A Continuous Flow PCR Array Microfluidic Chip Applied for Simultaneous Amplification of Target Genes of Periodontal Pathogens

Lab on a Chip ◽  
2022 ◽  
Author(s):  
Bo Yang ◽  
Ping Wang ◽  
Zhenqing Li ◽  
Chunxian Tao ◽  
Qingxiang You ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Microfluidic Chip ◽  
Continuous Flow ◽  
Target Genes ◽  
Pcr Array ◽  
Periodontal Pathogens ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Time Required

The concept of time to place conversion makes continuous flow polymerase chain reaction (CF-PCR) microfluidic chip an ideal way to reduce the time required for amplification of target genes, however,...

An extrusion fluidic driving method for continuous-flow polymerase chain reaction on a microfluidic chip

2009 ◽  
Vol 168 (1-2) ◽  
pp. 71-78 ◽  
Author(s):  
Zhang-Run Xu ◽  
Xin Wang ◽  
Xiao-Feng Fan ◽  
Jian-Hua Wang
Keyword(s):  
Polymerase Chain Reaction ◽  
Microfluidic Chip ◽  
Continuous Flow ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Clinical evaluation of periodontal pathogen levels by real-time polymerase chain reaction in peri-implantitis patients

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Taichi Ito ◽  
Gentaro Mori ◽  
Yukari Oda ◽  
Tomoki Hirano ◽  
Hodaka Sasaki ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Bacterial Flora ◽  
Periodontal Pathogen ◽  
Periodontal Pathogens ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Average Copy ◽  
Number Of Bacteria ◽  
Total Bacteria

Abstract Objective The mechanisms underlying the onset and progression of peri-implantitis are similar to those of periodontitis, and the causative bacteria are believed to similar. Previous studies support an association between peri-implantitis and periodontal pathogen. Thus, we investigated the bacterial flora of peri-implantitis patients in comparison to those of healthy implant and periodontitis patients. Materials and methods In total, 70 patients visiting Tokyo Dental College Chiba Hospital were divided into four groups: healthy, periodontitis, healthy implant, and peri-implantitis. For each group, the following five periodontal pathogens were detected using real-time polymerase chain reaction: Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Treponema denticola, and Prevotella intermedia. Results The average copy number of total bacteria was significantly higher in the periodontitis group than in the other groups. P. gingivalis was detected in the periodontitis and peri-implantitis groups at levels as high as 18.92% and 12.29%, respectively, and P. intermedia was found in the peri-implantitis group at a rate of 2.06%. Nevertheless, periodontal pathogens were generally detected at lower levels in the peri-implantitis group than in the periodontitis group. Conclusion We found lower bacterial counts in the peri-implantitis group relative to the periodontitis group. Our results suggest that the peri-implant tissue is less resistant to bacteria, so even a small number of bacteria can be a risk factor for peri-implantitis and the causative agent of peri-implantitis can be bacteria other than periodontal pathogen.

scholarly journals Continuous flow polymerase chain reaction using a hybrid PMMA-PC microchip with improved heat tolerance

2008 ◽  
Vol 130 (2) ◽  
pp. 836-841 ◽  
Author(s):  
Yi Sun ◽  
M.V.D. Satyanarayan ◽  
Nam Trung Nguyen ◽  
Yien Chian Kwok
Keyword(s):  
Polymerase Chain Reaction ◽  
Heat Tolerance ◽  
Continuous Flow ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Evaluation of Amplification Targets for the Specific Detection ofBordetella pertussisUsing Real-Time Polymerase Chain Reaction

2014 ◽  
Vol 25 (4) ◽  
pp. 217-221 ◽  
Author(s):  
Mohammad Rubayet Hasan ◽  
Rusung Tan ◽  
Ghada N Al-Rawahi ◽  
Eva Thomas ◽  
Peter Tilley
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Target Genes ◽  
Reference Panel ◽  
Superior Performance ◽  
Clinical Samples ◽  
Analytical Sensitivity ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Polymerase Chain

BACKGROUND:Bordetella pertussisinfections continue to be a major public health challenge in Canada. Polymerase chain reaction (PCR) assays to detectB pertussisare typically based on the multicopy insertion sequence IS481, which offers high sensitivity but lacks species specificity.METHODS: A novelB pertussisreal-time PCR assay based on the porin gene was tested in parallel with several previously published assays that target genes such as IS481,ptx-promoter, pertactin and a putative thialase. The assays were evaluated using a reference panel of common respiratory bacteria including differentBordetellaspecies and 107 clinical nasopharyngeal specimens. Discrepant results were confirmed by sequencing the PCR products.RESULTS: Analytical sensitivity was highest for the assay targeting the IS481element; however, the assay lacked specificity forB pertussisin the reference panel and in the clinical samples. False-positive results were also observed with assays targeting theptx-promoter and pertactin genes. A PCR assay based on the thialase gene was highly specific but failed to detect all reference strains ofB pertussis. However, a novel assay targeting the porin gene demonstrated high specificity forB pertussisboth in the reference panel and in clinical samples and, based on sequence-confirmed results, correctly predicted allB pertussis-positive cases in clinical samples. According to Probit regression analysis, the 95% detection limit of the new assay was 4 colony forming units/reaction.CONCLUSION: A novel porin assay forB pertussisdemonstrated superior performance and may be useful for improved molecular detection ofB pertussisin clinical specimens.

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