A microfluidic platform enables comprehensive gene expression profiling of mouse retinal stem cells

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Shana O Kelley ◽  
Mahmoud Labib ◽  
Brenda Coles ◽  
Mahla Poudineh ◽  
Brendan Innes ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Stem Cell ◽  
Visual Impairment ◽  
Gene Expression Profiling ◽  
Replacement Therapy ◽  
Retinal Degeneration ◽  
Expression Profiling ◽  
Cell Replacement Therapy ◽  
Cell Replacement

Loss of photoreceptors due to retinal degeneration is a major cause of untreatable visual impairment and blindness. Cell replacement therapy, using retinal stem cell (RSC)-derived photoreceptors, holds promise for reconstituting...

scholarly journals Different Isolation Methods Alter the Gene Expression Profiling of Adipose Derived Stem Cells

2014 ◽  
Vol 11 (4) ◽  
pp. 391-403 ◽  
Author(s):  
Nareshwaran Gnanasegaran ◽  
Vijayendran Govindasamy ◽  
Sabri Musa ◽  
Noor Hayaty Abu Kasim
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Adipose Derived Stem Cells ◽  
Isolation Methods

Gene Expression Profiling of Neural Stem Cells and Identification of Regulators of Neural Differentiation During Cortical Development

Stem Cells ◽  
2011 ◽  
Vol 29 (11) ◽  
pp. 1817-1828 ◽  
Author(s):  
Toshiyuki Ohtsuka ◽  
Hiromi Shimojo ◽  
Mitsuhiro Matsunaga ◽  
Naoki Watanabe ◽  
Kohei Kometani ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Neural Stem Cells ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Neural Differentiation ◽  
Cortical Development

scholarly journals ENTPD3 Marks Mature Stem Cell Derived Beta Cells Formed by Self-Aggregation in Vitro

2021 ◽  
Author(s):  
Fiona M. Docherty ◽  
Kent A. Riemondy ◽  
Roberto Castro-Gutierrez ◽  
JaeAnn M. Dwulet ◽  
Ali H. Shilleh ◽  
...  
Keyword(s):  
Stem Cell ◽  
Replacement Therapy ◽  
Beta Cells ◽  
Nucleoside Triphosphate ◽  
Specific Marker ◽  
Detailed Knowledge ◽  
Cell Replacement Therapy ◽  
Mature Adult ◽  
Cell Replacement

Stem cell derived beta-like cells (sBC) carry the promise of providing an abundant source of insulin-producing cells for use in cell replacement therapy for patients with diabetes, potentially allowing widespread implementation of a practical cure. To achieve their clinical promise, sBC need to function comparably to mature adult beta cells, but as yet they display varying degrees of maturity. Indeed, detailed knowledge of the events resulting in human beta cell maturation remains obscure. Here we show that sBC spontaneously self-enrich into discreet islet-like cap structures within <i>in vitro</i> cultures, independent of exogenous maturation conditions. Multiple complementary assays demonstrate that this process is accompanied by functional maturation of the self-enriched sBC (seBC); however, the seBC still contain distinct subpopulations displaying different maturation levels. Interestingly, the surface protein ENTPD3 (also known as nucleoside triphosphate diphosphohydrolase-3 (NDPTase3)) is a specific marker of the most mature seBC population and can be used for mature seBC identification and sorting. Our results illuminate critical aspects of <i>in vitro</i> sBC maturation and provide important insights towards developing functionally mature sBC for diabetes cell replacement therapy.

scholarly journals Ca(2+) Oscillations and Its Transporters in Mesenchymal Stem Cells

2010 ◽  
pp. 323-329 ◽  
Author(s):  
B Ye
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Replacement Therapy ◽  
Cell Types ◽  
Cell Replacement Therapy ◽  
Cell Replacement ◽  
Cell Functions ◽  
The Past ◽  
Intracellular Ca

Intracellular free Ca(2+) is one of important biological signals regulating a number of cell functions. It has been discussed widely and extensively in several cell types during the past two decades. Attention has been paid to the Ca2+ transportation in mesenchymal stem cells in recent years as mesenchymal stem cells have gained considerable interest due to their potential for cell replacement therapy and tissue engineering. In this paper, roles of intracellular Ca(2+) oscillations and its transporters in mesenchymal stem cells have been reviewed.

Gene expression profiling of bone marrow mesenchymal stem cells from Osteogenesis Imperfecta patients during osteoblast differentiation

2017 ◽  
Vol 60 (6) ◽  
pp. 326-334 ◽  
Author(s):  
Carla Martins Kaneto ◽  
Patrícia S. Pereira Lima ◽  
Karen Lima Prata ◽  
Jane Lima dos Santos ◽  
João Monteiro de Pina Neto ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Osteogenesis Imperfecta ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Osteoblast Differentiation ◽  
Marrow Mesenchymal Stem Cells

Gene Expression Profiling of Stem Cells by Microarray

2007 ◽  
pp. 149-161 ◽  
Author(s):  
Timothy McDaniel ◽  
Shawn Baker ◽  
David Barker ◽  
Roy Williams ◽  
Franz-Josef Mueller
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Gene Expression Profiling ◽  
Expression Profiling

scholarly journals The hematopoietic stem cells of alpha-thalassemic mice

Blood ◽  
1985 ◽  
Vol 66 (3) ◽  
pp. 595-601 ◽  
Author(s):  
JE Barker ◽  
E McFarland
Keyword(s):  
Stem Cells ◽  
Replacement Therapy ◽  
Host Cells ◽  
Stem Cell Population ◽  
Normal Donor ◽  
Potential Candidate ◽  
Cell Replacement Therapy ◽  
Hematopoietic Stem ◽  
Cell Replacement ◽  
Marrow Cells

Abstract The alpha-thalassemic mouse has a hereditary microcytic anemia, almost certainly has a shortened RBC life span, and is a potential candidate for cell replacement therapy. In a routine study of bone marrow repopulating capacity using hemoglobin as a cell marker, normal donor marrow cells, but not alpha-thalassemic donor marrow cells, completely replaced the host cells. Further analysis showed that at least 30 times more alpha-thalassemic cells were required to outcompete normal donor cells injected simultaneously. The results were more extreme then expected and suggested a defect in a stem cell population as well as in the RBCs. Evidence that the multipotent and erythroid-committed stem cells in alpha-thalassemic mice are not decreased was shown by CFU-S and CFU-E assays. The combined results indicate that the deletion expresses itself most conspicuously in the RBC population. Tests were also performed to analyze repopulation kinetics in the Hbath-J/+ mice. In unirradiated alpha-thalassemic hosts, the hemoglobin from a normal donor persisted but did not replace the host hemoglobin. Sublethally irradiated alpha-thalassemic hosts, on the other hand, were easily repopulated with normal cells. We conclude that the alpha-thalassemic mouse is a good model for cell replacement therapy.

Sign in / Sign up

Export Citation Format

Share Document