Plasmalogens improve swimming performance by modulating the expression of genes involved in amino acids and lipid metabolism, oxidative stress, and ferroptosis in an Alzheimer's disease zebrafish model

2021 ◽  
Author(s):  
Junli Feng ◽  
Gongshuai Song ◽  
Yuanyuan Wu ◽  
Xi Chen ◽  
Jie Pang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Amino Acids ◽  
Lipid Metabolism ◽  
Human Health ◽  
Swimming Performance ◽  
Cognitive Impairments ◽  
Zebrafish Model ◽  
Expression Of Genes ◽  
Health Studies ◽  
Underlying Mechanisms

Plasmalogens (PLs) are critical to human health. Studies have reported a link between downregulation of PLs levels and cognitive impairments in patients with Alzheimer´s disease (AD). however, the underlying mechanisms...

scholarly journals A Discovery of Relevant Hepatoprotective Effects and Underlying Mechanisms of Dietary Clostridium butyricum Against Corticosterone-Induced Liver Injury in Pekin Ducks

2019 ◽  
Vol 7 (9) ◽  
pp. 358 ◽  
Author(s):  
Yanhan Liu ◽  
Cun Liu ◽  
Liqing Huang ◽  
Zhaofei Xia
Keyword(s):  
Oxidative Stress ◽  
Lipid Metabolism ◽  
Liver Injury ◽  
Signaling Pathway ◽  
Basal Diet ◽  
Clostridium Butyricum ◽  
Pekin Ducks ◽  
Injury Model ◽  
Hepatoprotective Effects ◽  
Underlying Mechanisms

Clostridium butyricum (C. butyricum) can attenuate oxidative stress, inflammation, and hepatic fatty deposition in poultry, however, the underlying mechanisms for this in Pekin ducks remain unclear. This study evaluated these hepatoprotective effects and the underlying mechanisms in a corticosterone (CORT)-induced liver injury model in Pekin ducks fed a C. butyricum intervention diet. A total of 500 Pekin ducks were randomly divided into five groups: one group (CON group) was only provided with a basal diet, three groups were provided a basal diet with 200 mg/kg (LCB group), 400 mg/kg (MCB group), or 600 mg/kg (HCB group) C. butyricum, respectively, and one group was provided a basal diet with 150 mg/kg aureomycin (ANT group) for 42 d. At 37 days-old, all ducks received daily intraperitoneal injections of CORT for five days to establish a liver injury model. C. butyricum intervention alleviated liver injury by decreasing the liver organ indices, hepatic steatosis and hepatocyte necrosis, and improving liver function, antioxidant capacity, and inflammatory factors. Hepatic RNA-seq revealed 365 differentially expressed genes (DEGs) between the MCB and CON groups, with 229 up- and 136 down-regulated DEGs in the MCB group. Between the MCB and ANT groups, 407 DEGs were identified, including 299 up- and 108 down-regulated genes in MCB group. Some DEGs in the MCB group related to oxidative stress and inflammatory responses such as Sod3, Tlr2a/b, and Il10, which were up-regulated, while Apoa1, Cyp7a1, Acsl1/5, Fasn, Ppar-γ, and Scd, which are involved in lipid metabolism, were down-regulated, indicating that these genes were responsive to dietary C. butyricum for the alleviation of corticosterone-induced hepatic injury. Toll-like receptor signaling, PI3K-Akt signaling pathway, cytokine-cytokine receptor interaction, peroxisome proliferator-activated receptor (PPAR) signaling pathway, adipocytokine and glycerophospholipid metabolism signaling pathway were significantly enriched in the MCB group. These findings indicate that C. butyricum intervention can protect Pekin ducks from corticosterone-induced liver injury by the modulation of immunoregulatory- and lipid metabolism-related genes and pathways.

scholarly journals Iron Dextran Increases Hepatic Oxidative Stress and Alters Expression of Genes Related to Lipid Metabolism Contributing to Hyperlipidaemia in Murine Model

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Maísa Silva ◽  
Joyce Ferreira da Costa Guerra ◽  
Ana Flávia Santos Sampaio ◽  
Wanderson Geraldo de Lima ◽  
Marcelo Eustáquio Silva ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Metabolism ◽  
Acid Oxidation ◽  
Standard Group ◽  
Mrna Levels ◽  
Iron Dextran ◽  
Iron Stores ◽  
Expression Of Genes ◽  
Carbonyl Protein ◽  
Fischer Rats

The objective of this study was to investigate the effects of iron dextran on lipid metabolism and to determine the involvement of oxidative stress. Fischer rats were divided into two groups: the standard group (S), which was fed the AIN-93M diet, and the standard plus iron group (SI), which was fed the same diet but also received iron dextran injections. Serum cholesterol and triacylglycerol levels were higher in the SI group than in the S group. Iron dextran was associated with decreased mRNA levels ofpparα, and its downstream genecpt1a, which is involved in lipid oxidation. Iron dextran also increased mRNA levels ofapoB-100,MTP, andL-FABPindicating alterations in lipid secretion. Carbonyl protein and TBARS were consistently higher in the liver of the iron-treated rats. Moreover, a significant positive correlation was found between oxidative stress products,lfabpexpression, and iron stores. In addition, a negative correlation was found betweenpparαexpression, TBARS, carbonyl protein, and iron stores. In conclusion, our results suggest that the increase observed in the transport of lipids in the bloodstream and the decreased fatty acid oxidation in rats, which was promoted by iron dextran, might be attributed to increased oxidative stress.

Citrate-capped gold nanoparticles with a diameter of 14nm alter the expression of genes associated with stress response, cytoprotection and lipid metabolism in CaCo-2 cells

2021 ◽  
Author(s):  
Adedoja Dorcas Wusu ◽  
Nicole Remaliah Samantha Sibuyi ◽  
Koena Leah Moabelo ◽  
Mediline Goboza ◽  
Abram Madiehe ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Drug Delivery ◽  
Gold Nanoparticles ◽  
Lipid Metabolism ◽  
Antioxidant Response ◽  
Drug Delivery Vehicles ◽  
Cellular Processes ◽  
Expression Of Genes ◽  
Treatment Conditions ◽  
Delivery Vehicles

Abstract Advancements in nanotechnology have provided insight into the unique opportunities for the application of nanomaterials such as gold nanoparticles (AuNPs) in medicine due to their remarkable properties, which includes low toxicity, large surface area, and the ease of synthesis and conjugation to other molecules. Therefore, AuNPs are often preferred for bio-applications. Citrate-capped AuNPs (cAuNPs) have been reported to be non-cytotoxic and are used in numerous studies as drug delivery vehicles to treat various diseases. However, the limitations of bioassays often used to assess the toxicity of AuNPs have been well documented. Herein, we investigate the cytotoxicity of 14nm cAuNPs in the human colorectal adenocarcinoma (Caco-2) cell line. Treatment conditions (i.e., dose and exposure time) that were established to be non-toxic to Caco-2 cells were used to investigate the effect of cAuNPs on the expression of a Qiagen panel of 86 genes involved in cytotoxicity. Out of 86 studied, 23 genes were differentially expressed. Genes involved in oxidative stress and antioxidant response, endoplasmic reticulum (ER) stress and unfolded protein response (UPR), heat shock response (HSR), and lipid metabolism were more affected than others. While low concentrations of 14nm cAuNPs was not cytotoxic and did not cause cell death, cells treated with these nanoparticles experienced ER and oxidative stress, resulting in the activation of cytoprotective cellular processes. Additionally, several genes involved in lipid metabolism were also affected. Therefore, 14nm cAuNPs can safely be used as drug delivery vehicles at low doses.

scholarly journals Mangifera indica L. Leaf Extract Induces Adiponectin and Regulates Adipogenesis

2019 ◽  
Vol 20 (13) ◽  
pp. 3211 ◽  
Author(s):  
Giuseppe Sferrazzo ◽  
Rosa Palmeri ◽  
Luca Vanella ◽  
Lucia Parafati ◽  
Simone Ronsisvalle ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Metabolic Syndrome ◽  
Lipid Metabolism ◽  
Lipid Content ◽  
Leaf Extract ◽  
Mangifera Indica ◽  
Adipogenic Differentiation ◽  
Obese Patients ◽  
Intracellular Lipid ◽  
Expression Of Genes

Natural bioactive compounds may be used in obese patients because of their ability to impact on various key mechanisms involved in the complex pathophysiological mechanisms of such condition. The aim of this study was to investigate the effect of a Mangifera indica L. leaf extract (MLE) on adipogenic differentiation of murine preadipocyte cells. 3T3-L1 cells were treated during their differentiation with various concentrations of (Mangifera indica L.) leaves extract (MLE) (750, 380, 150, 75 and 35 μg) in order to assess their lipid content, adiponectin production, expression profile of genes involved in lipid metabolism, oxidative stress and inflammation. Our results showed that MLE was particularly enriched in polyphenols (46.30 ± 0.083 mg/g) and that pharmacological treatment of cells resulted in a significant increase of adiponectin levels and reduction of intracellular lipid content. Consistently with these results, MLE resulted in a significant decrease of the expression of genes involved in lipid metabolism (FAS, PPARG, DGAT1, DGAT2, and SCD-1). In conclusion, our results suggest that MLE may represent a possible pharmacological tool for obese or metabolic syndrome patients.

scholarly journals Oral Exposure to Atrazine Induces Oxidative Stress and Calcium Homeostasis Disruption in Spleen of Mice

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Shuying Gao ◽  
Zhichun Wang ◽  
Chonghua Zhang ◽  
Liming Jia ◽  
Yang Zhang
Keyword(s):  
Oxidative Stress ◽  
Calcium Homeostasis ◽  
Protein Product ◽  
Oral Exposure ◽  
Advanced Oxidation Protein Product ◽  
Expression Of Genes ◽  
Intracellular Ca ◽  
Herbicide Atrazine ◽  
Underlying Mechanisms

The widely used herbicide atrazine (ATR) can cause many adverse effects including immunotoxicity, but the underlying mechanisms are not fully understood. The current study investigated the role of oxidative stress and calcium homeostasis in ATR-induced immunotoxicity in mice. ATR at doses of 0, 100, 200, or 400 mg/kg body weight was administered to Balb/c mice daily for 21 days by oral gavage. The studies performed 24 hr after the final exposure showed that ATR could induce the generation of reactive oxygen species in the spleen of the mice, increase the level of advanced oxidation protein product (AOPP) in the host serum, and cause the depletion of reduced glutathione in the serum, each in a dose-related manner. In addition, DNA damage was observed in isolated splenocytes as evidenced by increase in DNA comet tail formation. ATR exposure also caused increases in intracellular Ca2+within splenocytes. Moreover, ATR treatment led to increased expression of genes for some antioxidant enzymes, such asHO-1andGpx1, as well as increased expression of NF-κB and Ref-1 proteins in the spleen. In conclusion, it appears that oxidative stress and disruptions in calcium homeostasis might play an important role in the induction of immunotoxicity in mice by ATR.

scholarly journals TrkB hyperactivity contributes to brain dysconnectivity, epileptogenesis, and anxiety in zebrafish model of Tuberous Sclerosis Complex

2020 ◽  
Vol 117 (4) ◽  
pp. 2170-2179 ◽  
Author(s):  
Magdalena Kedra ◽  
Katarzyna Banasiak ◽  
Katarzyna Kisielewska ◽  
Lidia Wolinska-Niziol ◽  
Jacek Jaworski ◽  
...  
Keyword(s):  
Tuberous Sclerosis ◽  
Tuberous Sclerosis Complex ◽  
Brain Anatomy ◽  
Childhood Epilepsy ◽  
Zebrafish Model ◽  
Cortical Malformations ◽  
Axonal Development ◽  
Expression Of Genes ◽  
Underlying Mechanisms ◽  
Tyrosine Receptor Kinase

Tuberous Sclerosis Complex (TSC) is a rare genetic disease that manifests with early symptoms, including cortical malformations, childhood epilepsy, and TSC-associated neuropsychiatric disorders (TANDs). Cortical malformations arise during embryonic development and have been linked to childhood epilepsy before, but the underlying mechanisms of this relationship remain insufficiently understood. Zebrafish have emerged as a convenient model to study elementary neurodevelopment; however, without in-depth functional analysis, the Tsc2-deficient zebrafish line cannot be used for studies of TANDs or new drug screening. In this study, we found that the lack of Tsc2 in zebrafish resulted in heterotopias and hyperactivation of the mTorC1 pathway in pallial regions, which are homologous to the mammalian cortex. We observed commissural thinning that was responsible for brain dysconnectivity, recapitulating TSC pathology in human patients. The lack of Tsc2 also delayed axonal development and caused aberrant tract fasciculation, corresponding to the abnormal expression of genes involved in axon navigation. The mutants underwent epileptogenesis that resulted in nonmotor seizures and exhibited increased anxiety-like behavior. We further mapped discrete parameters of locomotor activity to epilepsy-like and anxiety-like behaviors, which were rescued by reducing tyrosine receptor kinase B (TrkB) signaling. Moreover, in contrast to treatment with vigabatrin and rapamycin, TrkB inhibition rescued brain dysconnectivity and anxiety-like behavior. These data reveal that commissural thinning results in the aberrant regulation of anxiety, providing a mechanistic link between brain anatomy and human TANDs. Our findings also implicate TrkB signaling in the complex pathology of TSC and reveal a therapeutic target.

scholarly journals Baicalein 5,6-Dimethyl Ether Prevents Memory Deficits in the Scopolamine Zebrafish Model by Regulating Cholinergic and Antioxidant Systems

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1245
Author(s):  
Ion Brinza ◽  
Iriny M. Ayoub ◽  
Omayma A. Eldahshan ◽  
Lucian Hritcu
Keyword(s):  
Oxidative Stress ◽  
Dimethyl Ether ◽  
Exploratory Behavior ◽  
Memory Performance ◽  
Memory Deficits ◽  
Antioxidant Systems ◽  
Zebrafish Model ◽  
Brain Oxidative Stress ◽  
Underlying Mechanisms

Baicalein 5,6-dimethyl ether, a bioactive flavonoid isolated for the first time from Alnus rugosa, was explored for its capability to relieve memory deficits and decrease oxidative stress. We examined the neuropharmacological effects of baicalein 5,6-dimethyl ether on scopolamine (Sco)-induced zebrafish (Danio rerio) anxiety, amnesia, and brain oxidative stress and attempted to elucidate the underlying mechanisms. Anxiety-like behavior, exploratory behavior, and memory performance were measured using novel tank-diving test (NTT), Y-maze, and novel object recognition (NOR) tests. For 10 days, baicalein 5,6-dimethyl ether (1, 3, and 5 µg/L) was administered through immersion, whereas Sco (100 μM) was delivered 30 min before behavioral tests. Treatment with baicalein 5,6-dimethyl ether reduced anxiety and memory impairment, and increased exploratory behavior in specific tests, along with significant protection from neuronal oxidative stress in the brain tissue of Sco-treated zebrafish. Antioxidant and anti-acetylcholinesterase (AChE) activities of baicalein 5,6-dimethyl ether in the Sco-induced zebrafish were further confirmed using in vivo assays. In Sco-treated zebrafish, baicalein 5,6-dimethyl ether regulated cholinergic function by inhibiting AChE activity. Baicalein 5,6-dimethyl ether may be a promising candidate compound for treating anxiety and amnesia by restoring cholinergic activity and reducing brain oxidative stress, according to our findings.

Sign in / Sign up

Export Citation Format

Share Document