◽  
Fabrication And Characterization

Sponge-like scaffold with a specific three-dimensional structure resembling the actual extracellular matrix of a particular tissue show significant potential for the regeneration and repair of damaged anisotropic tissues. In this research, an oriented microtubular P34HB scaffold was prepared successfully. The mechanical property showed that anisotropy of modulus is much greater than a typical non-oriented scaffold. Altering the P34HB concentration allowed P34HB scaffolds to be produced with complex pore orientations, and anisotropy in pore size and alignment.

scholarly journals 3D mechanical characterization of single cells and small organisms using acoustic manipulation and force microscopy

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Nino F. Läubli ◽  
Jan T. Burri ◽  
Julian Marquard ◽  
Hannes Vogler ◽  
Gabriella Mosca ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Single Cells ◽  
Three Dimensional ◽  
Pollen Grains ◽  
Lilium Longiflorum ◽  
Force Sensor ◽  
Cellular Growth ◽  
Apparent Stiffness ◽  
Micromechanical Characterization

AbstractQuantitative micromechanical characterization of single cells and multicellular tissues or organisms is of fundamental importance to the study of cellular growth, morphogenesis, and cell-cell interactions. However, due to limited manipulation capabilities at the microscale, systems used for mechanical characterizations struggle to provide complete three-dimensional coverage of individual specimens. Here, we combine an acoustically driven manipulation device with a micro-force sensor to freely rotate biological samples and quantify mechanical properties at multiple regions of interest within a specimen. The versatility of this tool is demonstrated through the analysis of single Lilium longiflorum pollen grains, in combination with numerical simulations, and individual Caenorhabditis elegans nematodes. It reveals local variations in apparent stiffness for single specimens, providing previously inaccessible information and datasets on mechanical properties that serve as the basis for biophysical modelling and allow deeper insights into the biomechanics of these living systems.

scholarly journals Characterization of the content and distribution of collagen and proteoglycan in the decellularized book-shaped enthesis scaffolds by SR-FTIR

2021 ◽  
Author(s):  
Qiang Shi ◽  
Can Chen ◽  
Muzhi Li ◽  
Yang Chen ◽  
Yan Xu ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Rotator Cuff ◽  
Load Transfer ◽  
Mechanical Test ◽  
Three Dimensional ◽  
Decellularized Extracellular Matrix ◽  
Quantitative Mapping ◽  
Extracellular Matrix Molecules ◽  
Histological Morphology

Abstract Background: Bone-tendon interface (enthesis) plays a pivotal role in relaxing load transfer between otherwise structurally and functionally distinct tissue types. Currently, decellularized extracellular matrix (DEM) from enthesis provide a natural three-dimensional scaffold with tissue-specific orientations of extracellular matrix molecules for enthesis regeneration, however, the content and distribution of collagen and proteoglycan in the decellularized book-shaped enthesis scaffolds from rabbit rotator cuff by SR-FTIR have not been reported.Methods: Native enthesis tissues (NET) harvested from rabbit rotator cuff were sectioned into cuboid (about 30 mm × 1.2 mm × 10 mm) for decalcified. The decellularized book-shaped enthesis scaffolds were conducted and intrinsic ultrastructure was evaluated by histological staining and scanning electron microscopy (SEM), respectively. The content and distribution of collagen and proteoglycan in the decellularized book-shaped enthesis scaffolds from rabbit rotator cuff were also measured innovatively by SR-FTIR.Results: The decellularized book-shaped enthesis scaffolds from rabbit rotator cuff were successfully obtaine©d. Histomorphology and SEM evaluated the decellularized effect and the structure of extracellular matrix during decellularization. After mechanical test, we found the failure load in the NET group was higher than that in the DEM group (P < 0.05), reached 1.32 times as much as that in the DEM group. Meanwhile, the stiffness of the DEM group was significantly lower than the NET group. Furthermore, the distributions of collagen and PGs content in the decellularized book-shaped enthesis scaffolds were decreased obviously after decellularization by SR-FTIR quantitative analysis.Conclusion: SR-FTIR was applied innovatively to characterize the histological morphology of native enthesis tissues from rabbit rotator cuff. Moreover, it can be used for quantitative mapping of the content and distribution of collagen and PGs content in the decellularized book-shaped enthesis scaffolds.

Structural characterization of heparinase III from Bacteroides thetaiotaomicron

2014 ◽  
Vol 70 (a1) ◽  
pp. C480-C480
Author(s):  
Thirumalaiselvi Ulaganathan ◽  
Maia Cherney ◽  
Deqiang Yao ◽  
Guoyun Li ◽  
Lingyun Li ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Three Dimensional ◽  
Nutrient Content ◽  
Site Directed Mutagenesis ◽  
Bacteroides Thetaiotaomicron ◽  
Connective Tissues ◽  
Crystal Forms ◽  
Repeat Units ◽  
Polysaccharide Lyases

Glycosaminoglycans (GAGs) are linear polysaccharides comprised of disaccharide repeat units, a hexuronic acid, glucuronic acid (GlcA) or iduronic acid (IdoA), linked to a hexosamine, N-acetylglucosamine (GlcNAc) or N-acetylgalactosamine (GalNAc). GAGs undergo further modification such as epimerization and sulfation. These polysaccharides are abundant in the extracellular matrix and connective tissues. GAGs function in stabilization of the fibrillar extracellular matrix (ECM), control of hydration, regulation of tissue, organism development by controlling cell cycle, cell behavior, and differentiation. Niche adapted bacteria expresses enzymes called polysaccharide lyases (PL), which degrade GAGs for their nutrient content. Polysaccharide lyases have been classified into 23 sequence-related families. Comparison of three-dimensional structures of the prototypic members of these families allowed identification of distant evolutionary relationships between lyases that were unrecognized at the sequence level and identified occurrences of convergent evolution. We have characterized structurally and enzymatically Heparinase III (HepIII) from Bacteroides thetaiotaomicron, which is classified within the PL12 family. HepIII is a 72.5KDa protein. We will present the X-Ray structures of two crystal forms of HepIII of resolution 1.8 Å and 2.6 Å. HepIII contains two domains, the N-terminal α-helical domain forming a toroid and the C-terminal β-sheet domain. Comparison with recently determined structures of two other heparinases from the same PL12 family allowed us to identify structural flexibility in the arrangement of the domains indicating open-close movement. Based on comparison with other GAG lyases we identified Tyr301 as the main catalytic residue and confirmed this by site-directed mutagenesis. We have characterized substrate preference of HepIII toward sulfate poor heparan sulcate substrate.

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