scholarly journals Correction: Inhibition of NLRP3 inflammasome activation and pyroptosis with the ethyl acetate fraction of Bungeanum ameliorated cognitive dysfunction in aged mice

2021 ◽  
Author(s):  
Meihuan Zhao ◽  
Yuan Dai ◽  
Ping Li ◽  
Jie Wang ◽  
Tengyun Ma ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Cognitive Dysfunction ◽  
Nlrp3 Inflammasome ◽  
Ethyl Acetate Fraction ◽  
Inflammasome Activation ◽  
Aged Mice ◽  
Nlrp3 Inflammasome Activation

Correction for ‘Inhibition of NLRP3 inflammasome activation and pyroptosis with the ethyl acetate fraction of Bungeanum ameliorated cognitive dysfunction in aged mice’ by Meihuan Zhao et al., Food Funct., 2021, DOI: 10.1039/D1FO00876E.

Inhibition of NLRP3 inflammasome activation and pyroptosis with ethyl acetate fraction of Bungeanum ameliorated cognitive dysfunction in aged mice

2021 ◽  
Author(s):  
Meihuan Zhao ◽  
Yuan Dai ◽  
Ping Li ◽  
Jie wang ◽  
Tengyun Ma ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Cognitive Dysfunction ◽  
Nlrp3 Inflammasome ◽  
Ethyl Acetate Fraction ◽  
Inflammasome Activation ◽  
Material Basis ◽  
Age Related ◽  
Nlrp3 Inflammasome Activation ◽  
Zanthoxylum Bungeanum ◽  
Zanthoxylum Bungeanum Maxim

Zanthoxylum bungeanum Maxim (Rutaceae), a homologous of medicine and foodstuff, has previously been demonstrated the potential prevention of age-related cognitive dysfunction. However, the mechanisms and material basis remain elusively understood....

scholarly journals Gastrodin Alleviates Cognitive Dysfunction and Depressive-Like Behaviors by Inhibiting ER Stress and NLRP3 Inflammasome Activation in db/db Mice

2018 ◽  
Vol 19 (12) ◽  
pp. 3977 ◽  
Author(s):  
Tianyuan Ye ◽  
Xiangbao Meng ◽  
Ruiying Wang ◽  
Chenyang Zhang ◽  
Shuaibing He ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Er Stress ◽  
Learning And Memory ◽  
Cognitive Dysfunction ◽  
Nlrp3 Inflammasome ◽  
Forced Swim Test ◽  
Inflammasome Activation ◽  
Diabetic Encephalopathy ◽  
Gas Treatment ◽  
Nlrp3 Inflammasome Activation

Patients with diabetes mellitus (DM) suffer more risks from diabetic encephalopathy such as cognitive dysfunction and depressive-like behaviors. Numerous studies show that ER (endoplasmic reticulum) stress and inflammation play important roles in the development of diabetic encephalopathy. Gastrodin (Gas), one major component of Gastrodia elata, is traditionally used in central nervous system disorders and is believed to possess anti-inflammatory, anti-apoptotic, and other neuroprotective effects. This present study aims to explore the protective effects of Gas on diabetic encephalopathy. Gas was administrated daily (70 and 140 mg/Kg) for 12 weeks. Meanwhile, the fasting blood glucose and body weight of db/db mice were measured every two weeks. After Gas treatment, the Morris water maze (MWM) test and novel object recognition (NOR) test were performed to assess the learning and memory functions of db/db mice, and the forced swim test was performed to evaluate depressive-like behaviors of db/db mice. Additionally, the expression of ER stress and Nucleotide binding and oligomerization domain-like (Nod) receptor family pyrin domain-containing 3 (NLRP3) inflammasome related proteins were evaluated by using Western blot. Our study suggested that Gas attenuated blood glucose levels and dyslipidemia of db/db mice. It has been shown that Gas could improve learning and memory function and depressive-like behaviors of db/db mice. Moreover, Gas inhibited ER stress and NLRP3 inflammasome activation in the hippocampus. Taken together, this study demonstrates that Gas attenuates the diabetic encephalopathy by inhibiting ER stress and NLRP3 inflammasome activation.

scholarly journals Inhibiting the NLRP3 Inflammasome Activation with MCC950 Ameliorates Diabetic Encephalopathy in db/db Mice

Molecules ◽  
2018 ◽  
Vol 23 (3) ◽  
pp. 522 ◽  
Author(s):  
Yadong Zhai ◽  
Xiangbao Meng ◽  
Tianyuan Ye ◽  
Weijie Xie ◽  
Guibo Sun ◽  
...  
Keyword(s):  
Cognitive Dysfunction ◽  
Nlrp3 Inflammasome ◽  
Cognitive Disorders ◽  
Anxiety And Depression ◽  
Inflammasome Activation ◽  
Diabetic Encephalopathy ◽  
Caspase 1 ◽  
Nlrp3 Inflammasome Activation

Diabetes is associated with a high risk of developing cognitive dysfunction and neuropsychiatric disabilities, and these disease symptomsare termed diabetic encephalopathy (DEP). Inflammation is involved in the development of DEP. The cleavage and maturation of the proinflammatory cytokine interleukin (IL)-1β is regulated by the NLRP3 inflammasome. Obese and type 2 diabetic db/db mice show anxiety- and depression-like behaviors and cognitive disorders associated with hippocampal inflammation. The purpose of this study was to explore the role of NLRP3 inflammasome in DEP. Results showed that expression levels of inflammasome components including NLRP3, apoptosis-associated speck-like protein (ASC), and caspase-1, as well as IL-1β in the hippocampus of diabetic db/db mice were higher than those of non-diabetic db/m mice. Treatment of db/db mice with NLRP3 inflammasome inhibitor MCC950 ameliorated anxiety- and depression-like behaviors as well as cognitive dysfunction, and reversed increased NLRP3, ASC, and IL-1βexpression levels and caspase-1 activity in hippocampus. Moreover, MCC950 treatment significantly improved insulin sensitivity in db/db mice. These results demonstrate that inhibition of NLRP3 inflammasome activation may prove to be a potential therapeutic approach for DEP treatment.

scholarly journals Ethyl acetate extracts of Bungeanum ameliorates cognitive deficits by suppressing NLRP3 inflammasome-dependent pyroptosis in aging mice

2021 ◽  
Author(s):  
Meihuan Zhao ◽  
Yuan Dai ◽  
Ping Li ◽  
Jie Wang ◽  
Tengyun Ma ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Neurodegenerative Disease ◽  
Cognitive Deficits ◽  
Nlrp3 Inflammasome ◽  
Methylene Chloride ◽  
Inflammasome Activation ◽  
Therapeutic Effects ◽  
Active Fraction ◽  
Caspase 1 ◽  
Nlrp3 Inflammasome Activation

Abstract Background: The NLRP3-mediated pyroptosis is a key player in the development of the age-associated neurodegenerative disease. Zanthoxylum bungeanum Maxim (Rutaceae), a homologous of medicine and foodstuff, has previously been demonstrated the potential prevention of cognitive dysfunction in aging mice. However, it is still unknown which fraction is the material basis responsible for their therapeutic effects and whether Z. bungeanum could confer anti-cognitive deficits activity via restraining NLRP3-mediated pyroptosis. Thus, in the current study, we explored the active fraction of Z. bungeanum against cognitive deficits and its underlying mechanism.Methods: In the present study, the D-galactose-induced mouse model of aging was established to explore the effect of cognitive impairment of four fractions of Z. bungeanum, including petroleum ether (PE), methylene chloride (DCM), ethyl acetate (EA), and n-butanol (N-BAI). We next activated the NLRP3 inflammasome in BV-2 microglia cells to investigate the mechanisms for the neuroprotective effect of the active fraction of Z. bungeanum.Results: We demonstrated that the mice treated with EA had significantly alleviated the memory deficit induced by D-galactose. Meanwhile, EA up-regulated the cortex NeuN protein level, improved the survival and morphology of hippocampal neurons. We further found that EA significantly alleviated oxidative damage, inhibited activation of microglia, and suppressed NLRP3 inflammasome activation and subsequent cleaved Caspase-1, IL-1β, IL-18, and GSDMD-N. Correspondingly, in vitro data showed that EA protected BV-2 cells against Lipopolysaccharide (LPS) and adenosine triphosphate (ATP) elicited NLRP3 inflammasome activation and pyroptosis, evidenced by declined the protein levels of NLRP3, cleaved Caspase-1, IL-1β, IL-18, and GSDMD-N. These data indicated that EA inhibited NLRP3-mediated pyroptosis.Conclusions: Our work revealed that the EA active fraction of Z. bungeanum protected neurons from inflammation through inhibition of NLRP3 and subsequent pyroptosis in microglia, and suggested promising clinical use of EA for age-associated neurodegenerative disease.

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