A biomimetic ZIF nanoagent for synergistic regulation of glutamine metabolism and intracellular acidosis of cancer

2022 ◽  
Author(s):  
Mengzhen Wang ◽  
Fei Lu ◽  
Na Li ◽  
Wei Pan ◽  
Bo Tang
Keyword(s):  
Cell Membrane ◽  
Cancer Cell ◽  
Glutamine Metabolism ◽  
Intracellular Acidosis ◽  
Zeolitic Imidazolate Framework ◽  
Synergistic Regulation

A homotypic cancer cell membrane camouflaged zeolitic imidazolate framework (ZIF)-based nanoagent with co-loading of two inhibitors has been developed, which could suppress the efflux of protons to induce intracellular acidic...

Theoretical Study of the Process of Passage of Glycoside Amides through the Cell Membrane of Cancer Cell

2020 ◽  
Vol 20 ◽  
Author(s):  
Vasil Tsanov ◽  
Hristo Tsanov
Keyword(s):  
Cell Membrane ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Quantum Chemical ◽  
Density Functional ◽  
Enzyme Regulation ◽  
Amide Derivatives ◽  
Pass Through ◽  
Hydrolysis Of ◽  
Derivatives Of

Background:: This article concentrates on the processes occurring in the medium around the cancer cell and the transfer of glycoside amides through their cell membrane. They are obtained by modification of natural glycoside-nitriles (cyano-glycosides). Hydrolysis of starting materials in the blood medium and associated volume around physiologically active healthy and cancer cells, based on quantum-chemical semi-empirical methods, is considered. Objective:: Based on the fact that the cancer cell feeds primarily on carbohydrates, it is likely that organisms have adapted to take food containing nitrile glycosides and / or modified forms to counteract "external" bioactive activity. Cancers, for their part, have evolved to create conditions around their cells that eliminate their active apoptotic forms. This is far more appropriate for them than changing their entire enzyme regulation to counteract it. In this way, it protects itself and the gene sets and develops according to its instructions. Methods:: Derived pedestal that closely defines the processes of hydrolysis in the blood, the transfer of a specific molecular hydrolytic form to the cancer cell membrane and with the help of time-dependent density-functional quantum- chemical methods, its passage and the processes of re-hydrolysis within the cell itself, to forms causing chemical apoptosis of the cell - independent of its non-genetic set, which seeks to counteract the process. Results:: Used in oncology it could turn a cancer from a lethal to a chronic disease (such as diabetes). The causative agent and conditions for the development of the disease are not eliminated, but the amount of cancer cells could be kept low for a long time (even a lifetime). Conclusion:: The amide derivatives of nitrile glycosides exhibit anti-cancer activity, the cancer cell probably seeks to displace hydrolysis of these derivatives in a direction that would not pass through its cell membrane and the amide- carboxyl derivatives of nitrile glycosides could deliver extremely toxic compounds within the cancer cell itself and thus block and / or permanently damage its normal physiology.

scholarly journals Carrier-free highly drug-loaded biomimetic nanosuspensions encapsulated by cancer cell membrane based on homology and active targeting for the treatment of glioma

2021 ◽  
Vol 6 (12) ◽  
pp. 4402-4414
Author(s):  
Yueyue Fan ◽  
Yuexin Cui ◽  
Wenyan Hao ◽  
Mengyu Chen ◽  
Qianqian Liu ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Cancer Cell ◽  
Active Targeting ◽  
Carrier Free

scholarly journals Investigation of an Optical Imaging Platform Integrated with an Ultrasound Application System for In Vitro Verification of Ultrasound-Mediated Drug Delivery

2021 ◽  
Vol 11 (6) ◽  
pp. 2846
Author(s):  
Jong-ryul Choi ◽  
Juyoung Park
Keyword(s):  
Drug Delivery ◽  
Cervical Cancer ◽  
Cell Membrane ◽  
Optical Imaging ◽  
Cancer Cell ◽  
Fluorescent Imaging ◽  
Calcium Flux ◽  
Cervical Cancer Cell ◽  
Application System ◽  
Ultrasound Application

Techniques that increase the permeability of the cell membrane and transfer drugs or genes to cells have been actively developed as effective therapeutic modalities. Also, in line with the development of these drug delivery techniques, the establishment of tools to verify the techniques at the cellular level is strongly required. In this study, we demonstrated an optical imaging platform integrated with an ultrasound application system to verify the feasibility of safe and efficient drug delivery through the cell membrane using ultrasound-microbubble cavitation. To examine the potential of the platform, fluorescence images of both Fura-2 AM and propidium iodide (PI) to measure calcium flux changes and intracellular PI delivery, respectively, during and after the ultrasound-microbubble cavitation in the cervical cancer cell were acquired. Using the optical imaging platform, we determined that calcium flux increased immediately after the ultrasound-microbubble cavitation and were restored to normal levels, and fluorescence signals from intracellular PI increased gradually after the cavitation. The results acquired by the platform indicated that ultrasound-microbubble cavitation can deliver PI into the cervical cancer cell without irreversible damage of the cell membrane. The application of an additional fluorescent imaging module and high-speed imaging modalities can provide further improvement of the performance of this platform. Also, as additional studies in ultrasound instrumentations to measure real-time cavitation signals progress, we believe that the ultrasound-microbubble cavitation-based sonoporation can be employed for safe and efficient drug and gene delivery to various cancer cells.

scholarly journals Aberrant Expression of Novel and Previously Described Cell Membrane Markers in Human Breast Cancer Cell Lines and Tumors

2005 ◽  
Vol 11 (12) ◽  
pp. 4357-4364 ◽  
Author(s):  
Huayi Huang ◽  
Jeff Groth ◽  
Khalid Sossey-Alaoui ◽  
Lesleyann Hawthorn ◽  
Stephanie Beall ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Membrane ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Human Breast Cancer ◽  
Human Breast Cancer Cell ◽  
Breast Cancer Cell Lines ◽  
Human Breast ◽  
Aberrant Expression

Irradiation conditioning of adjuvanted, autologous cancer cell membrane nanoparticle vaccines

2021 ◽  
pp. 134437
Author(s):  
Zhan Tuo ◽  
Qianyuan He ◽  
Zhanjie Zhang ◽  
Yiyu Wang ◽  
Jinfeng Sun ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Cancer Cell

Personalized Nanovaccine Coated with Calcinetin-Expressed Cancer Cell Membrane Antigen for Cancer Immunotherapy

Nano Letters ◽  
2021 ◽  
Author(s):  
Xiang Xiong ◽  
Jingya Zhao ◽  
Jingmei Pan ◽  
Chunping Liu ◽  
Xing Guo ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Cancer Immunotherapy ◽  
Cancer Cell ◽  
Cell Membrane Antigen

Cell membrane based biomimetic nanocomposites for targeted therapy of drug resistant EGFR-mutated lung cancer

Nanoscale ◽  
2019 ◽  
Vol 11 (41) ◽  
pp. 19520-19528 ◽  
Author(s):  
Pengying Wu ◽  
Dongtao Yin ◽  
Jiaming Liu ◽  
Huige Zhou ◽  
Mengyu Guo ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Drug Resistance ◽  
Targeted Therapy ◽  
Cell Membrane ◽  
Cancer Cell ◽  
Egfr Mutation ◽  
Drug Resistant ◽  
Egfr Mutated

A cancer cell membrane-based biomimetic strategy was developed by loading doxorubicin and icotinib to overcome drug-resistance of EGFR-mutation lung cancer.

Cancer cell membrane-cloaked mesoporous silica nanoparticles with a pH-sensitive gatekeeper for cancer treatment

2019 ◽  
Vol 175 ◽  
pp. 477-486 ◽  
Author(s):  
Chang-Ming Liu ◽  
Guang-Bing Chen ◽  
Hui-Hong Chen ◽  
Jia-Bin Zhang ◽  
Hui-Zhang Li ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Mesoporous Silica ◽  
Cancer Treatment ◽  
Silica Nanoparticles ◽  
Cancer Cell ◽  
Mesoporous Silica Nanoparticles ◽  
Ph Sensitive

scholarly journals Causes and Consequences of A Glutamine Induced Normoxic HIF1 Activity for the Tumor Metabolism

2019 ◽  
Vol 20 (19) ◽  
pp. 4742 ◽  
Author(s):  
Matthias Kappler ◽  
Ulrike Pabst ◽  
Claus Weinholdt ◽  
Helge Taubert ◽  
Swetlana Rot ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Ascorbic Acid ◽  
Amino Acid ◽  
Tumor Cells ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Glutamine Metabolism ◽  
Dependent Manner ◽  
Acetyl Coa ◽  
Hypoxic Conditions

The transcription factor hypoxia-inducible factor 1 (HIF1) is the crucial regulator of genes that are involved in metabolism under hypoxic conditions, but information regarding the transcriptional activity of HIF1 in normoxic metabolism is limited. Different tumor cells were treated under normoxic and hypoxic conditions with various drugs that affect cellular metabolism. HIF1α was silenced by siRNA in normoxic/hypoxic tumor cells, before RNA sequencing and bioinformatics analyses were performed while using the breast cancer cell line MDA-MB-231 as a model. Differentially expressed genes were further analyzed and validated by qPCR, while the activity of the metabolites was determined by enzyme assays. Under normoxic conditions, HIF1 activity was significantly increased by (i) glutamine metabolism, which was associated with the release of ammonium, and it was decreased by (ii) acetylation via acetyl CoA synthetase (ACSS2) or ATP citrate lyase (ACLY), respectively, and (iii) the presence of L-ascorbic acid, citrate, or acetyl-CoA. Interestingly, acetylsalicylic acid, ibuprofen, L-ascorbic acid, and citrate each significantly destabilized HIF1α only under normoxia. The results from the deep sequence analyses indicated that, in HIF1-siRNA silenced MDA-MB-231 cells, 231 genes under normoxia and 1384 genes under hypoxia were transcriptionally significant deregulated in a HIF1-dependent manner. Focusing on glycolysis genes, it was confirmed that HIF1 significantly regulated six normoxic and 16 hypoxic glycolysis-associated gene transcripts. However, the results from the targeted metabolome analyses revealed that HIF1 activity affected neither the consumption of glucose nor the release of ammonium or lactate; however, it significantly inhibited the release of the amino acid alanine. This study comprehensively investigated, for the first time, how normoxic HIF1 is stabilized, and it analyzed the possible function of normoxic HIF1 in the transcriptome and metabolic processes of tumor cells in a breast cancer cell model. Furthermore, these data imply that HIF1 compensates for the metabolic outcomes of glutaminolysis and, subsequently, the Warburg effect might be a direct consequence of the altered amino acid metabolism in tumor cells.

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