Isolation and characterization of psychrotolerant denitrifying bacteria for improvement of nitrate removal in woodchip bioreactors treating agricultural drainage water at low temperature

2022 ◽  
Author(s):  
Arnaud Jéglot ◽  
Kirk Matthew Schnorr ◽  
Sebastian Reinhold Sørensen ◽  
Lars Elsgaard
Keyword(s):  
Low Temperature ◽  
Nitrate Removal ◽  
Denitrifying Bacteria ◽  
Drainage Water ◽  
Agricultural Drainage ◽  
Isolation And Characterization ◽  
Agricultural Drainage Water

Nitrate removal was enhanced by the addition of isolated and pre-grown psychrotolerant denitrifiers at low temperature (5 °C).

Nitrate removal potential of restored wetlands loaded with agricultural drainage water: A mesocosm scale experimental approach

2017 ◽  
Vol 106 ◽  
pp. 541-554 ◽  
Author(s):  
Tiffany L. Messer ◽  
Michael R. Burchell ◽  
François Birgand ◽  
Stephen W. Broome ◽  
George Chescheir
Keyword(s):  
Experimental Approach ◽  
Nitrate Removal ◽  
Drainage Water ◽  
Agricultural Drainage ◽  
Restored Wetlands ◽  
Agricultural Drainage Water

scholarly journals Microbiome Structure and Function in Woodchip Bioreactors for Nitrate Removal in Agricultural Drainage Water

2021 ◽  
Vol 12 ◽  
Author(s):  
Arnaud Jéglot ◽  
Joachim Audet ◽  
Sebastian Reinhold Sørensen ◽  
Kirk Schnorr ◽  
Finn Plauborg ◽  
...  
Keyword(s):  
Aquatic Ecosystems ◽  
Nitrate Removal ◽  
Drainage Water ◽  
Agricultural Drainage ◽  
High Similarity ◽  
Agricultural Drainage Water ◽  
Main Pathway ◽  
Genes Encoding ◽  
Denitrification Genes ◽  
And Function

Woodchip bioreactors are increasingly used to remove nitrate (NO3–) from agricultural drainage water in order to protect aquatic ecosystems from excess nitrogen. Nitrate removal in woodchip bioreactors is based on microbial processes, but the microbiomes and their role in bioreactor efficiency are generally poorly characterized. Using metagenomic analyses, we characterized the microbiomes from 3 full-scale bioreactors in Denmark, which had been operating for 4–7 years. The microbiomes were dominated by Proteobacteria and especially the genus Pseudomonas, which is consistent with heterotrophic denitrification as the main pathway of NO3– reduction. This was supported by functional gene analyses, showing the presence of the full suite of denitrification genes from NO3– reductases to nitrous oxide reductases. Genes encoding for dissimilatory NO3– reduction to ammonium were found only in minor proportions. In addition to NO3– reducers, the bioreactors harbored distinct functional groups, such as lignocellulose degrading fungi and bacteria, dissimilatory sulfate reducers and methanogens. Further, all bioreactors harbored genera of heterotrophic iron reducers and anaerobic iron oxidizers (Acidovorax) indicating a potential for iron-mediated denitrification. Ecological indices of species diversity showed high similarity between the bioreactors and between the different positions along the flow path, indicating that the woodchip resource niche was important in shaping the microbiome. This trait may be favorable for the development of common microbiological strategies to increase the NO3– removal from agricultural drainage water.

scholarly journals Temperature Sensitivity and Composition of Nitrate-Reducing Microbiomes from a Full-Scale Woodchip Bioreactor Treating Agricultural Drainage Water

2021 ◽  
Vol 9 (6) ◽  
pp. 1331
Author(s):  
Arnaud Jéglot ◽  
Sebastian Reinhold Sørensen ◽  
Kirk M. Schnorr ◽  
Finn Plauborg ◽  
Lars Elsgaard
Keyword(s):  
Temperature Response ◽  
Microbial Transformation ◽  
Drainage Water ◽  
Microbial Populations ◽  
Agricultural Drainage ◽  
Bacterial Populations ◽  
Cold Temperatures ◽  
Cold Adapted ◽  
Agricultural Drainage Water ◽  
Temperature Responses

Denitrifying woodchip bioreactors (WBR), which aim to reduce nitrate (NO3−) pollution from agricultural drainage water, are less efficient when cold temperatures slow down the microbial transformation processes. Conducting bioaugmentation could potentially increase the NO3− removal efficiency during these specific periods. First, it is necessary to investigate denitrifying microbial populations in these facilities and understand their temperature responses. We hypothesized that seasonal changes and subsequent adaptations of microbial populations would allow for enrichment of cold-adapted denitrifying bacterial populations with potential use for bioaugmentation. Woodchip material was sampled from an operating WBR during spring, fall, and winter and used for enrichments of denitrifiers that were characterized by studies of metagenomics and temperature dependence of NO3− depletion. The successful enrichment of psychrotolerant denitrifiers was supported by the differences in temperature response, with the apparent domination of the phylum Proteobacteria and the genus Pseudomonas. The enrichments were found to have different microbiomes’ composition and they mainly differed with native woodchip microbiomes by a lower abundance of the genus Flavobacterium. Overall, the performance and composition of the enriched denitrifying population from the WBR microbiome indicated a potential for efficient NO3− removal at cold temperatures that could be stimulated by the addition of selected cold-adapted denitrifying bacteria.

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