A photofabricated honeycomb micropillar array for loss-free trapping of microfluidic droplets and application to digital PCR

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Yu He ◽  
Zefan Lu ◽  
Hongliang Fan ◽  
Tao Zhang
Keyword(s):  
Biomedical Applications ◽  
Digital Pcr ◽  
Droplet Microfluidics ◽  
Microfluidic Droplets ◽  
Micropillar Array

Droplet microfluidics is a promising platform for various biological and biomedical applications. Among which, droplet-based digital PCR (ddPCR) is one of the most challenging examples, with practical issues involving possible...

Droplet-based microfluidics in biomedical applications

2021 ◽  
Author(s):  
Leyla Amirifar ◽  
Mohsen Besanjideh ◽  
Rohollah Nasiri ◽  
Amir Shamloo ◽  
Fatemeh Nasrollahi ◽  
...  
Keyword(s):  
Drug Delivery ◽  
High Throughput ◽  
Paradigm Shift ◽  
Cell Biology ◽  
Biomedical Applications ◽  
Molecular Detection ◽  
Droplet Microfluidics ◽  
Droplet Generation ◽  
Microfluidic Platforms ◽  
Standard Methods

Abstract Droplet-based microfluidic systems have been employed to manipulate discrete fluid volumes with immiscible phases. Creating the fluid droplets at microscale has led to a paradigm shift in mixing, sorting, encapsulation, sensing, and designing high throughput devices for biomedical applications. Droplet microfluidics has opened many opportunities in microparticle synthesis, molecular detection, diagnostics, drug delivery, and cell biology. In the present review, we first introduce standard methods for droplet generation (i.e., passive and active methods) and discuss the latest examples of emulsification and particle synthesis approaches enabled by microfluidic platforms. Then, the applications of droplet-based microfluidics in different biomedical applications are detailed. Finally, a general overview of the latest trends along with the perspectives and future potentials in the field are provided.

scholarly journals Precise pooling and dispensing of microfluidic droplets towards micro- to macro-world interfacing

RSC Advances ◽  
2014 ◽  
Vol 4 (73) ◽  
pp. 38542-38550 ◽  
Author(s):  
Eric Brouzes ◽  
April Carniol ◽  
Tomasz Bakowski ◽  
Helmut H. Strey
Keyword(s):  
Microfluidic Device ◽  
Droplet Microfluidics ◽  
Seamless Integration ◽  
Microfluidic Droplets ◽  
Microtiter Plates

We present the design of a microfluidic device that accurately and automatically pools and delivers a precise number of droplets. It will permit the interfacing of the microfluidic world to the macrofluidic world (e.g. microtiter plates) and will allow the seamless integration of droplet microfluidics into already developed robotic workflows.

scholarly journals Dual sequentially addressable dielectrophoretic array for high-throughput, scalable, multiplexed droplet sorting

2021 ◽  
Vol 25 (4) ◽  
Author(s):  
Akihiro Isozaki ◽  
Dunhou Huang ◽  
Yuta Nakagawa ◽  
Keisuke Goda
Keyword(s):  
High Throughput ◽  
Cell Biology ◽  
Digital Pcr ◽  
Industrial Applications ◽  
Droplet Microfluidics ◽  
Diverse Range ◽  
Droplet Sorting ◽  
On Chip ◽  
Single Droplets

AbstractDroplet microfluidics is a powerful tool for a diverse range of biomedical and industrial applications such as single-cell biology, synthetic biology, digital PCR, biosafety monitoring, drug screening, and food, feed, and cosmetic industries. As an integral part of droplet microfluidics, on-chip multiplexed droplet sorting has recently gained enthusiasm, since it enables real-time sorting of single droplets containing cells with different phenotypes into multiple bins. However, conventional sorting methods are limited in throughput and scalability. Here, we present high-throughput, scalable, multiplexed droplet sorting by employing a pair of sequentially addressable dielectrophoretic arrays (SADAs) across a microchannel on a microfluidic chip. A SADA is an on-chip array of electrodes, each of which is sequentially activated and deactivated in synchronization to the position and speed of a flowing droplet of interest. The dual-SADA (dSADA) structure enables high-throughput deflection of droplets in multiple directions in a well-controlled manner. For proof-of-concept demonstration and characterization of the dSADA, we performed fluorescence-activated droplet sorting (FADS) with a 3-way dSADA at a high throughput of 2450 droplets/s. Furthermore, to show the scalability of the dSADA, we also performed FADS with a 5-way dSADA at a high throughput of 473 droplets/s.

Quantum-dot-encapsulated core–shell barcode particles from droplet microfluidics

2018 ◽  
Vol 6 (44) ◽  
pp. 7257-7262 ◽  
Author(s):  
Feika Bian ◽  
Huan Wang ◽  
Lingyu Sun ◽  
Yuxiao Liu ◽  
Yuanjin Zhao
Keyword(s):  
Quantum Dot ◽  
Microfluidic Device ◽  
Biomedical Applications ◽  
Droplet Microfluidics ◽  
Core Shell ◽  
New Class

A new class of QD-encapsulated core–shell barcode particles for biomedical applications were generated using a capillary microfluidic device.

scholarly journals Microfluidic fabrication of microparticles for biomedical applications

2018 ◽  
Vol 47 (15) ◽  
pp. 5646-5683 ◽  
Author(s):  
Wen Li ◽  
Liyuan Zhang ◽  
Xuehui Ge ◽  
Biyi Xu ◽  
Weixia Zhang ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Droplet Microfluidics ◽  
Biomedical Fields

This review summarizes microparticles produced by droplet microfluidics and their applications in biomedical fields.

Applications of Scanning Microscopy in Biomedical Research

1968 ◽  
Vol 26 ◽  
pp. 354-355
Author(s):  
T. L. Hayes
Keyword(s):  
Information Content ◽  
Biomedical Research ◽  
Biomedical Applications ◽  
Three Dimensional ◽  
Dental Enamel ◽  
Resolving Power ◽  
Whole Mount ◽  
Two Parameters ◽  
Content Information ◽  
Sectioned Tissue

Biomedical applications of the scanning electron microscope (SEM) have increased in number quite rapidly over the last several years. Studies have been made of cells, whole mount tissue, sectioned tissue, particles, human chromosomes, microorganisms, dental enamel and skeletal material. Many of the advantages of using this instrument for such investigations come from its ability to produce images that are high in information content. Information about the chemical make-up of the specimen, its electrical properties and its three dimensional architecture all may be represented in such images. Since the biological system is distinctive in its chemistry and often spatially scaled to the resolving power of the SEM, these images are particularly useful in biomedical research.In any form of microscopy there are two parameters that together determine the usefulness of the image. One parameter is the size of the volume being studied or resolving power of the instrument and the other is the amount of information about this volume that is displayed in the image. Both parameters are important in describing the performance of a microscope. The light microscope image, for example, is rich in information content (chemical, spatial, living specimen, etc.) but is very limited in resolving power.

How SIMS microscopy can be used in medicine

1992 ◽  
Vol 50 (2) ◽  
pp. 1602-1603
Author(s):  
Philippe Fragu
Keyword(s):  
Mass Spectrometry ◽  
Biomedical Applications ◽  
Secondary Ion Mass Spectrometry ◽  
Chemical Elements ◽  
Biological Applications ◽  
The Past ◽  
Potential Source ◽  
Secondary Ion ◽  
Chemical Integrity ◽  
Scientific Endeavour

The identification, localization and quantification of intracellular chemical elements is an area of scientific endeavour which has not ceased to develop over the past 30 years. Secondary Ion Mass Spectrometry (SIMS) microscopy is widely used for elemental localization problems in geochemistry, metallurgy and electronics. Although the first commercial instruments were available in 1968, biological applications have been gradual as investigators have systematically examined the potential source of artefacts inherent in the method and sought to develop strategies for the analysis of soft biological material with a lateral resolution equivalent to that of the light microscope. In 1992, the prospects offered by this technique are even more encouraging as prototypes of new ion probes appear capable of achieving the ultimate goal, namely the quantitative analysis of micron and submicron regions. The purpose of this review is to underline the requirements for biomedical applications of SIMS microscopy.Sample preparation methodology should preserve both the structural and the chemical integrity of the tissue.

Biomedical applications: Pitfalls in the practice

1994 ◽  
Vol 52 ◽  
pp. 378-379
Author(s):  
J. D. Shelburne ◽  
Peter Ingram ◽  
Victor L. Roggli ◽  
Ann LeFurgey
Keyword(s):  
Operating Room ◽  
Liquid Nitrogen ◽  
Lung Tissue ◽  
Biomedical Applications ◽  
Microprobe Analysis ◽  
Tissue Sample ◽  
Cellular Level ◽  
Tissue Samples ◽  
Asbestos Fibers

At present most medical microprobe analysis is conducted on insoluble particulates such as asbestos fibers in lung tissue. Cryotechniques are not necessary for this type of specimen. Insoluble particulates can be processed conventionally. Nevertheless, it is important to emphasize that conventional processing is unacceptable for specimens in which electrolyte distributions in tissues are sought. It is necessary to flash-freeze in order to preserve the integrity of electrolyte distributions at the subcellular and cellular level. Ideally, biopsies should be flash-frozen in the operating room rather than being frozen several minutes later in a histology laboratory. Electrolytes will move during such a long delay. While flammable cryogens such as propane obviously cannot be used in an operating room, liquid nitrogen-cooled slam-freezing devices or guns may be permitted, and are the best way to achieve an artifact-free, accurate tissue sample which truly reflects the in vivo state. Unfortunately, the importance of cryofixation is often not understood. Investigators bring tissue samples fixed in glutaraldehyde to a microprobe laboratory with a request for microprobe analysis for electrolytes.

Biological and biomedical applications of collagenous biomaterials

1990 ◽  
Vol 48 (3) ◽  
pp. 856-857
Author(s):  
Yasushi P. Kato ◽  
Michael G. Dunn ◽  
Frederick H. Silver ◽  
Arthur J. Wasserman
Keyword(s):  
Biomedical Applications ◽  
Soft Tissues ◽  
Collagen Fibers ◽  
Bone Collagen ◽  
Cover Slip ◽  
Fibroblast Migration ◽  
Cellular Expression ◽  
Defect Repair

Collagenous biomaterials have been used for growing cells in vitro as well as for augmentation and replacement of hard and soft tissues. The substratum used for culturing cells is implicated in the modulation of phenotypic cellular expression, cellular orientation and adhesion. Collagen may have a strong influence on these cellular parameters when used as a substrate in vitro. Clinically, collagen has many applications to wound healing including, skin and bone substitution, tendon, ligament, and nerve replacement. In this report we demonstrate two uses of collagen. First as a fiber to support fibroblast growth in vitro, and second as a demineralized bone/collagen sponge for radial bone defect repair in vivo.For the in vitro study, collagen fibers were prepared as described previously. Primary rat tendon fibroblasts (1° RTF) were isolated and cultured for 5 days on 1 X 15 mm sterile cover slips. Six to seven collagen fibers, were glued parallel to each other onto a circular cover slip (D=18mm) and the 1 X 15mm cover slip populated with 1° RTF was placed at the center perpendicular to the collagen fibers. Fibroblast migration from the 1 x 15mm cover slip onto and along the collagen fibers was measured daily using a phase contrast microscope (Olympus CK-2) with a calibrated eyepiece. Migratory rates for fibroblasts were determined from 36 fibers over 4 days.

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