scholarly journals Routine high-precision Nd isotope analyses: an optimized chromatographic purification scheme

2021 ◽  
Author(s):  
Eugenia Hyung ◽  
François L. H. Tissot
Keyword(s):  
High Precision ◽  
Column Chromatography ◽  
Chromatographic Purification ◽  
Good Separation ◽  
Chromatography Method ◽  
Nd Isotope ◽  
Purification Scheme ◽  
High Yields ◽  
Isotope Analyses

We present an optimized α-HIBA column chromatography method for Nd for high-precision isotope analyses (±2–5 ppm). It produces consistently high yields (>95%) and extremely good separation of Ce, Pr and Sm from Nd.

scholarly journals A Miniaturized Column Chromatography Method for Measuring Receptor-Mediated Inositol Phosphate Accumulation

2004 ◽  
Vol 9 (4) ◽  
pp. 343-353 ◽  
Author(s):  
Elfrida R. Benjamin ◽  
Sarah L. Haftl ◽  
Dimitris N. Xanthos ◽  
Gregg Crumley ◽  
Mohamed Hachicha ◽  
...  
Keyword(s):  
Anion Exchange ◽  
Column Chromatography ◽  
Large Volume ◽  
Inositol Phosphates ◽  
Inositol Phosphate ◽  
Assay Method ◽  
Signal To Noise ◽  
Chromatography Method ◽  
Phosphate Accumulation ◽  
Inositol Phosphate Accumulation

Inositol phosphates (IPs), such as 1,4,5-inositol-trisphosphate (IP3), comprise a ubiquitous intracellular signaling cascade initiated in response to G protein-coupled receptor-mediated activation of phospholipase C. Classical methods for measuring intracellular accumulation of these molecules include time-consuming high-performance liquid chromatography (HPLC) separation or large-volume, gravity-fed anion-exchange column chromatography. More recent approaches, such as radio-receptor and AlphaScreen™ assays, offer higher throughput. However, these techniques rely on measurement of IP3 itself, rather than its accumulation with other downstream IPs, and often suffer from poor signal-to-noise ratios due to the transient nature of IP3. The authors have developed a miniaturized, anion-exchange chromatography method for measuring inositol phosphate accumulation in cells that takes advantage of signal amplification achieved through measuring IP3 and downstream IPs. This assay uses centrifugation of 96-well-formatted anion-exchange mini-columns for the isolation of radiolabeled inositol phosphates from cell extracts, followed by low-background dry-scintillation counting. This improved assay method measures receptor-mediated IP accumulation with signal-to-noise and pharmacological values comparable to the classical large-volume, column-based methods. Assay validation data for recombinant muscarinic receptor 1, galanin receptor 2, and rat astrocyte metabotropic glutamate receptor 5 are presented. This miniaturized protocol reduces reagent usage and assay time as compared to large-column methods and is compatible with standard 96-well scintillation counters.

Review of High-Precision Sr Isotope Analyses of Low-Sr Geological Samples

2015 ◽  
Vol 26 (5) ◽  
pp. 763-774 ◽  
Author(s):  
Jie Lin ◽  
Yongsheng Liu ◽  
Haihong Chen ◽  
Lian Zhou ◽  
Zhaochu Hu ◽  
...  
Keyword(s):  
High Precision ◽  
Geological Samples ◽  
Sr Isotope ◽  
Isotope Analyses

Rb/Sr and Sm/Nd isotope analyses of CCSD eclogites (Sulu, China): A test for the closure temperature concept

2006 ◽  
Vol 70 (18) ◽  
pp. A564
Author(s):  
J. Schneider ◽  
B.-M. Jahn ◽  
K. Okamoto ◽  
L. Tong ◽  
Y. Iizuka ◽  
...  
Keyword(s):  
Closure Temperature ◽  
Nd Isotope ◽  
Temperature Concept ◽  
Isotope Analyses

scholarly journals Amphiphilic Biaryl Monophosphine Ligands by Regioselective Sulfonation

2020 ◽  
Author(s):  
Jacob Rodriguez ◽  
Heemal Dhanjee ◽  
Stephen L. Buchwald
Keyword(s):  
Sulfuric Acid ◽  
Chromatographic Purification ◽  
Organometallic Reactions ◽  
One Step ◽  
High Yields

<div> <p>Amphiphilic ligands are valued for their ability to facilitate organometallic reactions in the presence of water. The regioselective sulfonation of a series of commercially available biaryl monophosphines to generate amphiphilic ligands is presented. In this one-step protocol, the temperature and addition of fuming sulfuric acid were carefully controlled to arrive at sulfonated biaryl monophosphine ligands in high yields with >95% regioselectivity without the need for chromatographic purification.</p> </div>

Direct measurement of Fe isotope compositions in iron-dominate minerals without column chromatography using MC-ICP-MS

2022 ◽  
Author(s):  
Kaiyun Chen ◽  
Zhian Bao ◽  
Honglin Yuan ◽  
Nan Lv
Keyword(s):  
Mass Spectrometry ◽  
Direct Measurement ◽  
High Precision ◽  
Column Chromatography ◽  
Inductively Coupled Plasma ◽  
Practical Method ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry ◽  
Isotope Determination

This study presents a practical method for high-precision Fe isotope determination without column chromatography purification for iron-dominated mineral samples using multi-collector inductively coupled plasma-mass spectrometry (MC-ICP-MS). A series of comparable...

scholarly journals Nd isotope mapping of the Grenvillian Allochthon Boundary Thrust in Algonquin Park, Ontario

2019 ◽  
Vol 56 (2) ◽  
pp. 101-110 ◽  
Author(s):  
A.P. Dickin ◽  
J.W.D. Strong
Keyword(s):  
High Grade ◽  
Geographical Pattern ◽  
Precise Location ◽  
Nd Isotope ◽  
North Shore ◽  
The North ◽  
Digital Elevation ◽  
Elevation Data ◽  
Digital Elevation Data ◽  
Isotope Analyses

Over 50 new Nd isotope analyses are presented for high-grade orthogneisses from Algonquin Park and surrounding region to map major Grenvillian thrust boundaries. Nd model ages display a consistent geographical pattern that allows detailed mapping of the boundary between the Algonquin and Muskoka domains, here interpreted as the local trajectory of the Ottawan-age Allochthon Boundary Thrust (ABT). The ABT is underlain by a domain with Paleoproterozoic Nd model ages, interpreted as a tectonic duplex entrained onto the base of the main allochthon. The boundaries determined using Nd isotope mapping are consistent with field mapping and with remotely sensed aeromagnetic and digital elevation data. The precise location of the ABT can be observed in a road-cut on Highway 60, on the north shore of Lake of Two Rivers in the centre of Algonquin Park.

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