Effect of the silkworm pupa protein–glucose conjugate on the thermal stability and antioxidant activity of anthocyanins

2021 ◽  
Author(s):  
Thomas Attaribo ◽  
Gaiqun Huang ◽  
Xiangdong Xin ◽  
Qinlei Zeng ◽  
Yueyue Zhang ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Natural Water ◽  
Water Soluble ◽  
Silkworm Pupa

Anthocyanin (cyanidin-3-O-glucose) is a natural water-soluble pigment with a robust antioxidant capacity.

Antioxidant Capacities of Water-Soluble Substances Extracted from JinHua Ham

2014 ◽  
Vol 675-677 ◽  
pp. 1654-1657
Author(s):  
Xiao Li Zhou ◽  
Xu Chen ◽  
Ting Feng Hao ◽  
Yi Ming Zhou ◽  
Ying Xiao
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Capacity ◽  
Mass Fraction ◽  
Water Soluble ◽  
Oxidation Capacity ◽  
Hydroxyl Free Radical ◽  
Antioxidant Capacities ◽  
Dpph Scavenging ◽  
Jinhua Ham

This study was designed to evaluate the antioxidant activity of samples extracted from Jinhua ham by using such chemical assays as DPPH, scavenging hydroxyl free radical and ABTS. The results demonstrate that antioxidant capacity of the extracted sample is lower than oxidation capacity of Vc. The IC50 spot of samples ranges between 0.6 % and 2.5 % (mass fraction of solute).

scholarly journals Heterosis and antioxidant compounds of sweet corn breeding lines and their F1 hybrid

Genetika ◽  
2014 ◽  
Vol 46 (3) ◽  
pp. 807-813 ◽  
Author(s):  
Boris Kraptchev ◽  
Roumiana Vassilevska-Ivanova ◽  
Lydia Shtereva
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Sweet Corn ◽  
Natural Antioxidants ◽  
Kernel Weight ◽  
Water Soluble ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
F1 Hybrid ◽  
Antioxidants Activity

In this research, the mid-parent heterosis and the levels of phenolic, flavonoids compounds and antioxidants activity among two selected sweet corn parental lines and their F1 hybrid Zaharina were evaluated. Substantial positive mid-parent heterosis (MPH) was found for parent-hybrid triplet for ear weight and for plant height. Among all other traits, insertion height had the greatest heterosis and ear diameter had the lowest. Negative mid-parent heterosis exhibited only the trait 1000-kernel weight. Data analysis indicated significant differences in the contents of total phenolic content, total flavonoids content, water-soluble antioxidant capacity, lipid-soluble antioxidant capacity, and antioxidant activity among parent-hybrid triplet. The results suggest that F1 hybrid Zaharina can be considered as good source of natural antioxidants since it extracts were found to possess high antioxidant activity.

scholarly journals Enhanced Microencapsulation of C-Phycocyanin from Arthrospira by Freeze-Drying with Different Wall Materials

2020 ◽  
Vol 58 (4) ◽  
Author(s):  
Wanida Pan-utai ◽  
Siriluck Iamtham
Keyword(s):  
Thermal Stability ◽  
Antioxidant Activity ◽  
Moisture Content ◽  
Water Activity ◽  
Freeze Drying ◽  
Antioxidant Properties ◽  
Gum Arabic ◽  
Water Soluble ◽  
Freeze Dried ◽  
Wall Materials

Research background. C-phycocyanin (C-PC) as a water-soluble blue pigment was extracted from microalga Arthrospira. C-PC could be a good substitute for synthetic pigments with high antioxidant activity. However, C-PC is unstable due to sensitivity to temperature, light, pH, and oxygen; therefore applications of C-PC in food and other products are limited. Microencapsulation of C-PC using freeze-drying is a solution to this problem and is considered a suitable method for drying heat-sensitive pigment. Experimental approach. C-phycocyanin was extracted from Arthrospira platensis. C-phycocyanin microcapsules were modified by freeze-drying, with different ratios at 0-100 % of maltodextrin (MD) and gum Arabic (GA) used as microencapsulation wall materials. The powders produced were evaluated for physical properties including moisture content and water activity, solubility, hygroscopicity, bulk density, colour appearance, particle morphology and size distribution. Thermal stability and antioxidant activity of freeze-dried C-PC microencapsulated powders were also assessed. Results and conclusions. Freeze-dried C-PC microencapsulated powders with maltodextrin and gum Arabic as wall materials gave high encapsulation efficiency of around 99 %. At higher gum Arabic percentage, moisture content decreased and water activity improved. Maltodextrin gave higher solubility of C-PC powders whereas gum Arabic led to a similar colour of C-PC without microencapsulation. Freeze-dried C-PC microencapsulated powders were composed of different sized microparticles regardless of the combination of wall materials with amorphous glassy shapes. Thermal stability of encapsulated C-PC increased and also showed high antioxidant properties. Novelty and scientific contribution. C-PC microcapsules that maintain colourant stability with high antioxidant levels and resistance to high temperatures can be applied in a wide variety of products and also in the food industry.

scholarly journals Microorganisms and Biostimulants Impact on the Antioxidant Activity of Buckwheat (Fagopyrum esculentum Moench) Sprouts

Antioxidants ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 584 ◽  
Author(s):  
Robert Witkowicz ◽  
Wioletta Biel ◽  
Edyta Skrzypek ◽  
Joanna Chłopicka ◽  
Katarzyna Gleń-Karolczyk ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Water Soluble ◽  
Strongly Correlated ◽  
Growth Promoters ◽  
Pythium Oligandrum ◽  
Antioxidant Power ◽  
Plant Growth Promoters ◽  
Ferric Reducing Antioxidant Power ◽  
Fagopyrum Esculentum Moench

The study analyzes the influence of plant growth promoters and biological control agents on the chemical composition and antioxidant activity (AA) in the sprouts of buckwheat. The AA of cv. Kora sprouts was higher than cv. Panda, with 110.0 µM Fe2+/g (FRAP—Ferric Reducing Antioxidant Power), 52.94 µM TRX (Trolox)/g (DPPH—1,1-diphenyl-2-picrylhydrazyl), 182.7 µM AAE (Ascorbic Acid Equivalent)/g (Photochemiluminescence—PCL—ACW—Water-Soluble Antioxidant Capacity) and 1.250 µM TRX/g (PCL—ACL—Lipid-Soluble Antioxidant Capacity). The highest AA was found in the sprouts grown from seeds soaked in Ecklonia maxima extract and Pythium oligandrum (121.31 µM Fe2+/g (FRAP), 56.33 µM TRX/g (DPPH), 195.6 µM AAE/g (PCL—ACW) and 1.568 µM TRX/g (PCL—ACL). These values show that the antioxidant potential of buckwheat sprouts is essentially due to the predominant hydrophilic fraction of antioxidants. The AA of the sprouts was strongly correlated with total polyphenol content.

Microwave-Assisted Extraction for Maximizing the Yield of Phenolic Compounds and Antioxidant Capacity from Cacao Pod Husk (Theobroma Cacao L.)

2020 ◽  
Vol 16 ◽  
Author(s):  
Van Tang Nguyen ◽  
Thi Dieu Pham ◽  
Long Binh Vu ◽  
Van Hoa Nguyen ◽  
Ngoc Le Tran
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Extraction Efficiency ◽  
Water Soluble ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Phytochemical Compounds

Background: Extraction is an important step to obtain phytochemical compounds from natural materials. Among different extraction techniques, microwave-assisted extraction (MAE) is an advanced method with high extraction efficiency and low energy consumption. Objective: The aim of this study was to optimize the MAE parameters for obtaining the highest levels of phenolic compounds and antioxidant activity from cacao pod husk (CPH). Methods: The dried CPH was prepared using microwave drying method. The CPH extract was prepared using microwave-assisted extraction method. The powdered CPH extract was prepared using vacuum evaporation and freeze drying methods. Phytochemical compounds and antioxidant capacity of CPH extracts were analyzed using spectrophotometric methods. Results: The optimal MAE parameters were 600 W microwave power, 5 s/min irradiation time, 30 min extraction time, and 50 mL/g solvent to sample ratio. Under these MAE parameters, total phenolic content (TPC), phenolic extraction efficiency (PEE), saponin content (SC), and DPPH radical scavenging capacity (DRSC) were achieved from the CPH to be 10.97 mg GAE/g dried sample, 76.82%, 70.10 mg EE/g dried sample, and 141.18 mg DPPH/g dried sample, respectively, which were not significantly different from the predicted values (10.38 mg GAE/g dried sample, 72.68%, 70.09 mg EE/g dried sample, and 121.49 mg DPPH/g dried sample, respectively). The residual moisture, water activity, density, water soluble index and pH of powdered CPH extract were 9.72%, 0.38, 0.17 g/ml, 84.93% and 7.68, respectively. TPC, total flavonoid content (TFC) and SC of powdered CPH extract were 15.75 mg GAE/g dried sample, 27.03 mg CE/g dried sample and 133.67 mg EE/g dried sample, respectively. DRSC and ferric reducing antioxidant power (FRAP) of powdered CPH extract were 227.79 mg DPPH/g dried sample and 14.12 mg Fe(II)/g dried sample, respectively. Conclusion: The findings from this study reveal that the powdered extract obtained from the CPH at the optimal MAE parameters is a rich source of phytochemicals possessing great antioxidant activity. Therefore, it is a potential candidate to apply in the functional foods.

scholarly journals Analysis of Chemical Properties and Antioxidant Activity of Sambiloto (Andrographis paniculata Nees.) Leaf Tea Formula as a Functional Drink in Preventing Coronavirus Diseases and Degenerative Diseases

2021 ◽  
Vol 9 (A) ◽  
pp. 196-201
Author(s):  
Siti Ika Fitrasyah ◽  
Ariani Ariani ◽  
Nurdin Rahman ◽  
Nurulfuadi Nurulfuadi ◽  
Ummu Aiman ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Chemical Properties ◽  
Water Soluble ◽  
National Standard ◽  
Water Extracts ◽  
Ash Alkalinity ◽  
Two Stages ◽  
Functional Drink

AIM: The purpose of this study was to develop a tea formula of sambiloto leaves, analyzed the chemical properties (polyphenols, tannins, water, extracts in water, total ash, water soluble ash, water-insoluble ash, insoluble acid ash, and water-soluble ash alkalinity) as well as the antioxidant capacity of the formula. METHODS: This research was divided into two stages. The first stage was the formulation of sambiloto leaves tea and the second stage was the analysis of chemical properties (levels of polyphenols, tannins, water, extracts water, total ash, water soluble ash, water-insoluble ash, insoluble acid ash, and water soluble ash alkalinity) and antioxidant capacity analysis. The analysis was carried out on all of the sambiloto leaves tea formulas to determine the effect of the formulations on chemical properties and antioxidant capacity. This study used Microsoft Excel 2013 in data processing management. RESULTS: The highest extract water was at F4, and the lowest was at F1. Based on the analysis, the formula of sambiloto leaves tea was in the range of 1.026–1.734%. Of all the formulas that have been prepared, sambiloto leaves tea formula F1 was obtained the best chemical and antioxidant properties among all formulas, which were composed of 100% sambiloto leaves, 5% honey, and 45% dates. CONCLUSION: Sambiloto leaves have good antioxidant activity and can be used as a drink in the form of an infusion, such as tea. The result of brewing the sambiloto tea formula has faded green color. The polyphenol content of the sambiloto leaves tea formula still did not meet the Indonesia National Standard (≥5.2%), was 1.5–3.1%.

EXTRACTION OPTIMIZATION AND ANTIOXIDANT ACTIVITY OF PHENOLIC COMPOUNDS FROM AVOCADO PEEL

2019 ◽  
pp. 49-59
Author(s):  
Nu Linh Giang Ton ◽  
Thi Hoai Nguyen ◽  
Quoc Hung Vo
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Antioxidant Capacity ◽  
Response Surface ◽  
Total Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Quadratic Model ◽  
Total Phenolic ◽  
Total Antioxidant

Avocado peel has been considered as a potential source of natural antioxidants in which phenolics are among the most important compounds. Therefore, this study aims to optimize the extraction process of phenolics using response surface methodology and evaluate the corresponding antioxidant activity. From the quadratic model, the optimal condition was determined including the ethanol concentration 54.55% (v/v), the solvent/solute ratio 71.82/1 (mL/g), temperature 53.03 oC and extraction time 99.09 min. The total phenolic content and the total antioxidant capacity at this condition with minor modifications were 26,74 ± 0,04 (mg GAE/g DW) and 188.06 ± 1.41 (mg AAE/g DW), respectively. The significant correlation between total phenolic content and total antioxidant capacity was also confirmed. Key words: response surface methodology, central composite rotatable design, total phenolic content, total antioxidant capacity, avocado peel

Synthesis, Cytotoxicity and Antioxidant Activity of New Analogs of RC-121 Synthetic Derivatives of Somatostatin

2019 ◽  
Vol 18 (10) ◽  
pp. 1417-1424 ◽  
Author(s):  
Emilia Naydenova ◽  
Diana Wesselinova ◽  
Svetlana Staykova ◽  
Ivan Goshev ◽  
Ljubomir Vezenkov
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colorectal Cancer Cell Line ◽  
Cervical Cancer Cell Line ◽  
Synthetic Derivatives ◽  
Derivatives Of

Background: Based on the structure of RC-121 (D-Phe-c (Cys-Tyr-D-Trp-Lys-Val-Cys)-Thr-NH2, - synthetic derivatives of somatostatin), some analogs were synthesized and tested for in vitro cytotoxic and antioxidant activity. Objectives: The new analogs were modifyed at position 5 with Dap (diaminopropanoic acid), Dab (diaminobutanoic acid) and Orn and at position 6 with the unnatural amino acids Tle (t-leucine). Methods: The in vitro cytotoxic effects of the substances were investigated against a panel of human tumor cell lines HT-29 (Human Colorectal Cancer Cell Line), MDA-MB-23 (Human Breast Cancer Cell Line), Hep G-2 (Human Hepatocellular Carcinoma Cell Line) and HeLa (cervical cancer cell line). The antioxidant capacities were tested by ORAC (Oxygen Radical Antioxidant Capacity) and HORAC (Hydroxyl Radical Averting Capacity) methods. Results: All substances expressed significantly higher antioxidant capacity by comparison with galic acid and Trolox. All substances showed considerable antioxidant capacity as well. Compound 2T (D-Phe-c(Cys-Tyr-DTrp- Dap-Tle-Cys)-Thr-NH2)had the highest antioxidant effect. The compound 4T (D-Phe-c(Cys-Tyr-D-Trp- Orn-Tle-Cys)-Thr-NH2) displayed antiproliferative effect on HeLa cells with IC50 30 µM. The peptide analog 3T (D-Phe-c(Cys-Tyr-D-Trp-Lys-Tle-Cys)-Thr-NH2) exerted the most pronounced inhibition on the cell vitality up to 53%, 56% and 65% resp. against MDA-MB-23, Hep G-2, HeLa in the higher tested concentration. Conclusion: The somatostatin analogs showed moderate influence on the vitality of different tumor cells and could be used in changing their pathology.

Verbascum sinaiticum: A Rich Source of Antioxidant Phenylethanoid Glycosides

2020 ◽  
Vol 10 (2) ◽  
pp. 158-162 ◽  
Author(s):  
Humaira Yasmeen Gondal ◽  
Roshan Zamir ◽  
Muhammad Nisar ◽  
Muhammad Iqbal Choudhary
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Aerial Part ◽  
Methanolic Extract ◽  
Water Soluble ◽  
Antioxidant Potential ◽  
Active Fraction ◽  
Phenylethanoid Glycosides ◽  
Active Constituents ◽  
Soluble Part

Background: The genus Verbascum is well documented for its antioxidant potential but Verbascum sinaiticum is comparatively less studied plant. The current study was carried out to search for antioxidant nutraceuticals from this species. Objective: To explore the antioxidant potential of Verbascum sinaiticum and to identify its active constituents. Methods: The methanolic extract of air-dried aerial part of the Verbascum sinaiticum was partitioned with hexane, chloroform and ethyl acetate. The water-soluble part of ethyl acetate afforded six phenylethanoid glycosides by repeated chromatography over Sephadex LH-20, silica gel and ODS columns. Antioxidant activity of solvent extracts and isolated constituents were evaluated by DPPH, ABTS and FRAP assays. Results: Six phenylethanoid glycosides was isolated and characterized as Verbascoside, Eukovoside, Martynoside, Jionoside D, Campneoside I and Campneoside II, from the most active fraction. Conclusion: Verbascum sinaiticum demonstrated prospective antioxidant activity. The watersoluble part of EtOAc (WSEAE) was found the most active extract whereas Verbascoside was identified as the most potent constituent. All isolated compounds exhibited significant antioxidant activity whereas their synergistic effect was found prominent in the parent fraction.

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