scholarly journals Nanocellulose-based mechanically stable immobilization matrix for enhanced ethylene production: A framework for photosynthetic solid-state cell factories

2021 ◽  
Author(s):  
Ville Rissanen ◽  
Sindhujaa Vajravel ◽  
Sergey Kosourov ◽  
Suvi Arola ◽  
Eero Kontturi ◽  
...  
Keyword(s):  
Solid State ◽  
Ethylene Production ◽  
Cell Immobilization ◽  
Cell Factory ◽  
Cell Factories ◽  
Solid State Cell ◽  
Immobilization Matrix

Cell immobilization is a promising approach to create efficient photosynthetic cell factories for sustainable chemicals production. Here, we demonstrate a novel photosynthetic solid-state cell factory design for sustainable biocatalytic ethylene...

scholarly journals Computational Analysis of Dynamic Light Exposure of Unicellular Algal Cells in a Flat-Panel Photobioreactor to Support Light-Induced CO2 Bioprocess Development

2021 ◽  
Vol 12 ◽  
Author(s):  
Nicolò S. Vasile ◽  
Alessandro Cordara ◽  
Giulia Usai ◽  
Angela Re
Keyword(s):  
Light Transmission ◽  
Light Exposure ◽  
Model Organism ◽  
Cell Factory ◽  
Bioprocess Development ◽  
Cultivation Conditions ◽  
Flat Panel ◽  
Modeling Framework ◽  
Cell Factories ◽  
Cell Trajectories

Cyanobacterial cell factories trace a vibrant pathway to climate change neutrality and sustainable development owing to their ability to turn carbon dioxide-rich waste into a broad portfolio of renewable compounds, which are deemed valuable in green chemistry cross-sectorial applications. Cell factory design requires to define the optimal operational and cultivation conditions. The paramount parameter in biomass cultivation in photobioreactors is the light intensity since it impacts cellular physiology and productivity. Our modeling framework provides a basis for the predictive control of light-limited, light-saturated, and light-inhibited growth of the Synechocystis sp. PCC 6803 model organism in a flat-panel photobioreactor. The model here presented couples computational fluid dynamics, light transmission, kinetic modeling, and the reconstruction of single cell trajectories in differently irradiated areas of the photobioreactor to relate key physiological parameters to the multi-faceted processes occurring in the cultivation environment. Furthermore, our analysis highlights the need for properly constraining the model with decisive qualitative and quantitative data related to light calibration and light measurements both at the inlet and outlet of the photobioreactor in order to boost the accuracy and extrapolation capabilities of the model.

A lithium-iodine solid state cell

1973 ◽  
Vol 18 (2) ◽  
pp. 225-226 ◽  
Author(s):  
B. Scrosati ◽  
M. Torroni
Keyword(s):  
Solid State ◽  
Solid State Cell

Sulfone Containing Clay Electrolytes and Their Potential for Li-Rechargeable Batteries

1998 ◽  
Vol 548 ◽  
Author(s):  
Gregory J. Moore ◽  
M. Stanley Whittingham
Keyword(s):  
Thermal Analysis ◽  
Solid State ◽  
Rechargeable Batteries ◽  
Molecular Formula ◽  
X Ray Diffraction ◽  
Impedance Measurements ◽  
X Ray ◽  
Lithium Secondary Batteries ◽  
Solid State Cell ◽  
Made In

ABSTRACTClays have been synthesized and several types of molecules have been intercalated into them to enhance their ionic conductivity. The clay has the molecular formula of Litaeniolite, Li(Mg2Li)Si4O10F2, and the inserted molecules include PEO and two varieties of sulfone, tetramethylene sulfone and ethylmethyl sulfone. These have been made in the interest of electrolytes in lithium secondary batteries in order to produce a truly solid state cell. The products have been thoroughly characterized by x-ray diffraction to verify the uptake of the molecules into the layers, thermal analysis to observe the stabilization of the intercalated molecules, along with impedance measurements to test their conductivity.

Engineering Frenkel defects of anti-perovskite solid-state electrolytes and their applications in all-solid-state lithium-ion batteries

2020 ◽  
Vol 56 (8) ◽  
pp. 1251-1254 ◽  
Author(s):  
Lihong Yin ◽  
Huimin Yuan ◽  
Long Kong ◽  
Zhouguang Lu ◽  
Yusheng Zhao
Keyword(s):  
Solid State ◽  
Lithium Ion Batteries ◽  
Lithium Ion ◽  
Ion Conductivity ◽  
Cell Configuration ◽  
Solid State Cell ◽  
Frenkel Defects ◽  
Lithium Ion Conductivity ◽  
Solid State Electrolytes

Fluorinated lithium-rich anti-perovskite (F-LiRAP) is proposed to enhance lithium ion conductivity by creating Frenkel defects and an all-solid-state cell configuration based on F-LiRAP is successfully demonstrated.

scholarly journals Intelligent microbial cell factory with genetic pH shooting (GPS) for cell self-responsive base/acid regulation

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Chenyi Li ◽  
Xiaopeng Gao ◽  
Xiao Peng ◽  
Jinlin Li ◽  
Wenxin Bai ◽  
...  
Keyword(s):  
Ph Regulation ◽  
Self Regulation ◽  
Scale Up ◽  
Microbial Cell ◽  
Production Costs ◽  
Cell Factory ◽  
Ph Sensing ◽  
Genetic Circuits ◽  
Microbial Cell Factory ◽  
Cell Factories

Abstract Background In industrial fermentation, pH fluctuation resulted from microbial metabolism influences the strain performance and the final production. The common way to control pH is adding acid or alkali after probe detection, which is not a fine-tuned method and often leads to increased costs and complex downstream processing. Here, we constructed an intelligent pH-sensing and controlling genetic circuits called “Genetic pH Shooting (GPS)” to realize microbial self-regulation of pH. Results In order to achieve the self-regulation of pH, GPS circuits consisting of pH-sensing promoters and acid-/alkali-producing genes were designed and constructed. Designed pH-sensing promoters in the GPS can respond to high or low pHs and generate acidic or alkaline substances, achieving endogenously self-responsive pH adjustments. Base shooting circuit (BSC) and acid shooting circuit (ASC) were constructed and enabled better cell growth under alkaline or acidic conditions, respectively. Furthermore, the genetic circuits including GPS, BSC and ASC were applied to lycopene production with a higher yield without an artificial pH regulation compared with the control under pH values ranging from 5.0 to 9.0. In scale-up fermentations, the lycopene titer in the engineered strain harboring GPS was increased by 137.3% and ammonia usage decreased by 35.6%. Conclusions The pH self-regulation achieved through the GPS circuits is helpful to construct intelligent microbial cell factories and reduce the production costs, which would be much useful in industrial applications.

scholarly journals Enhancement of Astaxanthin Biosynthesis in Oleaginous Yeast Yarrowia lipolytica via Microalgal Pathway

2019 ◽  
Vol 7 (10) ◽  
pp. 472 ◽  
Author(s):  
Larissa Ribeiro Ramos Tramontin ◽  
Kanchana Rueksomtawin Kildegaard ◽  
Suresh Sudarsan ◽  
Irina Borodina
Keyword(s):  
Yarrowia Lipolytica ◽  
Oleaginous Yeast ◽  
Small Scale ◽  
Cell Factory ◽  
Standard Equipment ◽  
Cell Factories ◽  
Dry Cell Weight ◽  
Astaxanthin Production ◽  
Yeast Yarrowia Lipolytica ◽  
Β Carotene

Astaxanthin is a high-value red pigment and antioxidant used by pharmaceutical, cosmetics, and food industries. The astaxanthin produced chemically is costly and is not approved for human consumption due to the presence of by-products. The astaxanthin production by natural microalgae requires large open areas and specialized equipment, the process takes a long time, and results in low titers. Recombinant microbial cell factories can be engineered to produce astaxanthin by fermentation in standard equipment. In this work, an oleaginous yeast Yarrowia lipolytica was engineered to produce astaxanthin at high titers in submerged fermentation. First, a platform strain was created with an optimised pathway towards β-carotene. The platform strain produced 331 ± 66 mg/L of β-carotene in small-scale cultivation, with the cellular content of 2.25% of dry cell weight. Next, the genes encoding β-ketolase and β-hydroxylase of bacterial (Paracoccus sp. and Pantoea ananatis) and algal (Haematococcus pluvialis) origins were introduced into the platform strain in different copy numbers. The resulting strains were screened for astaxanthin production, and the best strain, containing algal β-ketolase and β-hydroxylase, resulted in astaxanthin titer of 44 ± 1 mg/L. The same strain was cultivated in controlled bioreactors, and a titer of 285 ± 19 mg/L of astaxanthin was obtained after seven days of fermentation on complex medium with glucose. Our study shows the potential of Y. lipolytica as the cell factory for astaxanthin production.

The solid state cell

2002 ◽  
Vol 94 (9) ◽  
pp. 555-556 ◽  
Author(s):  
M.A. McNiven
Keyword(s):  
Solid State ◽  
Solid State Cell

Investigation of electrochemical reactions in the solid state cell Cu/RbCu4Cl3I2/C

2001 ◽  
Vol 6 (4) ◽  
pp. 245-252 ◽  
Author(s):  
Gennady I. Ostapenko ◽  
Antony Cox ◽  
Lidiya A. Ostapenko
Keyword(s):  
Solid State ◽  
Electrochemical Reactions ◽  
Solid State Cell
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