Photo-isomerization of the isolated photoactive yellow protein chromophore: What comes before the primary step?

2021 ◽  
Author(s):  
Cate S Anstöter ◽  
Basile Curchod ◽  
Jan RR Verlet
Keyword(s):  
Structural Changes ◽  
Biological Response ◽  
Photoactive Yellow Protein

Photoactive proteins typically rely on structural changes in a small chromophore to initiate a biological response. While these changes often involve isomerization as the “primary step”, preceding this is an...

Binding of Hydrogen-Citrate to Photoactive Yellow Protein Is Affected by the Structural Changes Related to Signaling State Formation

2012 ◽  
Vol 116 (44) ◽  
pp. 13172-13182 ◽  
Author(s):  
Marijke Hospes ◽  
Johannes H. Ippel ◽  
Rolf Boelens ◽  
Klaas J. Hellingwerf ◽  
Johnny Hendriks
Keyword(s):  
State Formation ◽  
Structural Changes ◽  
Photoactive Yellow Protein

scholarly journals Comparison of the Photochemical Reaction of Photoactive Yellow Protein in Crystal with Reaction in Solution1

2003 ◽  
Vol 17 (2-3) ◽  
pp. 345-353 ◽  
Author(s):  
Eriko Mano ◽  
Hironari Kamikubo ◽  
Yasushi Imamoto ◽  
Mikio Kataoka
Keyword(s):  
Photochemical Reaction ◽  
Structural Changes ◽  
Spectroscopic Studies ◽  
Crystalline Lattice ◽  
Photoactive Yellow Protein ◽  
X Ray ◽  
X Ray Crystallography ◽  
Solution Condition ◽  
Active Intermediate ◽  
Ectothiorhodospira Halophila

Photoactive yellow protein (PYP) is a photoreceptor protein for the negative phototaxis ofEctothiorhodospira halophila. The crystal structures of several photo‒intermediates have been revealed by X-ray crystallography. In the crystal structure of the active intermediate, PYPM, no significant structural changes were observed except for the vicinity of the chromophore. On the contrary, spectroscopic studies with solution condition demonstrated that global structural changes occur during the photo‒cycle. In order to reveal the origin of the discrepancies, we measured the reaction kinetics upon illumination under crystal condition and to compare them with those observed under solution condition. The reactive portion decreases with the increase of crystallinity. The rate constant of PYPMdecay also decreases with the increase of crystallinity. These results suggest two possibilities: (1) PYP in crystal does not react by the illumination; (2) the photoreaction rate is highly accelerated in crystal. Consequently, the photoreaction in crystal is considered to be highly influenced by the force constraint from crystalline lattice.

scholarly journals Chromophore Conformation and the Evolution of Tertiary Structural Changes in Photoactive Yellow Protein

Structure ◽  
2004 ◽  
Vol 12 (6) ◽  
pp. 1039-1045 ◽  
Author(s):  
Spencer Anderson ◽  
Vukica Srajer ◽  
Reinhard Pahl ◽  
Sudarshan Rajagopal ◽  
Friedrich Schotte ◽  
...  
Keyword(s):  
Structural Changes ◽  
Photoactive Yellow Protein

scholarly journals The use of optical coherence tomography envelope statistics to quantify local temperature changes in tissue-mimicking phantoms

2021 ◽  
Author(s):  
Subaagari Seevaratnam
Keyword(s):  
Optical Coherence Tomography ◽  
Structural Changes ◽  
Structural Information ◽  
Malignant Tumors ◽  
Biological Response ◽  
Improve Patient Care ◽  
Distribution Functions ◽  
Optical Coherence ◽  
Temperature Changes ◽  
Generalized Gamma Function

There exists a multitude of extreme thermally induced therapies for treating both benign and malignant tumors. Advancement in optics has shown prospect in clinical applications, especially monitoring oncological clinical treatments like thermal ablation. Optical coherence tomography (OCT) backscattered spectrum has demonstrated sensitivity to structural changes on the microscopic level. Envelope statistics analysis on OCT images of tissue-mimicking phantoms that are thermally modulated can provide structural information that correlates to changes in temperature. Several probability distribution functions were analyzed by looking at suitable theoretical matches to the empirical OCT data. Results indicate that the generalized gamma function was the best fit and has potential in relating the scale parameter to the size of the scatterers in the phantom. Moreover, the parameters revealed sensitivity to temperature changes, which can be further studied to understand the biological response of tissue that are exposed to extreme thermal conditions in order to improve patient care.

scholarly journals The use of optical coherence tomography envelope statistics to quantify local temperature changes in tissue-mimicking phantoms

2021 ◽  
Author(s):  
Subaagari Seevaratnam
Keyword(s):  
Optical Coherence Tomography ◽  
Structural Changes ◽  
Structural Information ◽  
Malignant Tumors ◽  
Biological Response ◽  
Improve Patient Care ◽  
Distribution Functions ◽  
Optical Coherence ◽  
Temperature Changes ◽  
Generalized Gamma Function

There exists a multitude of extreme thermally induced therapies for treating both benign and malignant tumors. Advancement in optics has shown prospect in clinical applications, especially monitoring oncological clinical treatments like thermal ablation. Optical coherence tomography (OCT) backscattered spectrum has demonstrated sensitivity to structural changes on the microscopic level. Envelope statistics analysis on OCT images of tissue-mimicking phantoms that are thermally modulated can provide structural information that correlates to changes in temperature. Several probability distribution functions were analyzed by looking at suitable theoretical matches to the empirical OCT data. Results indicate that the generalized gamma function was the best fit and has potential in relating the scale parameter to the size of the scatterers in the phantom. Moreover, the parameters revealed sensitivity to temperature changes, which can be further studied to understand the biological response of tissue that are exposed to extreme thermal conditions in order to improve patient care.

scholarly journals Dynamics of Protein and Chromophore Structural Changes in the Photocycle of Photoactive Yellow Protein Monitored by Time-Resolved Optical Rotatory Dispersion†,‡

Biochemistry ◽  
2003 ◽  
Vol 42 (7) ◽  
pp. 2062-2071 ◽  
Author(s):  
Eefei Chen ◽  
Thomas Gensch ◽  
Andrew B. Gross ◽  
Johnny Hendriks ◽  
Klaas J. Hellingwerf ◽  
...  
Keyword(s):  
Structural Changes ◽  
Optical Rotatory Dispersion ◽  
Rotatory Dispersion ◽  
Photoactive Yellow Protein ◽  
Optical Rotatory ◽  
Time Resolved

scholarly journals 1P280 Protein Structural Changes during the formation of M Intermediate in Photoactive Yellow Protein : Ultraviolet Resonance Raman Study

2004 ◽  
Vol 44 (supplement) ◽  
pp. S99
Author(s):  
M. Unno ◽  
Samir F. El-Mashotoly ◽  
M. Kumauchi ◽  
N. Hamada ◽  
F. Tokunaga ◽  
...  
Keyword(s):  
Structural Changes ◽  
Resonance Raman ◽  
Photoactive Yellow Protein ◽  
Raman Study ◽  
Ultraviolet Resonance Raman
Sign in / Sign up

Export Citation Format

Share Document