Three-step Fӧrster resonance energy transfer on amyloid fibril scaffold

2021 ◽  
Author(s):  
Galyna Gorbenko ◽  
Olga Zhytniakivska ◽  
Kateryna Vus ◽  
Uliana Tarabara ◽  
Valeriya Trusova
Keyword(s):  
Energy Transfer ◽  
Amyloid Fibril ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Thioflavin T ◽  
Squaraine Dye ◽  
Transfer Chain

The present study provides evidence that the energy transfer chain consisting of the benzothiazole dye Thioflavin T as an input donor, a phosphonium dye TDV and a squaraine dye SQ4...

Fӧrster resonance energy transfer between Thioflavin T and unsymmetrical trimethine cyanine dyes on amyloid fibril scaffold

2021 ◽  
pp. 139127
Author(s):  
Olga Zhytniakivska ◽  
Atanas Kurutos ◽  
Mykhailo Shchuka ◽  
Kateryna Vus ◽  
Uliana Tarabara ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Amyloid Fibril ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Cyanine Dyes ◽  
Thioflavin T

Interaction of Thioflavin T with amyloid fibrils of apolipoprotein A-I N-terminal fragment: Resonance energy transfer study

2014 ◽  
Vol 185 (1) ◽  
pp. 116-124 ◽  
Author(s):  
Mykhailo Girych ◽  
Galyna Gorbenko ◽  
Valeriya Trusova ◽  
Emi Adachi ◽  
Chiharu Mizuguchi ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Amyloid Fibrils ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Thioflavin T ◽  
Apolipoprotein A ◽  
Terminal Fragment ◽  
Transfer Study

Quantum Dot Bioconjugates as Energy Donors in Fluorescence Resonance Energy Transfer Assays

2003 ◽  
Vol 773 ◽  
Author(s):  
Aaron R. Clapp ◽  
Igor L. Medintz ◽  
J. Matthew Mauro ◽  
Hedi Mattoussi
Keyword(s):  
Energy Transfer ◽  
Quantum Dot ◽  
Organic Dyes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Genetically Engineered ◽  
Molar Ratio ◽  
Binding Assays ◽  
Specific Sequence ◽  
Donor Acceptor

AbstractLuminescent CdSe-ZnS core-shell quantum dot (QD) bioconjugates were used as energy donors in fluorescent resonance energy transfer (FRET) binding assays. The QDs were coated with saturating amounts of genetically engineered maltose binding protein (MBP) using a noncovalent immobilization process, and Cy3 organic dyes covalently attached at a specific sequence to MBP were used as energy acceptor molecules. Energy transfer efficiency was measured as a function of the MBP-Cy3/QD molar ratio for two different donor fluorescence emissions (different QD core sizes). Apparent donor-acceptor distances were determined from these FRET studies, and the measured distances are consistent with QD-protein conjugate dimensions previously determined from structural studies.

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