scholarly journals Establishment of a human three-dimensional chip-based chondro-synovial co-culture joint model for reciprocal cross-talk studies in arthritis research

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Mario Rothbauer ◽  
Ruth Anne Byrne ◽  
Silvia Schobesberger ◽  
Isabel Olmos Calvo ◽  
Anita Fischer ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Cross Talk ◽  
Synovial Membrane ◽  
Reciprocal Cross ◽  
Three Dimensional ◽  
Joint Model ◽  
Synovial Joint

Rheumatoid arthritis is characterised by a progressive, intermittent inflammation at the synovial membrane, which ultimately leads to the destruction of the synovial joint. The synovial membrane, which is the joint...

scholarly journals Establishment of a human three-dimensional chip-based chondro-synovial co-culture joint model for reciprocal cross-talk studies in arthritis research

2021 ◽  
Author(s):  
Mario Rothbauer ◽  
Ruth A. Byrne ◽  
Silvia Schobesberger ◽  
Isabel Olmos Calvo ◽  
Anita Fischer ◽  
...  
Keyword(s):  
Cross Talk ◽  
Synovial Membrane ◽  
Reciprocal Cross ◽  
Bone Erosion ◽  
Three Dimensional ◽  
Tissue Level ◽  
Cartilage Destruction ◽  
Synovial Joint ◽  
Human Patient

AbstractRheumatoid arthritis is characterised by a progressive, intermittent inflammation at the synovial membrane, which ultimately leads to the destruction of the synovial joint. The synovial membrane, which is the joint capsule’s inner layer, is lined with fibroblast-like synoviocytes that are the key player supporting persistent arthritis leading to bone erosion and cartilage destruction. While microfluidic models that model molecular aspects of bone erosion between bone-derived cells and synoviocytes have been established, RA’s synovial-chondral axis has yet not been realised using a microfluidic 3D model based on human patient in vitro cultures. Consequently, we established a chip-based three-dimensional tissue co-culture model that simulates the reciprocal cross-talk between individual synovial and chondral organoids. We now demonstrate that chondral organoids, when co-cultivated with synovial organoids, induce a higher degree of cartilage physiology and architecture and show differential cytokine response compared to their respective monocultures highlighting the importance of reciprocal tissue-level cross-talk in the modelling of arthritic diseases.

scholarly journals Dosimetric evaluation in radiation synovectomy

2005 ◽  
Vol 48 (spe2) ◽  
pp. 153-158 ◽  
Author(s):  
Carla Flávia de Lima ◽  
Tarcisio Passos Ribeiro de Campo
Keyword(s):  
Rheumatoid Arthritis ◽  
Articular Cartilage ◽  
Synovial Membrane ◽  
Half Life ◽  
Three Dimensional ◽  
Low Energy ◽  
Radioactive Material ◽  
Radiation Synovectomy ◽  
Voxel Model ◽  
The Common

Rheumatoid arthritis can manifest itself through synovitis, of which the knee is the common locale. The treatment using an intra-articular radioisotope injection has been applied in various countries. In this work, the dose of radioactive material absorbed in the joint is evaluated, taking into consideration the dose received in the articular cartilage and adjacencies using a three-dimensional voxel model representing the knee. The radioisotopes studied were Samarium-153 and Dysprosium-165. The results show that the synovial membrane receives 85 to 98% of the normalized dose taken from all voxels representative of the synovium. The following features of 153Sm and of 165Dy - its short physical half-life, the gamma emissions with low energy which allow monitoring the injection trough scintigraphy images, the possibility of binding themselves to macroaggregates that are retained in the joint, the high percentage of the effective dose spread in the synovial membrane - make these suitable radioisotopes for radiation synovectomy.

scholarly journals Oligonucleotide Therapies in the Treatment of Arthritis: A Narrative Review

Biomedicines ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 902
Author(s):  
Susanne N. Wijesinghe ◽  
Mark A. Lindsay ◽  
Simon W. Jones
Keyword(s):  
Rheumatoid Arthritis ◽  
Articular Cartilage ◽  
Joint Diseases ◽  
Synovial Joint ◽  
Pharmacological Treatments ◽  
In Vivo Models ◽  
Inflammatory Joint Diseases ◽  
Joint Pathology ◽  
Cellular Pathways

Osteoarthritis (OA) and rheumatoid arthritis (RA) are two of the most common chronic inflammatory joint diseases, for which there remains a great clinical need to develop safer and more efficacious pharmacological treatments. The pathology of both OA and RA involves multiple tissues within the joint, including the synovial joint lining and the bone, as well as the articular cartilage in OA. In this review, we discuss the potential for the development of oligonucleotide therapies for these disorders by examining the evidence that oligonucleotides can modulate the key cellular pathways that drive the pathology of the inflammatory diseased joint pathology, as well as evidence in preclinical in vivo models that oligonucleotides can modify disease progression.

Are neutrophil extracellular traps the link for the cross-talk between periodontitis and rheumatoid arthritis physiopathology?

Rheumatology ◽  
2021 ◽  
Author(s):  
Sicília Rezende Oliveira ◽  
José Alcides A de Arruda ◽  
Ayda Henriques Schneider ◽  
Valessa Florindo Carvalho ◽  
Caio Machado ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Cross Talk ◽  
Neutrophil Extracellular Traps ◽  
Periodontal Therapy ◽  
Clinical Parameters ◽  
Extracellular Traps ◽  
Early Ra ◽  
The Cross ◽  
High Concentrations ◽  
The Impact

Abstract Objectives Neutrophil extracellular traps (NETs) play a role in the pathogenesis of periodontitis and rheumatoid arthritis (RA). However, it remains poorly understood whether NETs participate in the cross-talk between periodontitis and RA. Herein, we investigated the production of NETs in individuals with periodontitis and RA and its association with clinical parameters. The impact of periodontal therapy on RA and NET release was also assessed. Methods The concentration of NETs and cytokines was determined in the saliva and plasma of individuals with early RA (n = 24), established RA (n = 64), and individuals without RA (n = 76). The influence of periodontitis on the production of NETs and cytokines was also evaluated. Results Individuals with early RA had a higher concentration of NETs in saliva and plasma than individuals with established RA or without RA. Periodontitis resulted in an increase in the concentration of NETs of groups of individuals without RA and with early RA. The proportion of individuals with high concentrations of IL-6, IL-10 and GM-CSF was higher among individuals with periodontitis than among individuals without periodontitis. The concentrations of TNF-α, IL-6, IL-17/IL-25, and IL-28A were particularly high in individuals with early RA. Worse periodontal clinical parameters, RA onset and RA activity were significantly associated with circulating NETs. Periodontal therapy was associated with a reduction in the concentration of NETs and inflammatory cytokines and amelioration in periodontitis and RA. Conclusion This study reveals that NETs are a possible link between periodontitis and RA, with periodontal therapy resulting in a dramatic switch in circulating NET levels.

Distribution of T-cell receptor-bearing lymphocytes in the synovial membrane from patients with rheumatoid arthritis

1990 ◽  
Vol 3 (6) ◽  
pp. 737-745 ◽  
Author(s):  
I CHAOUNI ◽  
M RADAL ◽  
J SIMONYLAFONTAINE ◽  
B COMBE ◽  
J SANY ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
T Cell ◽  
T Cell Receptor ◽  
Synovial Membrane ◽  
Cell Receptor

A three-dimensional nonlinear reduced-order predictive joint model

2003 ◽  
Vol 2 (1) ◽  
pp. 59-73 ◽  
Author(s):  
Yaxin Song ◽  
C. J. Hartwigsen ◽  
Lawrence A. Bergman ◽  
Alexander F. Vakakis
Keyword(s):  
Three Dimensional ◽  
Joint Model ◽  
Reduced Order

Scoring of Synovial Membrane Hypertrophy and Bone Erosions by MR Imaging in Clinically Active and Inactive Rheumatoid Arthritis of the Wrist

1995 ◽  
Vol 24 (4) ◽  
pp. 212-218 ◽  
Author(s):  
M. Østergaard ◽  
P. Gideon ◽  
K. Ssrensen ◽  
M. Hansen ◽  
M. Stoltenberg ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Mr Imaging ◽  
Synovial Membrane ◽  
Bone Erosions
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