scholarly journals Interactions in protein solutions close to liquid-liquid phase separation: Ethanol reduces attractions via changes of the dielectric solution properties

2021 ◽  
Author(s):  
Jan Hansen ◽  
Rajeevann Uthayakumar ◽  
Jan Skov Pedersen ◽  
Stefan U. Egelhaaf ◽  
Florian Platten
Keyword(s):  
Phase Separation ◽  
Dielectric Properties ◽  
Liquid Phase ◽  
Protein Crystallization ◽  
Liquid Phase Separation ◽  
Protein Solutions ◽  
Solution Properties ◽  
Liquid Liquid Phase Separation

Ethanol is a common protein crystallization agent, precipitant, and denaturant, but also alters the dielectric properties of solutions. While ethanol-induced unfolding is largely ascribed to its hydrophobic parts, its effect...

Reentrant condensation, liquid–liquid phase separation and crystallization in protein solutions induced by multivalent metal ions

2014 ◽  
Vol 86 (2) ◽  
pp. 191-202 ◽  
Author(s):  
Fajun Zhang ◽  
Felix Roosen-Runge ◽  
Andrea Sauter ◽  
Marcell Wolf ◽  
Robert M. J. Jacobs ◽  
...  
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Metal Ions ◽  
Protein Interactions ◽  
Protein Crystallization ◽  
Liquid Phase Separation ◽  
Protein Solutions ◽  
Metastable Liquid ◽  
Multivalent Metal ◽  
Liquid Liquid Phase Separation

Abstract We briefly summarize the recent progress in tuning protein interactions as well as phase behavior in protein solutions using multivalent metal ions. We focus on the influence of control parameters and the mechanism of reentrant condensation, the metastable liquid–liquid phase separation and classical vs. non-classical pathways of protein crystallization.

Charge-controlled metastable liquid–liquid phase separation in protein solutions as a universal pathway towards crystallization

Soft Matter ◽  
2012 ◽  
Vol 8 (5) ◽  
pp. 1313-1316 ◽  
Author(s):  
Fajun Zhang ◽  
Roland Roth ◽  
Marcell Wolf ◽  
Felix Roosen-Runge ◽  
Maximilian W. A. Skoda ◽  
...  
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Liquid Phase Separation ◽  
Protein Solutions ◽  
Metastable Liquid ◽  
Liquid Liquid Phase Separation

scholarly journals Kinetics of liquid–liquid phase separation in protein solutions exhibiting LCST phase behavior studied by time-resolved USAXS and VSANS

Soft Matter ◽  
2016 ◽  
Vol 12 (46) ◽  
pp. 9334-9341 ◽  
Author(s):  
Stefano Da Vela ◽  
Michal K. Braun ◽  
Andreas Dörr ◽  
Alessandro Greco ◽  
Johannes Möller ◽  
...  
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Phase Behavior ◽  
Liquid Phase Separation ◽  
Protein Solutions ◽  
Time Resolved ◽  
Kinetics Of ◽  
Liquid Liquid Phase Separation

The role of cluster formation and metastable liquid—liquid phase separation in protein crystallization

2012 ◽  
Vol 159 ◽  
pp. 313 ◽  
Author(s):  
Fajun Zhang ◽  
Felix Roosen-Runge ◽  
Andrea Sauter ◽  
Roland Roth ◽  
Maximilian W. A. Skoda ◽  
...  
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Protein Crystallization ◽  
Cluster Formation ◽  
Liquid Phase Separation ◽  
Metastable Liquid ◽  
Liquid Liquid Phase Separation

Protein Crystallization in the Presence of a Metastable Liquid–Liquid Phase Separation

2020 ◽  
Vol 20 (12) ◽  
pp. 7951-7962
Author(s):  
Ralph Maier ◽  
Georg Zocher ◽  
Andrea Sauter ◽  
Stefano Da Vela ◽  
Olga Matsarskaia ◽  
...  
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Protein Crystallization ◽  
Liquid Phase Separation ◽  
Metastable Liquid ◽  
Liquid Liquid Phase Separation

Reentrant Liquid-Liquid Phase Separation in Protein Solutions at Elevated Hydrostatic Pressures

2014 ◽  
Vol 112 (2) ◽  
Author(s):  
Johannes Möller ◽  
Sebastian Grobelny ◽  
Julian Schulze ◽  
Steffen Bieder ◽  
Andre Steffen ◽  
...  
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Liquid Phase Separation ◽  
Protein Solutions ◽  
Liquid Liquid Phase Separation
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