Oral administration of EGCG solution equivalent to daily achievable dosages of regular tea drinkers effectively suppresses miR483-3p induced metastasis of hepatocellular carcinoma cells in mice

2021 ◽  
Author(s):  
Qingzheng Kang ◽  
Yin Tong ◽  
Vemana Gowd ◽  
Mingfu Wang ◽  
Feng Chen ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Oral Administration ◽  
Carcinoma Cells ◽  
Human Hepatocellular Carcinoma ◽  
Hepatocellular Carcinoma Cells ◽  
Hcc Cells

The effect of non-cytotoxic doses of epigallocatechin-3-gallate (EGCG) on the metastatic capability of human hepatocellular carcinoma (HCC) cells was investigated in vitro and in vivo. miR483-3p, a microRNA whose expression...

Waltonitone induces human hepatocellular carcinoma cells apoptosis in vitro and in vivo

2009 ◽  
Vol 286 (2) ◽  
pp. 223-231 ◽  
Author(s):  
Zhang Zhang ◽  
Shuo Wang ◽  
Hong Qiu ◽  
Chaohui Duan ◽  
Kan Ding ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Carcinoma Cells ◽  
Human Hepatocellular Carcinoma ◽  
Hepatocellular Carcinoma Cells ◽  
Human Hepatocellular Carcinoma Cells

scholarly journals Anthocyanins Derived from Vitis coignetiae Pulliat Contributes Anti-Cancer Effects by Suppressing NF-κB Pathways in Hep3B Human Hepatocellular Carcinoma Cells and In Vivo

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5445
Author(s):  
Min Jeong Kim ◽  
Anjugam Paramanantham ◽  
Won Sup Lee ◽  
Jeong Won Yun ◽  
Seong Hwan Chang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Carcinoma Cells ◽  
Human Hepatocellular Carcinoma ◽  
Hepatocellular Carcinoma Cells ◽  
In Vivo Experiments ◽  
Anti Cancer ◽  
Hep3b Cells ◽  
Human Hepatocellular Carcinoma Cells

We previously demonstrated that anthocyanins from the fruits of Vitis coignetiae Pulliat (AIMs) induced the apoptosis of hepatocellular carcinoma cells. However, many researchers argued that the concentrations of AIMs were too high for in vivo experiments. Therefore, we performed in vitro at lower concentrations and in vivo experiments for the anti-cancer effects of AIMs. AIMs inhibited the cell proliferation of Hep3B cells in a dose-dependent manner with a maximum concentration of 100 µg/mL. AIMs also inhibited the invasion and migration at 100 µg/mL concentration with or without the presence of TNF-α. To establish the relevance between the in vitro and in vivo results, we validated their effects in a Xenograft model of Hep3B human hepatocellular carcinoma cells. In the in vivo test, AIMs inhibited the tumorigenicity of Hep3B cells in the xenograft mouse model without showing any clinical signs of toxicity or any changes in the body weight of mice. AIMs inhibited the activation NF-κB and suppressed the NF-κB-regulated proteins, intra-tumoral microvessel density (IMVD) and the Ki67 activity of Hep3B xenograft tumors in athymic nude mice. In conclusion, this study indicates that AIMs have anti-cancer effects (inhibition of proliferation, invasion, and angiogenesis) on human hepatocellular carcinoma xenograft through the inhibition of NF-κB and its target protein.

Sign in / Sign up

Export Citation Format

Share Document