Hypoglycemic effects of Rhodiola crenulata (HK. f. et.Thoms) H. Ohba in vitro and in vivo and its ingredients identification by UPLC-Triple-TOF/MS

2022 ◽  
Author(s):  
Huilan Yue ◽  
Luya Wang ◽  
Sirong Jiang ◽  
Cailang Banma ◽  
Wenjing Jia ◽  
...  
Keyword(s):  
Healthy Food ◽  
Cold Region ◽  
Rhodiola Crenulata ◽  
Tof Ms

Rhodiola crenulata (HK. f. et.Thoms) H. Ohba (RC), mainly distributed in the high cold region in China, has long been used as a medicine/healthy food for eliminating fatigue and increasing...

scholarly journals A novel strategy for the characterization of glaucocalyxin A metabolites in vivo and in vitro by UHPLC-Q-TOF-MS based on DDA and DIA data acquisitions

RSC Advances ◽  
2020 ◽  
Vol 10 (18) ◽  
pp. 10431-10446
Author(s):  
Wenjing Sun ◽  
Yiran Jin ◽  
Shuai Guan ◽  
Mengxin Yang ◽  
Miaoting Zhang ◽  
...  
Keyword(s):  
Process Flow ◽  
Experimental Process ◽  
Novel Strategy ◽  
Tof Ms

The experimental process flow.

Comprehensive characterization of the in vitro and in vivo metabolites of limonin in human samples using LC-Q-TOF/MS

2017 ◽  
Vol 1068-1069 ◽  
pp. 226-232 ◽  
Author(s):  
Shijia Liu ◽  
Peidong Chen ◽  
Nongshan Zhang ◽  
Luning Sun ◽  
Guoliang Dai ◽  
...  
Keyword(s):  
Human Samples ◽  
Comprehensive Characterization ◽  
Tof Ms

scholarly journals A comprehensive study of eriocitrin metabolism in vivo and in vitro based on an efficient UHPLC-Q-TOF-MS/MS strategy

RSC Advances ◽  
2019 ◽  
Vol 9 (43) ◽  
pp. 24963-24980 ◽  
Author(s):  
Luya Li ◽  
Xue Feng ◽  
Yuting Chen ◽  
Shenghao Li ◽  
Yupeng Sun ◽  
...  
Keyword(s):  
Tof Ms ◽  
Comprehensive Study

41 metabolites of eriocitrin in vivo and in vitro was identified based on the efficient UHPLC-Q-TOF-MS/MS strategy.

A Systematic Study of the Metabolites of Dietary Acacetin in Vivo and in Vitro Based on UHPLC-Q-TOF-MS/MS Analysis

2019 ◽  
Vol 67 (19) ◽  
pp. 5530-5543 ◽  
Author(s):  
Jintuo Yin ◽  
Yinling Ma ◽  
Caijuan Liang ◽  
Jin Gao ◽  
Hairong Wang ◽  
...  
Keyword(s):  
Systematic Study ◽  
Ms Analysis ◽  
Tof Ms

scholarly journals Protective Effects of Rhodiola Crenulata Extract on Hypoxia-Induced Endothelial Damage via Regulation of AMPK and ERK Pathways

2018 ◽  
Vol 19 (8) ◽  
pp. 2286 ◽  
Author(s):  
Pi-Kai Chang ◽  
I-Chuan Yen ◽  
Wei-Cheng Tsai ◽  
Tsu-Chung Chang ◽  
Shih-Yu Lee
Keyword(s):  
Oxidative Stress ◽  
Endothelial Cells ◽  
Umbilical Vein ◽  
Hypoxic Exposure ◽  
No Production ◽  
Root Extract ◽  
Protective Effects ◽  
Rhodiola Crenulata

Rhodiola crenulata root extract (RCE) has been shown to possess protective activities against hypoxia both in vitro and in vivo. However, the effects of RCE on response to hypoxia in the endothelium remain unclear. In this study, we aimed to examine the effects of RCE in endothelial cells challenged with hypoxic exposure and to elucidate the underlying mechanisms. Human umbilical vein endothelial cells were pretreated with or without RCE and then exposed to hypoxia (1% O2) for 24 h. Cell viability, nitric oxide (NO) production, oxidative stress markers, as well as mechanistic readouts were studied. We found that hypoxia-induced cell death, impaired NO production, and oxidative stress. These responses were significantly attenuated by RCE treatment and were associated with the activation of AMP-activated kinase and extracellular signal-regulated kinase 1/2 signaling pathways. In summary, we showed that RCE protected endothelial cells from hypoxic insult and suggested that R. crenulata might be useful for the prevention of hypoxia-associated vascular dysfunction.

scholarly journals Pharmacokinetic and Metabolism Studies of Monomethyl Auristatin F via Liquid Chromatography-Quadrupole-Time-of-Flight Mass Spectrometry

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2754
Author(s):  
Park ◽  
Lee ◽  
Byeon ◽  
Shin ◽  
Choi ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Matrix Effects ◽  
Time Of Flight ◽  
Simple Liquid ◽  
Antibody Drug Conjugates ◽  
Drug Conjugates ◽  
Antibody Drug ◽  
Tof Ms

A simple liquid chromatography–quadrupole-time-of-flight–mass spectrometric assay (LC-TOF-MS/MS) has been developed for the evaluation of metabolism and pharmacokinetic (PK) characteristics of monomethyl auristatin F (MMAF) in rat, which is being used as a payload for antibody-drug conjugates. LC-TOF-MS/MS method was qualified for the quantification of MMAF in rat plasma. The calibration curves were acceptable over the concentration range from 3.02 to 2200 ng/mL using quadratic regression. MMAF was stable in various conditions. There were no significant matrix effects between rat and other preclinical species. The PK studies showed that the bioavailability of MMAF was 0% with high clearance. Additionally, the metabolite profiling studies, in vitro/in vivo, were performed. Seven metabolites for MMAF were tentatively identified in liver microsome. The major metabolic pathway was demethylation, which was one of the metabolic pathways predicted by MedChem Designer. Therefore, these results will be helpful to understand the PK, catabolism, and metabolism behavior of MMAF comprehensively when developing antibody-drug conjugates (ADCs) in the future.

LC-Q-TOF/MS-oriented systemic metabolism study of pedunculoside with in vitro and in vivo biotransformation

2019 ◽  
Vol 175 ◽  
pp. 112762 ◽  
Author(s):  
Liang Wu ◽  
An Kang ◽  
Chenxiao Shan ◽  
Chuan Chai ◽  
Zhu Zhou ◽  
...  
Keyword(s):  
Metabolism Study ◽  
Tof Ms
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