scholarly journals High-throughput optofluidic screening for improved microbial cell factories via real-time micron-scale productivity monitoring

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Matthew Rienzo ◽  
Ke-Chih Lin ◽  
Kellen C. Mobilia ◽  
Eric K. Sackmann ◽  
Volker Kurz ◽  
...  
Keyword(s):  
Steady State ◽  
Real Time ◽  
Batch Culture ◽  
High Throughput ◽  
Real Time Monitoring ◽  
Microbial Cell Factories ◽  
Cell Factories ◽  
Microbial Strains ◽  
Novel Approaches ◽  
Colony Productivity

We describe high-throughput (>103 strains per week) methods for discovery of engineered microbial strains with improved secretion phenotype. These novel approaches use real-time monitoring of colony productivity under steady-state or batch culture.

High-Throughput and Real-Time Monitoring of Single-Cell Extracellular pH Based on Polyaniline Microarrays

2021 ◽  
Author(s):  
Lihui Yang ◽  
Xiaobo Liu ◽  
Bing Yin ◽  
Xunxun Deng ◽  
Xiaotong Lin ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
High Throughput ◽  
Extracellular Ph ◽  
Real Time Monitoring

Real-Time Monitoring of Luciferase-Tagged Cronobacter sakazakii in Reconstituted Infant Milk Formula

2011 ◽  
Vol 74 (4) ◽  
pp. 573-579 ◽  
Author(s):  
RUTH MORRISSEY ◽  
MÁIRE BEGLEY ◽  
SATORU OSHIMA ◽  
MARY REA ◽  
R. PAUL ROSS ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Skim Milk ◽  
Luria Bertani ◽  
Milk Formula ◽  
Cronobacter Sakazakii ◽  
Real Time Monitoring ◽  
Luria Bertani Broth ◽  
Infant Milk Formula

The aim of this study was to examine the potential of using a lux-tagged Cronobacter sakazakii strain to monitor growth of the bacterium in various liquids. C. sakazakii was transformed with plasmid p16Slux, and integration of the plasmid at the desired site on the chromosome was confirmed by PCR. The growth of the lux-tagged strain was similar to that of the non–lux-tagged strain, and the integrated plasmid was stable when cells were cultured in the absence of antibiotic. Growth of the lux-tagged strain was monitored in real time in Luria-Bertani broth, skim milk, and infant milk formula by using both the Luminoskan luminometer and the Xenogen IVIS imager. Bioluminescence could be detected when the lux-tagged strain was cocultured with other bacteria. The effect of monocaprylin and nisin on the growth of C. sakazakii in milk was monitored by measuring bioluminescence. In conclusion, growth of a lux-tagged C. sakazakii can be monitored in real time in both clear and opaque liquids by measuring bioluminescence. lux-tagged C. sakazakii strains could be potentially used in high-throughput assays to monitor the effects of various infant milk formula compositions on growth of the bacterium.

scholarly journals A semi high-throughput method for real-time monitoring of curli producing Salmonella biofilms on air-solid interfaces

Biofilm ◽  
2021 ◽  
pp. 100060
Author(s):  
Ferdinand X. Choong ◽  
Smilla Huzell ◽  
Ming Rosenberg ◽  
Johannes A. Eckert ◽  
Madhu Nagaraj ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Real Time Monitoring ◽  
High Throughput Method

In-situ nanoelectrospray for high-throughput screening of enzymes and real-time monitoring of reactions

2016 ◽  
Vol 902 ◽  
pp. 135-141 ◽  
Author(s):  
Yuhan Yang ◽  
Feifei Han ◽  
Jin Ouyang ◽  
Yunling Zhao ◽  
Juan Han ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
High Throughput Screening ◽  
Real Time Monitoring

scholarly journals An Optical Probe for Real-Time Monitoring of Self-Replicator Emergence and Distinguishing between Replicators

2021 ◽  
Author(s):  
Joydev Hatai ◽  
Yigit Altay ◽  
Ankush Sood ◽  
Armin Kiani ◽  
Marcel Eleveld ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Real Time ◽  
Origin Of Life ◽  
High Throughput ◽  
Optical Probe ◽  
Analysis Time ◽  
Molecular Probe ◽  
Optical Monitoring ◽  
Real Time Monitoring ◽  
High Throughput Experimentation

Self-replicating systems play an important role in research on the synthesis and origin of life. Monitoring of these systems has mostly relied on techniques such as NMR or chromatography, which are limited in throughput and demanding when monitoring replication in real time. To circumvent these problems, we now developed a pattern-generating fluorescent molecular probe (an ID-probe) capable of discriminating replicators of different chemical composition and monitoring the process of replicator formation in real time, giving distinct signatures for starting materials, intermediates and final products. Optical monitoring of replicators dramatically reduces the analysis time and sample quantities compared to most currently used methods and opens the door for future high-throughput experimentation in protocell environments.

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