Slip Formation of a High-Density Droplet Array for Nucleic Acid Quantification by Digital LAMP with a Random Access System

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Weiyuan Lyu ◽  
Jiajie Zhang ◽  
Yan Yu ◽  
Lei Xu ◽  
Feng Shen
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Random Access ◽  
High Density ◽  
Access System ◽  
System P ◽  
Droplet Array ◽  
Precise Quantification ◽  
Nucleic Acid Analysis

Digital nucleic acid analysis (digital NAA) is an important tool for the precise quantification of nucleic acids. Various microfluidic-based approaches for digital NAA have been developed, but most methods either...

Nucleic acids in prion preparations: unspecific background or essential component?

1994 ◽  
Vol 343 (1306) ◽  
pp. 425-430 ◽  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Large Scale ◽  
Size Range ◽  
Complete Recovery ◽  
Tissue Homogenate ◽  
Purification Step ◽  
Prolonged Incubation ◽  
Purification Scheme ◽  
Nucleic Acid Analysis

As recently published (Kellings et al. J. gen Vir. 73, 1025-1029 (1992)), the analysis of purified scrapie prions by return refocusing gel electrophoresis revealed remaining nucleic acids in the size range up to 1100 nucleotides. The results defined the possible characteristics of a hypothetical scrapie-specific nucleic acid. If homogeneous in size, such a molecule would be less than 80 nucleotides in length at a particle-toinfectivity ratio (p: i) near unity; if heterogeneous, scrapie-specific nucleic acids would have to include molecules smaller than 240 nucleotides. To decrease the amount of nucleic acids, several modifications of the PrP Sc purification scheme were introduced. Instead of sucrose gradient, ultrafiltration was applied as a purification step and nucleic acids were degraded by BenzonasetM after ultrafiltration, but significant reduction of the p: i ratio could not be achieved. To prevent trapping of nucleic acids in prion rods, nuclease (Benzonase™ ) was added into the tissue homogenate and incubated at 37°C, overnight. The Benzonase treatment revealed no loss of infectivity, but the whole procedure of nucleic acid analysis did not lead to a reduction of the p :i ratio. In another approach the number of nucleic acid degradations steps was reduced to essentially two steps: Zn 2+ hydrolysis and Benzonase digestion. Higher Zn 2+ concentrations and prolonged incubation times resulted in a more efficient nucleic acid degradation. The bioassays yielded complete recovery of infectivity. Large-scale preparations for determining the p: i ratio are still underway

scholarly journals Noninvasive Prenatal Diagnosis of Fetal Chromosomal Aneuploidies by Maternal Plasma Nucleic Acid Analysis

2008 ◽  
Vol 54 (3) ◽  
pp. 461-466 ◽  
Author(s):  
Y M Dennis Lo ◽  
Rossa W K Chiu
Keyword(s):  
Prenatal Diagnosis ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Maternal Plasma ◽  
Small Sample ◽  
Routine Practice ◽  
Allelic Ratio ◽  
Noninvasive Prenatal Diagnosis ◽  
Nucleic Acid Analysis ◽  
Chromosomal Aneuploidies

Abstract Background: The discovery of circulating cell-free fetal nucleic acids in maternal plasma has opened up new possibilities for noninvasive prenatal diagnosis. The potential application of this technology for the noninvasive prenatal detection of fetal chromosomal aneuploidies is an aspect of this field that is being actively investigated. The main challenge of work in this area is the fact that cell-free fetal nucleic acids represent only a minor fraction of the total nucleic acids in maternal plasma. Methods and Results: We performed a review of the literature, which revealed that investigators have applied methods based on the physical and molecular enrichment of fetal nucleic acid targets from maternal plasma. The former includes the use of size fractionation of plasma DNA and the use of the controversial formaldehyde treatment method. The latter has been achieved through the development of fetal epigenetic and fetal RNA markers. The aneuploidy status of the fetus has been explored through the use of allelic ratio analysis of plasma fetal epigenetic and RNA markers. Digital PCR has been shown to offer high precision for allelic ratio and relative chromosome dosage analyses. Conclusions: After a decade of work, the theoretical and practical feasibility of prenatal fetal chromosomal aneuploidy detection by plasma nucleic acid analysis has been demonstrated in studies using small sample sets. Larger scale independent studies will be needed to validate these initial observations. If these larger scale studies prove successful, it is expected that with further development of new fetal DNA/RNA markers and new analytical methods, molecular noninvasive prenatal diagnosis of the major chromosomal aneuploidies could become a routine practice in the near future.

A highly efficient Baby Spinach-based minimal modified sensor (BSMS) for nucleic acid analysis

2019 ◽  
Vol 17 (30) ◽  
pp. 7222-7227 ◽  
Author(s):  
Rashi Soni ◽  
Deepti Sharma ◽  
A. Murali Krishna ◽  
Jagadeesh Sathiri ◽  
Ashwani Sharma
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
High Selectivity ◽  
Fluorescence Enhancement ◽  
Highly Efficient ◽  
Nucleic Acid Analysis ◽  
Modified Sensor

A Baby Spinach aptamer based minimal-modified sensor (BSMS) detects nucleic acids of potentially any length with high selectivity and specificity, and shows 2.5-fold more fluorescence enhancement compared to the parent aptamer.

A microfabrication-free nanoliter droplet array for nucleic acid detection combined with isothermal amplification

The Analyst ◽  
2015 ◽  
Vol 140 (13) ◽  
pp. 4370-4373 ◽  
Author(s):  
Xiaodong Ma ◽  
Weiwei Xu ◽  
Chao Chen ◽  
Zuhong Lu ◽  
Jiong Li
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Isothermal Amplification ◽  
Nucleic Acid Detection ◽  
Droplet Array ◽  
Microfabrication Technology

A nanoliter droplet array based on a hydrophilic–hydrophobic patterned chip is developed without using microfabrication technology, which could be applied to detect nucleic acids.

pH-Responsive spherical nucleic acid for intracellular lysosome imaging and an effective drug delivery system

2018 ◽  
Vol 54 (28) ◽  
pp. 3520-3523 ◽  
Author(s):  
Hui Li ◽  
Xiang Zhou ◽  
Dongbao Yao ◽  
Haojun Liang
Keyword(s):  
Drug Delivery ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Drug Delivery System ◽  
Delivery System ◽  
Effective Drug ◽  
Ph Responsive ◽  
Spherical Nucleic Acid ◽  
System P ◽  
Spherical Nucleic Acids

This study presents a class of pH-responsive spherical nucleic acids that can exactly image intracellular lysosomes and be an effective drug delivery system.

Nucleic Acid Molecules Prepared by Monolayer Techniques

1972 ◽  
Vol 30 ◽  
pp. 178-179
Author(s):  
Dimitrij Lang
Keyword(s):  
Electron Microscopy ◽  
Standard Deviation ◽  
Nucleic Acid ◽  
Cytochrome C ◽  
Nucleic Acids ◽  
Contour Length ◽  
Sample Standard Deviation ◽  
Molecular Weights ◽  
Length Measurements ◽  
Protein Monolayer

The success of the protein monolayer technique for electron microscopy of individual DNA molecules is based on the prevention of aggregation and orientation of the molecules during drying on specimen grids. DNA adsorbs first to a surface-denatured, insoluble cytochrome c monolayer which is then transferred to grids, without major distortion, by touching. Fig. 1 shows three basic procedures which, modified or not, permit the study of various important properties of nucleic acids, either in concert with other methods or exclusively:1) Molecular weights relative to DNA standards as well as number distributions of molecular weights can be obtained from contour length measurements with a sample standard deviation between 1 and 4%.

Snapshot blotting: The transfer of nucleic acids and nucleoprotein complexes from electrophoresis gels to EM grids

1992 ◽  
Vol 50 (1) ◽  
pp. 762-763
Author(s):  
Stephen D. Jett
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Nucleic Acid Binding ◽  
Mobility Shift ◽  
Carbon Coated ◽  
Nucleoprotein Complexes ◽  
Mobility Shift Assay ◽  
Protein Nucleic Acid ◽  
Gel Mobility Shift ◽  
Nucleic Acid Interactions

The electrophoresis gel mobility shift assay is a popular method for the study of protein-nucleic acid interactions. The binding of proteins to DNA is characterized by a reduction in the electrophoretic mobility of the nucleic acid. Binding affinity, stoichiometry, and kinetics can be obtained from such assays; however, it is often desirable to image the various species in the gel bands using TEM. Present methods for isolation of nucleoproteins from gel bands are inefficient and often destroy the native structure of the complexes. We have developed a technique, called “snapshot blotting,” by which nucleic acids and nucleoprotein complexes in electrophoresis gels can be electrophoretically transferred directly onto carbon-coated grids for TEM imaging.

Molecular insights on the dynamic stability of peptide nucleic acid functionalized carbon and boron nitride nanotubes

2021 ◽  
Vol 23 (1) ◽  
pp. 219-228
Author(s):  
Nabanita Saikia ◽  
Mohamed Taha ◽  
Ravindra Pandey
Keyword(s):  
Nucleic Acid ◽  
Boron Nitride ◽  
Nucleic Acids ◽  
Dynamic Stability ◽  
Peptide Nucleic Acid ◽  
Rational Design ◽  
Peptide Nucleic Acids ◽  
Boron Nitride Nanotubes ◽  
Molecular Hybrids ◽  
Single Wall Nanotubes

The rational design of self-assembled nanobio-molecular hybrids of peptide nucleic acids with single-wall nanotubes rely on understanding how biomolecules recognize and mediate intermolecular interactions with the nanomaterial's surface.

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