Antibiotic susceptibility test under a linear concentration gradient using travelling surface acoustic waves

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Yongtaek Im ◽  
Seunggyu Kim ◽  
Jinsoo Park ◽  
Hyung Jin Sung ◽  
Jessie Sungyun Jeon
Keyword(s):  
Antimicrobial Resistance ◽  
Concentration Gradient ◽  
Acoustic Waves ◽  
Antibiotic Susceptibility ◽  
Pathogenic Bacteria ◽  
Inhibitory Concentration ◽  
Surface Acoustic Waves ◽  
Susceptibility Test ◽  
Antibiotic Susceptibility Test ◽  
Linear Concentration

An efficient and accurate antibiotic susceptibility test (AST) is indispensable for measuring the antimicrobial resistance of pathogenic bacteria. A minimal inhibitory concentration (MIC) can be obtained without performing repeated dilutions...

scholarly journals Bio-activities of Tetracycline and Algae Food Supplement Algo-Bio® on Escherichia coli Antimicrobial Resistance Isolated from Piglet’s Intestinal Flora

2019 ◽  
pp. 1-8
Author(s):  
Amine Naty Tadiogo Kone ◽  
Baguy Mohamed Ouattara ◽  
Bertin Tiekoura ◽  
Fernique Kouadio Konan ◽  
Eugène Koffi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Antibiotic Susceptibility ◽  
Intestinal Flora ◽  
Susceptibility Test ◽  
Diffusion Method ◽  
Bacteriological Study ◽  
Antibiotic Susceptibility Test ◽  
Resistance Rates ◽  
Study Laboratory

Aims: This study aimed at evaluating the effect of Algo-Bio® use on Escherichia coli resistance strains isolated from piglets intestinal flora. Study Design: Bacteriological study. Place and Duration of Study: Laboratory of the National Reference Center for antibiotics at Institute Pasteur Côte d’Ivoire, between March 2018 and June 2018. Methodology: A breeding of three batches of two piglets was carried out, then treatments with tetracycline and Algo-Bio® were administered to them. Enterobacteria was isolated on Mac Conkey medium added up with tetracycline and resistance rates were determined. Escherichia coli resistant strains have been identified and antibiotic susceptibility test was performed using disk diffusion method on Müller-Hinton agar. Results: Enterobacteria resistance rates increased ranging from 18.4% (D0) to 81.5% (D4) to tetracycline-treated piglets and respectively from 25.7% (D0) to 29% (D4) and from 22.3% (D0) to 24.5% (D4), in control piglets and those treated with Algo-Bio®. Antibiotic susceptibility test of Escherichia coli strains isolated from piglets treated with tetracycline showed high resistance to ceftazidime (83.3%), amoxicillin (76.9%) and tetracycline (92.3%) with 39.4% strains producing ESBL, 23.7% producing PHL and 5.2% of producing CHP on D4. Escherichia coli strains isolated from control piglets and Algo-Bio® treated piglets revealed a decrease of ESBL respectively from 17.6% (D0) to 13.7% (D4) and from 12.5% ​​(D0) to 6.4% (D4). Conclusion: The study showed that the use of Algo-Bio® does not induce an evolution of antimicrobial resistance in Microbiota strains and consequently this dietary supplement can be used as a good alternative to antibiotics.

scholarly journals Drug Resistance Associated with Blood Borne Bacteria in Dhaka Metropolis

2019 ◽  
Vol 35 (2) ◽  
pp. 134-140
Author(s):  
Tasnia Ahmed ◽  
Rajib Dey ◽  
Rashed Noor
Keyword(s):  
Antibiotic Susceptibility ◽  
Pathogenic Bacteria ◽  
Healthcare Setting ◽  
Susceptibility Test ◽  
Simple Task ◽  
Blood Samples ◽  
Antibiotic Susceptibility Test ◽  
Wide Range ◽  
Staphylococcus Spp ◽  
New Discovery

Blood borne infections are easy to contract during hospital procedures and can easily be transferred to other personnel in a healthcare setting. Bacteremia has become a common incidence and treatment is by administration of antibiotics. But this is not a simple task any more, since the pathogenic bacteria causing bacteremia are becoming resistant to a wide range of antibiotics. Blood samples were collected from outdoor patients seeking laboratory tests in Dhaka city. Blood samples were inoculated onto blood agar medium and after incubation for 37oC for 24 hours, bacterial isolates were identified and subjected to antibiotic susceptibility test by following the Kirby-Bauer method. The current present study deals with 100 patients of bacteremia from whom 150 isolates of 15 different bacterial genera have been collected among which the most prominent bacteria were Staphylococcus spp. (51 isolates), Pseudomonas spp. (19 isolates) and Escherichia spp. (19 isolates). After the antibiotic susceptibility test it was found that all isolates were resistant to a number of commonly used antibiotics. Twenty eight different antibiotics were used for this study. All isolates showed resistance to CAR, ATM, TOB, CXM, FD, CL, CAZ, AMC. NET and CN showed the most effective results (can effectively clear 9 and 10 types of isolates respectively found in this study). New discovery of drugs to fight these resistant pathogens is needed. In the meantime, safe administration of drugs, handling patients with appropriate protective personal clothing and apparatus, proper waste disposal managements in the hospital should be maintained strictly. Bangladesh J Microbiol, Volume 35 Number 2 December 2018, pp 134-140

Epidemiology, risk factors and antimicrobial resistance of Escherichia coli bacteremia

2021 ◽  
Vol 11 (Number 2) ◽  
pp. 58-67
Author(s):  
Mahjuba Umme Salam ◽  
Selina Yasmin ◽  
Md. Rashedul Haque ◽  
Sharmin Ahmed ◽  
Shahidul Alam ◽  
...  
Keyword(s):  
Risk Factors ◽  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Antibiotic Susceptibility ◽  
Antimicrobial Agents ◽  
Blood Stream Infection ◽  
Incidence Rates ◽  
Diffusion Method ◽  
Antibiotic Susceptibility Test ◽  
Multiple Drugs

Background: Escherichia coli is a common causative of blood stream infection having potentials to produce significant morbidity and mortality. This organism also has the ability to develop resistance against antimicrobial agents. Knowing its epidemiology, risk factors and antimicrobial resistance patterns can help preventing and managing bacteremia caused by this organism. Materials and methods: This was across sectional observational study carried out from February 2017 to February 2018 on 64 blood culture positive Escherichia coli infected patients admitted in Medicine inpatient of a medical college hospital. Age, sex, mode of acquisition of infection, history of prior empiric antibiotic treatment, duration of hospital stay, development of complication were observed and noted. Antibiotic susceptibility test for all isolates was performed by Kirby-Bauer disc diffusion method. Predesigned semi-structured data collection from was used and collected data were analyzed manually and expressed in descriptive statistical terms. Results: Of the 64 enrolled patients, 47(73.43%) were female. Average age of affection was 53.48±20.65 years and increased incidence rates (51.56%) was observed at age >60 years. Infection was communityacquired in 35.84% cases and urinary tract infection was the most frequent (46.3) risk factor. More than eighty seven percent of samples showed resistance to at least one antimicrobial agent and resistance to multiple drugs was associated with complications. Conclusion: Escherichia coli bacteremia has high incidence rates for antimicrobial resistance and mortality. Continuous surveillance and antibiotic susceptibility pattern monitoring is essential to develop regional antibiotic therapy protocols.

scholarly journals Improve antibiotic susceptibility test and its quality control by linear-gradient plate

2017 ◽  
Vol 47 (7) ◽  
pp. 740-749
Author(s):  
Qing ZHANG ◽  
Yin ZHANG ◽  
Jing QI ◽  
YanBo LUO ◽  
LuLu LI ◽  
...  
Keyword(s):  
Quality Control ◽  
Antibiotic Susceptibility ◽  
Susceptibility Test ◽  
Linear Gradient ◽  
Antibiotic Susceptibility Test ◽  
Gradient Plate

Ralstonia insidiosa Neonatal Sepsis: A Case Report and Review of the Literature

2018 ◽  
Vol 15 (03) ◽  
pp. 148-151
Author(s):  
Elçin Akduman Alaşehir ◽  
Belkız Öngen İpek ◽  
David Terence Thomas ◽  
Mustafa Erinç Sitar ◽  
Tuğba Erener Ercan
Keyword(s):  
Intensive Care ◽  
Case Report ◽  
Respiratory Distress ◽  
Antibiotic Susceptibility ◽  
Case Series ◽  
Susceptibility Test ◽  
Test Results ◽  
Presumptive Diagnosis ◽  
Antibiotic Susceptibility Test ◽  
Ralstonia Insidiosa

Introduction Ralstonia spp. are nonfermenting gram-negative bacteria that have recently emerged as opportunistic pathogens. Previously, two case series of infection associated with Ralstonia insidiosa have been published. In this case report, R. insidiosa infection of a neonate in the neonatal intensive care unit (NICU) is presented. Case Presentation A term male infant developed respiratory distress 2 hours after birth and was admitted to the NICU with the presumptive diagnosis of transient tachypnea of the newborn. A left apical pneumothorax was detected, requiring chest tube insertion. An umbilical catheter was placed due to poor peripheral vascular access. On the second day, blood cultures were sent from the umbilical artery and umbilical venous catheters, which showed growth of R. insidiosa. The antibiotics were changed from ampicillin and gentamicin to ampicillin–sulbactam and cefotaxime according to the antibiotic susceptibility test results. Respiratory distress symptoms resolved and the patient was extubated. The infant's clinical condition improved steadily and was discharged with breast feeding and stable vital findings, negative follow-up cultures, and C-reactive protein. Conclusion Ralstonia insidiosa is an emerging pathogen in hospital infections due to its ability to survive in water supplies and sterilized water-based solutions. There is need for vigilance of R. insidiosa, especially in intensive care units. Awareness of rare pathogens, early detection of the bacteria, and antibiotic susceptibility test results are important in the success of treatment.

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