Fabrication of monodisperse alginate microgel beads by microfluidic picoinjection: A chelate free approach

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Husnain Ahmed ◽  
Bjørn Torger Stokke
Keyword(s):  
Calcium Alginate ◽  
Biomedical Applications ◽  
Alginate Beads ◽  
Alginate Hydrogel ◽  
Hydrogel Beads ◽  
Ca Alginate

Micron-sized alginate hydrogel beads are extensively employed as an encapsulation medium for biochemical and biomedical applications. Here we report on the microfluidic assisted fabrication of calcium alginate (Ca-alginate) beads by...

scholarly journals Biodegradation of Quinoline by Hydrogel Beads of Activated Sludge in the Air-Lift Bioreactor

2018 ◽  
Vol 7 (4.37) ◽  
pp. 53 ◽  
Author(s):  
Haneen Ahmed Khudhair ◽  
. .
Keyword(s):  
Activated Sludge ◽  
Calcium Alginate ◽  
Biomass Concentration ◽  
Optimal Conditions ◽  
Alginate Hydrogel ◽  
Microbial Cells ◽  
Hydrogel Bead ◽  
Hydrogel Beads ◽  
Air Lift ◽  
Ca Alginate

Quinoline is a nitrogen heterocyclic compound (NHC) with a molecular formula of C9H7N. Microbial degradation of quinoline occurs under both aerobic and anaerobic conditions. In this study, the aerobic biodegradation of quinoline was investigated with activated sludge which was taken from a municipal sewage wastewater treatment plant in the air-lift bioreactor (ALBR).The activated sludge was entrapped in poly-vinyl-alcohol-calcium alginate (PVA-Ca alginate) hydrogel beads. The optimal conditions for microbial cells entrapment, such as Ca-alginate concentration, biomass concentration in the hydrogel bead and bead size, were determined with concerning to improve the quinoline degradation rate. Also, the initial quinoline concentration was determined. The optimum temperature and initial pH for quinoline degradation were 30°C and pH 7–8, respectively. During the biodegradation process, the culture broth became yellow and brown in turn, which indicated that several intermediates were generated. The results showed that the diversity of microbial cells improves and accelerates the quinoline biodegradation. The repeated use of the hydrogel beads for quinoline degradation was performed and the results revealed that the beads were active and intact up to 4 successive cycles without breakage or loss their stability in the continuous mode. Thus, (PVA-Ca alginate) hydrogel beads have great potential to be a matrix for the cell immobilization in quinoline biodegradation. The various initial concentrations of quinoline (50, 100, 250, and 500mg/L) were completely degraded in batch-mode experiments; under optimal conditions: PVA conc., 80%w/v; calcium alginate conc., 20%w/v; initial biomass concentration the hydrogel bead, 3mL / 10 mL of gel solution; and the hydrogel bead size, about 3mm in diameter, at different time intervals (4, 6, 10, and 14h), respectively.   

scholarly journals Alginate hydrogel beads embedded with drug-bearing polycaprolactone microspheres for sustained release of paclobutrazol

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Alexandra Mun ◽  
Haneen Simaan Yameen ◽  
Giora Edelbaum ◽  
Dror Seliktar
Keyword(s):  
Controlled Release ◽  
Biomedical Applications ◽  
Water Immersion ◽  
Alginate Beads ◽  
Model System ◽  
Growth Retardant ◽  
Alginate Hydrogel ◽  
Release Formulation ◽  
Polymeric Systems ◽  
Hydrogel Beads

AbstractIn recent years there has been a growing demand for the development of agrochemical controlled release (CR) technologies. In the present study, we aimed to create a novel agricultural CR device using two polymeric systems that have been predominantly employed in biomedical applications: beads of alginate hydrogel embedded with drug-bearing Polycaprolactone (PCL) microspheres. The combined device utilizes the advantages of each polymer type for biodegradation and controlled release of Paclobutrazol (PBZ), a common growth retardant in plants. Surface morphology of the alginate beads was characterized by scanning electron microscopy (SEM) and water immersion tests were performed for stability and controlled release measurements. Bioassays were performed both in accelerated laboratory conditions and in field conditions. The results showed a capability to control the size of PBZ-loaded PCL microspheres through modification of homogenization speed and emulsifier concentration. Enlargement of PCL microsphere size had an adverse effect on release of PBZ from the alginate device. The growth of oatmeal plants as a model system was affected by the controlled release of PBZ. The preliminary field experiment observed growth retardation during two consecutive rainy seasons, with results indicating a substantial benefit of the sustained growth inhibition through the controlled release formulation. The final product has the potential to be used as a carrier for different substances in the agrochemical industry.

scholarly journals Development of a bioreactor system for the remediation of endosulphan

2018 ◽  
Vol 2 (1) ◽  
pp. 1-8 ◽  
Author(s):  
K. Jesitha ◽  
P. S. Harikumar
Keyword(s):  
Detection Limit ◽  
Calcium Alginate ◽  
Packed Bed ◽  
Alginate Beads ◽  
Soil Samples ◽  
Packed Bed Reactor ◽  
Contaminated Water ◽  
Initial Concentration ◽  
Bioreactor System ◽  
Ca Alginate

Abstract A bioreactor system that consisted of Pseudomonas fluorescens cells immobilised in calcium-alginate beads was utilised to remediate endosulphan contaminated water and soil. A packed bed reactor system was designed for the bio-degradation of endosulphan in artificially spiked water samples (initial concentration of endosulphan: 350 µg/L). Reactor studies with cell-immobilised Ca-alginate beads were conducted after checking their efficiency through batch and column degradation studies. The results showed that the concentration of toxic isomers of endosulphan (endosulphan alpha and endosulphan beta) was below the limit in the bioreactor during the 7th day of the experiment. Experiments conducted with contaminated soil samples (initial concentration of endosulphan: 1,000 μg/kg) indicated that the toxic isomers of endosulphan degraded to below the detection limit within 10 days and monitoring of endosulphan residues on the 14th day revealed that almost complete degradation of metabolites of endosulphan had occurred. The bioreactor system designed can be scaled up for remediation of endosulphan in contaminated areas.

Recovery of gold using graphene oxide/calcium alginate hydrogel beads from a scrap solid state detector

2019 ◽  
Vol 7 (3) ◽  
pp. 103134 ◽  
Author(s):  
Sudeshna Saha ◽  
Manisha Venkatesh ◽  
Hirakendu Basu ◽  
Mehzabin Vivek Pimple ◽  
Rakesh Kumar Singhal
Keyword(s):  
Graphene Oxide ◽  
Solid State ◽  
Calcium Alginate ◽  
Alginate Hydrogel ◽  
Solid State Detector ◽  
Hydrogel Beads ◽  
State Detector ◽  
Calcium Alginate Hydrogel

Separation of no-carrier-added 203Pb, a surrogate radioisotope, from proton irradiated natTl2CO3 target using calcium alginate hydrogel beads

2016 ◽  
Vol 104 (12) ◽  
Author(s):  
Kangkana Sarkar ◽  
Susanta Lahiri ◽  
Kamalika Sen
Keyword(s):  
Calcium Alginate ◽  
Medical Science ◽  
Alginate Hydrogel ◽  
Hydrogel Beads ◽  
Calcium Alginate Hydrogel ◽  
No Carrier Added

AbstractPb is a promising radioisotope in the field of medical science as an imaging surrogate of

scholarly journals Immobilization of Lycinibacillus fusiformis B26 cells in different matrices for use in turquoise blue HFG decolourization

2016 ◽  
Vol 42 (2) ◽  
pp. 92-99 ◽  
Author(s):  
Nazime Mercan Dogan ◽  
Tugba Sensoy ◽  
Gulumser Acar Doganli ◽  
Naime Nur Bozbeyoglu ◽  
Dicle Arar ◽  
...  
Keyword(s):  
Calcium Alginate ◽  
Immobilized Cells ◽  
Alginate Beads ◽  
Optimum Conditions ◽  
Colour Removal ◽  
Operational Conditions ◽  
Cell Pellet ◽  
Cell Concentrations ◽  
Alginate Matrices ◽  
Ca Alginate

Abstract The decolourization of Turquoise Blue HFG by immobilized cells of Lysinibacillus fusiformis B26 was investigated. Cells of L. fusiformis B26 were immobilized by entrapment in agar and calcium alginate matrices and attached in pumice particles. The effects of operational conditions (e.g., agar concentrations, cell concentrations, temperature, and inoculum amount) on microbial decolourization by immobilized cells were investigated. The results revealed that alginate was proven to be the best as exhibiting maximum decolourization (69.62%), followed by agar (55.55%) at 40°C. Pumice particles were the poorest. Optimum conditions for agar matrix were found: concentration was 3%, cell amount was 0.5 g and temperature was 40°C (55.55%). Ca-alginate beads were loaded with 0.5, 1.0 and 2.0 g of wet cell pellets and the highest colour removal activity was observed with 2.0 g of cell pellet at 40°C for alginate beads. Also, 0.5 and 1.0 g of pumice particles that were loaded with 0.25 and 0.5 g of cell pellets respectively were used and the results were found very similar to each other.

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