Extraction and Characterization of Matrix Protein from Pacific Oyster（Crassostrea gigas）Shell and its Anti-osteoporosis Properties in vitro and in vivo

2021 ◽  
Author(s):  
Xue Feng ◽  
Suisui Jiang ◽  
Fan Zhang ◽  
Runfang Wang ◽  
Tietao Zhang ◽  
...  
Keyword(s):  
Matrix Protein ◽  
Pacific Oyster ◽  
Secretory Protein ◽  
Water Soluble ◽  
Pacific Oysters ◽  
Bivalve Shell ◽  
P Matrix

Matrix protein is a kind of secretory protein that regulates the biomineralization of bivalve shell. In this study, a water-soluble matrix protein (WSMP) from Pacific oysters (Crassostrea gigs) shell was...

In-silico structural, functional and immunogenic characterization of Taenia solium TS14 protein

2017 ◽  
Author(s):  
Chitra Joshi ◽  
Siddharth Gautam
Keyword(s):  
Amino Acid ◽  
In Silico ◽  
Mutational Analysis ◽  
Solvent Accessibility ◽  
Taenia Solium ◽  
Secretory Protein ◽  
Single Amino Acid

TS14, a Cysticercosis cellulosae derived protein, has been exploited for immunodiagnosis of cysticercosis in humans and pigs. However, the information on structure, function, stability and immunogenicity of TS14 derived from different isolates is primarily lacking. The present study deals with in-silico characterization of six TS14 isolates. High thermostability and an isoelectric point of 9.41 were recorded. Based on N-terminal amino acid residues, high resistance to intracellular proteases with extended in-vivo and in-vitro half-lives was predicted. TS14 is foreseen as a secretory protein with a signal peptide and an extracellular localization. Structural analysis of TS14 exhibited the dominance of helices in the secondary structure (92% coverage) with majority of residues showing high and medium solvent accessibility. High lysine content and presence of multiple nucleotide binding sites in TS14 suggests interaction with RNA/DNA and a role in their metabolism. Immunogenic profiling predicted presence of four distinct B-cell epitopes. Mutational analysis based on the single amino acid substitutions among six TS14 isolates demonstrated minor variations in structural stability; however, all the substitutions were well tolerated. Moreover, all the isolates revealed almost identical immunogenic profile with an equivocal potential to elicit the antibody-mediated immune response.

scholarly journals Extraction, Purification, Bioactivities and Application of Matrix Proteins From Pearl Powder and Nacre Powder: A Review

2021 ◽  
Vol 9 ◽  
Author(s):  
Jingying Pei ◽  
Yan Wang ◽  
Xianguo Zou ◽  
Huajun Ruan ◽  
Changming Tang ◽  
...  
Keyword(s):  
Matrix Protein ◽  
Biological Activities ◽  
Water Soluble ◽  
Isolation And Purification ◽  
Outer Corner ◽  
Extraction And Purification ◽  
Prism Layer ◽  
The Mind

Natural pearls are formed when sand or parasites (irritants) accidentally enter into the oyster body and form pearls under the cover of the nacre layer. Pearl powder is a powdery substance by grinding pearls into small grains, however, the nacre powder is the inner layer of outer corner layer and middle prism layer. Pearl medicine in China has a history of more than 2,000 years, pearl has the effects of calming the mind, clearing the eyes, detoxifying the muscle and so on. In this paper, the researches on the extraction of pearl powder and nacre powder, the isolation and purification of matrix protein and the various biological activities (osteogenic activity, antioxidant, anti-inflammatory, anti-apoptotic, promoting the migration of fibroblasts, and so on) are reviewed in detail. To provide readers with a faster understanding, the method of extraction and purification and the application of nacre powder and pearl powder are clearly presented in the form of figures and tables. In line with the concept of waste or by-product, there are more reports of nacre extract than pearl extract, due to the expensive and limited in origin of pearls. Mainly on the direct use of nacre powder and pearl powder or on the use of extracts (mainly water soluble proteins) through experiments in vivo or in vitro, and shows whether it is effective through the results of various indexes. There is no further study on substances other than extracts, and the structural analysis of extracts needs further exploration.

In Vitro and In Vivo Characterization of a Novel Bifunctionalized Water-Soluble Dithia-Bisphosphine 99mTc Complex

1998 ◽  
Vol 83 (4) ◽  
Author(s):  
R. Schibli ◽  
S. R. Karra ◽  
H. Gali ◽  
Κ. V. Katti ◽  
C. Higginbotham ◽  
...  
Keyword(s):  
Water Soluble ◽  
In Vivo Characterization ◽  
99Mtc Complex

Structural characterization of novel comb-like branched α-d-glucan from Arca inflata and its immunoregulatory activities in vitro and in vivo

2019 ◽  
Vol 10 (10) ◽  
pp. 6589-6603
Author(s):  
Chunlei Li ◽  
Dan Peng ◽  
Weijuan Huang ◽  
Xiaozheng Ou ◽  
Liyan Song ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Water Soluble ◽  
Soluble Polysaccharide

In the current study, we identified and characterized a novel water-soluble polysaccharide (JNY2PW) with significant immunoregulatory effects and no apparent overall toxicity.

In vitro and in vivo characterization of novel water-soluble dithio-bisphosphine 99mTc complexes

1997 ◽  
Vol 24 (7) ◽  
pp. 685-691 ◽  
Author(s):  
C.J. Smith ◽  
N. Li ◽  
K.V. Katti ◽  
C. Higginbotham ◽  
W.A. Volkert
Keyword(s):  
Water Soluble ◽  
In Vivo Characterization

Preparation and characterization of microencapsulated DwPT trivalent vaccine using water soluble chitosan and its in-vitro and in-vivo immunological properties

2018 ◽  
Vol 107 ◽  
pp. 2044-2056 ◽  
Author(s):  
Shilratna Walke ◽  
Gopal Srivastava ◽  
Chinmayee Bar Routaray ◽  
Dilip Dhavale ◽  
Kalpana Pai ◽  
...  
Keyword(s):  
Water Soluble ◽  
Immunological Properties ◽  
Trivalent Vaccine

Rapid, Refined, and Robust Method for Expression, Purification, and Characterization of Recombinant Human Amyloid-beta M1-42

2019 ◽  
Author(s):  
Priya Prakash ◽  
Travis Lantz ◽  
Krupal P. Jethava ◽  
Gaurav Chopra
Keyword(s):  
Amyloid Beta ◽  
Western Blots ◽  
Force Microscopy ◽  
E Coli ◽  
Atomic Force ◽  
Set Up ◽  
Short Time

Amyloid plaques found in the brains of Alzheimer’s disease (AD) patients primarily consists of amyloid beta 1-42 (Ab42). Commercially, Ab42 is synthetized using peptide synthesizers. We describe a robust methodology for expression of recombinant human Ab(M1-42) in Rosetta(DE3)pLysS and BL21(DE3)pLysS competent E. coli with refined and rapid analytical purification techniques. The peptide is isolated and purified from the transformed cells using an optimized set-up for reverse-phase HPLC protocol, using commonly available C18 columns, yielding high amounts of peptide (~15-20 mg per 1 L culture) in a short time. The recombinant Ab(M1-42) forms characteristic aggregates similar to synthetic Ab42 aggregates as verified by western blots and atomic force microscopy to warrant future biological use. Our rapid, refined, and robust technique to purify human Ab(M1-42) can be used to synthesize chemical probes for several downstream in vitro and in vivo assays to facilitate AD research.

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