Electrochemistry-coupled to liquid chromatography-mass spectrometry-density functional theory as a new tool to mimic the environmental degradation of selected phenylurea herbicides

2021 ◽  
Author(s):  
Ranil Clément Tonleu Temgoua ◽  
Ugo Bussy ◽  
Dimitri Alvarez-Dorta ◽  
Nicolas Galland ◽  
Evangeline Njanja ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Metabolic Pathways ◽  
Density Functional ◽  
Experimental Models ◽  
Liquid Chromatography Mass Spectrometry ◽  
Phenylurea Herbicides ◽  
Functional Theory ◽  
Healthy Humans

In vitro and in vivo experimental models, mainly based on cell cultures, animals, healthy humans and clinical trials, are useful approaches for identifying the main metabolic pathways.

scholarly journals Placental 13C-DHA metabolism and relationship with maternal BMI, glycemia and birthweight

2021 ◽  
Vol 27 (1) ◽  
Author(s):  
Oliver C. Watkins ◽  
Preben Selvam ◽  
Reshma Appukuttan Pillai ◽  
Victoria K. B. Cracknell-Hazra ◽  
Hannah E. J. Yong ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Lipid Metabolism ◽  
Metabolic Pathways ◽  
Liquid Chromatography Mass Spectrometry ◽  
Fasting Glycemia ◽  
Glucose Treatment ◽  
Maternal Bmi

Abstract Background Fetal docosahexaenoic acid (DHA) supply relies on preferential transplacental transfer, which is regulated by placental DHA lipid metabolism. Maternal hyperglycemia and obesity associate with higher birthweight and fetal DHA insufficiency but the role of placental DHA metabolism is unclear. Methods Explants from 17 term placenta were incubated with 13C-labeled DHA for 48 h, at 5 or 10 mmol/L glucose treatment, and the production of 17 individual newly synthesized 13C-DHA labeled lipids quantified by liquid chromatography mass spectrometry. Results Maternal BMI positively associated with 13C-DHA-labeled diacylglycerols, triacylglycerols, lysophospholipids, phosphatidylcholine and phosphatidylethanolamine plasmalogens, while maternal fasting glycemia positively associated with five 13C-DHA triacylglycerols. In turn, 13C-DHA-labeled phospholipids and triacylglycerols positively associated with birthweight centile. In-vitro glucose treatment increased most 13C-DHA-lipids, but decreased 13C-DHA phosphatidylethanolamine plasmalogens. However, with increasing maternal BMI, the magnitude of the glucose treatment induced increase in 13C-DHA phosphatidylcholine and 13C-DHA lysophospholipids was curtailed, with further decline in 13C-DHA phosphatidylethanolamine plasmalogens. Conversely, with increasing birthweight centile glucose treatment induced increases in 13C-DHA triacylglycerols were exaggerated, while glucose treatment induced decreases in 13C-DHA phosphatidylethanolamine plasmalogens were diminished. Conclusions Maternal BMI and glycemia increased the production of different placental DHA lipids implying impact on different metabolic pathways. Glucose-induced elevation in placental DHA metabolism is moderated with higher maternal BMI. In turn, findings of associations between many DHA lipids with birthweight suggest that BMI and glycemia promote fetal growth partly through changes in placental DHA metabolism.

Gut microbiota-mediated deglycosylation of ginsenoside Rb1 in rats: in vitro and in vivo insights from quantitative ultra-performance liquid chromatography-mass spectrometry analysis

2015 ◽  
Vol 7 (15) ◽  
pp. 6173-6181 ◽  
Author(s):  
Kang An ◽  
Zhang Shengjie ◽  
Shan Jinjun ◽  
Di Liuqing
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Biological Activities ◽  
Ultra Performance Liquid Chromatography ◽  
Mass Spectrometry Analysis ◽  
Ginsenoside Rb1 ◽  
Liquid Chromatography Mass Spectrometry ◽  
Spectrometry Analysis

Ginsenoside Rb1, an ingredient of the herbal medicine Panax ginseng, possesses a variety of biological activities.

scholarly journals Localization of sunitinib in in vivo animal and in vitro experimental models by MALDI mass spectrometry imaging

2014 ◽  
Vol 407 (8) ◽  
pp. 2245-2253 ◽  
Author(s):  
James J. Connell ◽  
Yutaka Sugihara ◽  
Szilvia Török ◽  
Balázs Döme ◽  
József Tóvári ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Maldi Mass Spectrometry ◽  
Experimental Models ◽  
Maldi Mass Spectrometry Imaging

Identification of in vitro and in vivo metabolites of isoimperatorin using liquid chromatography/mass spectrometry

2013 ◽  
Vol 141 (1) ◽  
pp. 357-365 ◽  
Author(s):  
Xiaowei Shi ◽  
Man Liu ◽  
Min Zhang ◽  
Kerong Zhang ◽  
Songchen Liu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry
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